Cell printing is an emerging technology for engineering complex 3D tissue/organ structures. In th... more Cell printing is an emerging technology for engineering complex 3D tissue/organ structures. In this study, the proliferation of primary rat bladder smooth muscle cells (SMCs) in printed cell-laden collagen droplets was quantified. The results showed that the bioprinting system is capable of printing viable cells, while keeping their proliferation ability in a 3D collagen matrix.
Tissue engineering based on building blocks is an emerging method to fabricate 3D tissue construc... more Tissue engineering based on building blocks is an emerging method to fabricate 3D tissue constructs. This method requires depositing and assembling building blocks (cell-laden microgels) at high throughput. The current technologies (e.g., molding and photolithography) to fabricate microgels have throughput challenges and provide limited control over building block properties (e.g., cell density). The cell-encapsulating droplet generation technique has potential to address these challenges. In this study, we monitored individual building blocks for viability, proliferation and cell density. The results showed that (i) SMCs can be encapsulated in collagen droplets with high viability (>94.2 +/- 3.2%) for four cases of initial number of cells per building block (i.e. 7 +/- 2, 16 +/- 2, 26 +/- 3 and 37 +/- 3 cells/building block). (ii) Encapsulated SMCs can proliferate in building blocks at rates that are consistent (1.49 +/- 0.29) across all four cases, compared to that of the contr...
Cell printing is an emerging technology for engineering complex 3D tissue/organ structures. In th... more Cell printing is an emerging technology for engineering complex 3D tissue/organ structures. In this study, the proliferation of primary rat bladder smooth muscle cells (SMCs) in printed cell-laden collagen droplets was quantified. The results showed that the bioprinting system is capable of printing viable cells, while keeping their proliferation ability in a 3D collagen matrix.
Tissue engineering based on building blocks is an emerging method to fabricate 3D tissue construc... more Tissue engineering based on building blocks is an emerging method to fabricate 3D tissue constructs. This method requires depositing and assembling building blocks (cell-laden microgels) at high throughput. The current technologies (e.g., molding and photolithography) to fabricate microgels have throughput challenges and provide limited control over building block properties (e.g., cell density). The cell-encapsulating droplet generation technique has potential to address these challenges. In this study, we monitored individual building blocks for viability, proliferation and cell density. The results showed that (i) SMCs can be encapsulated in collagen droplets with high viability (>94.2 +/- 3.2%) for four cases of initial number of cells per building block (i.e. 7 +/- 2, 16 +/- 2, 26 +/- 3 and 37 +/- 3 cells/building block). (ii) Encapsulated SMCs can proliferate in building blocks at rates that are consistent (1.49 +/- 0.29) across all four cases, compared to that of the contr...
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Papers by E. Turali