Uniparental silencing of 35S rRNA genes (rDNA), known as nucleolar dominance (ND), is common in i... more Uniparental silencing of 35S rRNA genes (rDNA), known as nucleolar dominance (ND), is common in interspecific hybrids. Tragopogon mirus allotetraploid composed of T. dubius (d) and T. porrifolius (p) genomes show highly variable ND. To examine the molecular basis of such variation, we studied the genetic and epigenetic features of rDNA homeologs in several lines derived from recently and independently formed natural populations. Inbred lines derived from the T. mirus with dominant d-rDNA homeolog transmitted this expression pattern over generations, which may explain why it is prevalent among natural populations. In contrast, lines derived from the p-rDNA dominant progenitor were meiotically unstable, frequently switching to a codominance. Interpopulation crosses between progenitors displaying reciprocal ND resulted in d-rDNA dominance, indicating immediate suppression of p-homeologs in F1 hybrids. Original p-rDNA dominance was not restored in later generations, even in those segregants that inherited the corresponding parental rDNA genotype, thus indicating the generation of additional p-rDNA and d-rDNA epigenetic variants. Despite preserved intergenic spacer (IGS) structure, they showed altered cytosine methylation and chromatin condensation patterns, and a correlation between expression, hypomethylation of RNA Pol I promoters and chromatin decondensation was apparent. Reversion of such epigenetic variants occurred rarely, resulting in codominance maintained in individuals with distinct genotypes. Generally, interpopulation crosses may generate epialleles not present in natural populations, underlying epigenetic dynamics in young allopolyploids. We hypothesize that highly expressed variants with distinct IGS features may induce heritable epigenetic reprogramming of the partner rDNA arrays, harmonizing the expression of thousands of genes in allopolyploids. This article is protected by copyright. All rights reserved.
Pharmacological Research the Official Journal of the Italian Pharmacological Society, Feb 1, 2010
Magnetic nanoparticles (MNPs) are being of great interest due to their unique purposes. Especiall... more Magnetic nanoparticles (MNPs) are being of great interest due to their unique purposes. Especially in medicine, application of MNPs is much promising. MNPs have been actively investigated as the next generation of targeted drug delivery for more than thirty years. The importance of targeted drug delivery and targeted drug therapy is to transport a drug directly to the centre of the disease under various conditions and thereby treat it deliberately, with no effects on the body. Usage of MNPs depends largely on the preparation processes to select optimal conditions and election agents to modify their surface. This review summarizes the most commonly used functionalization methods of the MNPs preparation methods and their use in targeted drug delivery and targeted therapy.
1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemed... more 1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic 2 Tomas Bata University, TG Masaryka 275, CZ-762 72 Zlin, Czech Republic 3 Department of Veterinary Ecology and ...
Egu General Assembly Conference Abstracts, Apr 1, 2009
Abstract In a number of published articles the toxic effect of silver (I) ions on aquatic organis... more Abstract In a number of published articles the toxic effect of silver (I) ions on aquatic organisms is described. Silver (I) ions in aquatic environment are stable in a wide range of pH. Under alkali pH AgOH and Ag (OH) 2-can be formed. However, in water environment ...
Résumé/Abstract Manual handling with DNA and drugs can be a source of errors, which can be elimin... more Résumé/Abstract Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interactian between DNA and ellipticine by using of off-line coupled ...
Title: Accumulation and hyperaccumulation of copper in plants. Authors: Adam, V.; Trnkova, L.; Hu... more Title: Accumulation and hyperaccumulation of copper in plants. Authors: Adam, V.; Trnkova, L.; Huska, D.; Babula, P.; Kizek, R. Affiliation: AA(Masaryk University, Department of Chemistry, Brno, Czech Republic (kizek@sci.muni ...
... Basic information. Original title: Relationship of metalloproteinases with metallothioneins. ... more ... Basic information. Original title: Relationship of metalloproteinases with metallothioneins. Authors: Michal Masařík, Ondřej Zítka, Dalibor Húska, Soňa Kříková, Vojtěch Adam, Jiří Kukačka, Richard Průa, Věra iková, René Kizek. Further information. ...
... Basic information. Original title: Square wave voltammetry as a tool for study of ellipticine... more ... Basic information. Original title: Square wave voltammetry as a tool for study of ellipticine -DNA. Authors: Michal Masařík, Dalibor Húska, Vojtěch Adam, Jaromír Hubálek, Ivo Provazník, Libue Trnková, Tomá Eckschlager, Marie Stiborová, René Kizek. Further information. ...
The aim of this work was to propose new technique for isolation and detection of total content of... more The aim of this work was to propose new technique for isolation and detection of total content of mRNA in human plasma. The total content of plasma mRNA varied from 0.1 to 1.2 ug/ml. We succeeded in isolating mRNA by using simple technique with MPs. Recovery of the isolation of mRNA (tested on polyA chains) was about 30 %. In comparison with common techniques used for isolation of mRNA (e.g. phenol-chloroform extraction) the recovery of the MPs approach is higher and, moreover, this technique is not so time consuming, because we were able to analyse twelve plasma samples within 120 minutes.
Uniparental silencing of 35S rRNA genes (rDNA), known as nucleolar dominance (ND), is common in i... more Uniparental silencing of 35S rRNA genes (rDNA), known as nucleolar dominance (ND), is common in interspecific hybrids. Tragopogon mirus allotetraploid composed of T. dubius (d) and T. porrifolius (p) genomes show highly variable ND. To examine the molecular basis of such variation, we studied the genetic and epigenetic features of rDNA homeologs in several lines derived from recently and independently formed natural populations. Inbred lines derived from the T. mirus with dominant d-rDNA homeolog transmitted this expression pattern over generations, which may explain why it is prevalent among natural populations. In contrast, lines derived from the p-rDNA dominant progenitor were meiotically unstable, frequently switching to a codominance. Interpopulation crosses between progenitors displaying reciprocal ND resulted in d-rDNA dominance, indicating immediate suppression of p-homeologs in F1 hybrids. Original p-rDNA dominance was not restored in later generations, even in those segregants that inherited the corresponding parental rDNA genotype, thus indicating the generation of additional p-rDNA and d-rDNA epigenetic variants. Despite preserved intergenic spacer (IGS) structure, they showed altered cytosine methylation and chromatin condensation patterns, and a correlation between expression, hypomethylation of RNA Pol I promoters and chromatin decondensation was apparent. Reversion of such epigenetic variants occurred rarely, resulting in codominance maintained in individuals with distinct genotypes. Generally, interpopulation crosses may generate epialleles not present in natural populations, underlying epigenetic dynamics in young allopolyploids. We hypothesize that highly expressed variants with distinct IGS features may induce heritable epigenetic reprogramming of the partner rDNA arrays, harmonizing the expression of thousands of genes in allopolyploids. This article is protected by copyright. All rights reserved.
Pharmacological Research the Official Journal of the Italian Pharmacological Society, Feb 1, 2010
Magnetic nanoparticles (MNPs) are being of great interest due to their unique purposes. Especiall... more Magnetic nanoparticles (MNPs) are being of great interest due to their unique purposes. Especially in medicine, application of MNPs is much promising. MNPs have been actively investigated as the next generation of targeted drug delivery for more than thirty years. The importance of targeted drug delivery and targeted drug therapy is to transport a drug directly to the centre of the disease under various conditions and thereby treat it deliberately, with no effects on the body. Usage of MNPs depends largely on the preparation processes to select optimal conditions and election agents to modify their surface. This review summarizes the most commonly used functionalization methods of the MNPs preparation methods and their use in targeted drug delivery and targeted therapy.
1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemed... more 1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic 2 Tomas Bata University, TG Masaryka 275, CZ-762 72 Zlin, Czech Republic 3 Department of Veterinary Ecology and ...
Egu General Assembly Conference Abstracts, Apr 1, 2009
Abstract In a number of published articles the toxic effect of silver (I) ions on aquatic organis... more Abstract In a number of published articles the toxic effect of silver (I) ions on aquatic organisms is described. Silver (I) ions in aquatic environment are stable in a wide range of pH. Under alkali pH AgOH and Ag (OH) 2-can be formed. However, in water environment ...
Résumé/Abstract Manual handling with DNA and drugs can be a source of errors, which can be elimin... more Résumé/Abstract Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interactian between DNA and ellipticine by using of off-line coupled ...
Title: Accumulation and hyperaccumulation of copper in plants. Authors: Adam, V.; Trnkova, L.; Hu... more Title: Accumulation and hyperaccumulation of copper in plants. Authors: Adam, V.; Trnkova, L.; Huska, D.; Babula, P.; Kizek, R. Affiliation: AA(Masaryk University, Department of Chemistry, Brno, Czech Republic (kizek@sci.muni ...
... Basic information. Original title: Relationship of metalloproteinases with metallothioneins. ... more ... Basic information. Original title: Relationship of metalloproteinases with metallothioneins. Authors: Michal Masařík, Ondřej Zítka, Dalibor Húska, Soňa Kříková, Vojtěch Adam, Jiří Kukačka, Richard Průa, Věra iková, René Kizek. Further information. ...
... Basic information. Original title: Square wave voltammetry as a tool for study of ellipticine... more ... Basic information. Original title: Square wave voltammetry as a tool for study of ellipticine -DNA. Authors: Michal Masařík, Dalibor Húska, Vojtěch Adam, Jaromír Hubálek, Ivo Provazník, Libue Trnková, Tomá Eckschlager, Marie Stiborová, René Kizek. Further information. ...
The aim of this work was to propose new technique for isolation and detection of total content of... more The aim of this work was to propose new technique for isolation and detection of total content of mRNA in human plasma. The total content of plasma mRNA varied from 0.1 to 1.2 ug/ml. We succeeded in isolating mRNA by using simple technique with MPs. Recovery of the isolation of mRNA (tested on polyA chains) was about 30 %. In comparison with common techniques used for isolation of mRNA (e.g. phenol-chloroform extraction) the recovery of the MPs approach is higher and, moreover, this technique is not so time consuming, because we were able to analyse twelve plasma samples within 120 minutes.
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