Simple Summary Chemoresistance of bladder cancer has become a major obstacle to clinical treatmen... more Simple Summary Chemoresistance of bladder cancer has become a major obstacle to clinical treatment, especially in first-line treatments involving gemcitabine (GCB). Epithelial-mesenchymal transition (EMT) is highly correlated with GCB resistance but less correlated with GCB metabolism and less reported as a novel therapeutic strategy. Our findings indicated that EMT-related GCB resistance occurs through the TGF-β/Smad signaling pathways and involves repressed expression of the GCB transporters hCNT1 and hENT1. Ellagic acid (EA) combined with GCB intensified the chemosensitivity of GCB in resistant cells by repressing Smad2, Smad3, and Smad4 expression and rescuing hCNT1 and hENT transcription. These data suggest that EA is a good adjuvant agent for blocking TGF-β/Smad signaling-related GCB resistance in bladder cancer. Abstract Gemcitabine (GCB) resistance is a major issue in bladder cancer chemoresistance, but its underlying mechanism has not been determined. Epithelial-mesenchymal...
... Ta iwan U rological Association 1978 Page 2. Robotic Surgery in Taiwan JTUA 2007 18 No. 4 180... more ... Ta iwan U rological Association 1978 Page 2. Robotic Surgery in Taiwan JTUA 2007 18 No. 4 180 for hip prostheses. The first commercial robotic system used specifically for laparoscopic applications was the Automated Endoscopic System for Optimal Position-ing (AESOP). ...
Patients with multiple endocrine neoplasia (MEN) type II disease are predisposed to develop bilat... more Patients with multiple endocrine neoplasia (MEN) type II disease are predisposed to develop bilateral adrenal pheochromocytomas. The standard treatment is a bilateral adrenalectomy in these patients. However, an adrenal-sparing operation preserves adrenocortical function and avoids the morbidity associated with cortisol replacement therapy. The issue of partial adrenal resection in these patients needs further evaluation. Since the first report on laparoscopic adrenalectomy, laparoscopy has become the gold standard technique for surgical treatment of benign adrenal tumors. Laparoscopic resection of pheochromocytomas is feasible but potentially more challenging because of the risks for intraoperative hemodynamic instability and the usually large size of these tumors. Only a few reports have shown that a laparoscopic partial adrenalectomy is feasible and has few perioperative complications. We report our method and how we managed the bilateral pheochromocytomas in a 39-year-old women ...
Journal of the Formosan Medical Association = Taiwan yi zhi, 2003
The expression of specific CD44 isoforms is associated with metastasis and poor prognosis in huma... more The expression of specific CD44 isoforms is associated with metastasis and poor prognosis in human malignant tumors. We retrospectively investigated the expression of the CD44 variant isoform v5 (CD44v5) in human renal cell carcinoma (RCC) specimens by immunohistochemistry. We studied the expression of CD44v5 immunohistochemistry in archival tissue specimens from 72 RCC patients (47 men and 25 women) with a mean age of 60.1 years (range, 30 to 83 years) who underwent resection in our hospital from 1986 to 1996. The patients were divided into non-invasive (pT1, pT2, and pT3a; n = 49) and invasive groups (pT3b, pT3c, and any T with N+; n = 23). Among the 72 patients, 67 were followed for at least 60 months (up to 190 months) after radical nephrectomy. Twenty nine renal specimens, including 15 of normal renal parenchyma and 14 of inflammatory or immune renal diseases, were used as controls. All control group tissues expressed CD44v5. CD44v5 expression was detected in 66 of 72 RCC speci...
Although hemangiomas are the most common benign tumors in infancy, scrotal hemangiomas are extrem... more Although hemangiomas are the most common benign tumors in infancy, scrotal hemangiomas are extremely rare and comprise less than 1% of all hemangiomas. Scrotal hemangiomas that extend into adjacent areas of the perineum, thigh, or anterior abdominal wall may occasionally be seen. Ultrasound is recommended as part of the preoperative assessment delineating the extent of a scrotal hemangioma. Since an absence of flow on Doppler studies does not exclude the diagnosis of hemangioma, MRI (magnetic resonance imaging) may provide more useful information for differentiation. In cases of cutaneous scrotal hemangiomas, conservative treatment that waits for involution is widely accepted. In patients with scrotal masses, exploration with excision is the treatment of choice even if a hemangioma is likely. The authors report a case of an intrascrotal tumor diagnosed preoperatively by color duplex ultrasonography and MRI in a 19-year-old male who subsequently underwent en bloc excision. Pathological examination identified a cavernous hemangioma.
Cell-mediated cytotoxicity in patients with urologic cancer was studied using the K562 cell line ... more Cell-mediated cytotoxicity in patients with urologic cancer was studied using the K562 cell line as target cell by a 4-hour chromium-51 release assay. Lysis of target cells by mononuclear cells of a healthy subject, over an 8-hour incubation period, demonstrated a linear function of incubation time and effector:target ratio. Natural killer (NK) cell activity was found decreased (mean 30.6%) in peripheral blood lymphocytes from 42 untreated patients with urologic cancer when compared to 20 healthy subjects (63.6%) and to 10 patients with varicocele, stone disease and benign prostatic hypertrophy (58.1%; p less than 0.05). There was no correlation between NK cell activity and the grade or stage status in bladder cancer patients. No age-dependent changes in NK cell activity could be found between young and aged groups of healthy subjects. Healthy male subjects have higher levels of NK cell activity (77.7%) than healthy female subjects (49.5%). Postoperative NK activity rose in 5 out of 6 cancer patients. It indicates that tumors may have an inhibitory effect on the surveillance activity of NK cells.
An IgG3 murine monoclonal antibody 1G3.10 was generated against a human urinary bladder carcinoma... more An IgG3 murine monoclonal antibody 1G3.10 was generated against a human urinary bladder carcinoma cell line TSGH-8301. The tumor-associated antigen recognized by the monoclonal antibody is a blood group A-related substance. From fluorescence-activated cell sorter analysis, a direct cytolytic effect of tumor cells by the antibody at concentrations of more than 10 micrograms/ml was seen. The antibody could target iodine-131 to established subcutaneous human bladder carcinoma xenografts in BALB/c nude mice. Monoclonal antibody alone also showed some inhibitory effect on xenografted tumor growth. In this study, radiolabeled monoclonal antibody 1G3.10 presented more effective cytotoxicity than antibody alone from in vitro and in vivo experiments.
European journal of medicinal chemistry, Jan 18, 2015
A series of sulfur-substituted anthra[1,2-c][1,2,5]thiadiazole-6,11-diones were synthesized and e... more A series of sulfur-substituted anthra[1,2-c][1,2,5]thiadiazole-6,11-diones were synthesized and evaluated using the cell proliferations, apoptosis and NCI-60 cell panel assays. Also, the signaling pathways that account for their activities were investigated. Compounds 2, 3, 4a, 4d, 4f, 4i, 4k, 5b, 5c, 5d, 5f, 5g, 6b, 6c, 6d, 6e, 6g, 7a and 7g were selected by NCI. Among the tested compounds, 6g appeared to be the most active compound of this series that not only induced apoptosis in DU-145 cancer cells but also attenuated the ERK1/2 and p38 signaling pathways. All test compounds exhibited diverse cytostatic and cytotoxic activities that warrant further development as potential anticancer agents.
Fluorescence in situ hybridization (FISH) was used to study numerical aberrations of chromosomes ... more Fluorescence in situ hybridization (FISH) was used to study numerical aberrations of chromosomes 1, 3, 7, 9, 11, 15, 17, X and Y in interphase nuclei of 16 transitional cell carcinoma (TCC) cell lines of the urinary tract. The number of chromosomal signal copies was compared with the DNA ploidy and correlated with the cellular grading and original tumor staging. The single-target FISH with the repetitive DNA probes for chromosomes 1, 3, 5, 7, 9, 11, 15, 17, X and Y were performed in 16 human TCC cell lines. For each test specimen, a minimum of 200 nuclei were analyzed. The number of fluorescent signals per nucleus was recorded. Tumor ploidy was analyzed using the DNA propidium iodide flow cytometric method. These results were correlated with tumor grade and stage. In two diploid TCC cell lines, on average 67% of detected chromosomes were di-(i)somic. In 14 aneuploid TCC cell lines, on average only 10% (0-44%) of detected chromosomes were di-(i)somic. Chromosome X was completely chan...
European journal of medicinal chemistry, Jan 16, 2014
A series of tetracyclic heterocyclic azathioxanthones were synthesized and evaluated for cell pro... more A series of tetracyclic heterocyclic azathioxanthones were synthesized and evaluated for cell proliferations, topoisomerase inhibitions, and NCI-60 cell panel assay, respectively. Compounds 5, 7, 8, 16, and 19 were selected for topoisomerase assay after MTT assay. 7 not only showed cytotoxic effect (IC50 = 2.84 ± 0.64 μM) in PC-3 cells but also revealed topoisomerases inhibition with IC50 (10-25 μM) and increased apoptotic cleavage of PARP and caspase 3 activity. The overall of novel azathioxanthones with different cytostatic and cytotoxic activities should be further developed as new potential candidates for anticancer drugs.
Proceedings of the National Science Council, Republic of China. Part B, Life sciences, 1988
A mouse monoclonal antibody, designated 1G3.10, directed against a human urinary bladder cancer c... more A mouse monoclonal antibody, designated 1G3.10, directed against a human urinary bladder cancer cell line TSGH-8301 was generated using the conventional hybridoma technique. It was of the IgG3 subclass with a restricted specificity to bladder cancer and several epithelial cancers, as detected on a panel of various cell lines and tissues by radioimmunoassay and indirect immunofluorescence methods. It also exhibited a strong binding activity to human group A red blood cells, as demonstrated in hemagglutination and absorption tests. Radioimmunoprecipitation and thin-layer chromatography revealed that the antigens recognized by the antibody are a mixture of glycopeptides (M.W. 30, 54, and 108 KD) and glycolipid (retardation factor 0.67-0.83). From a fluorescence activated cell sorter (FACS) study, direct killing of tumor cells by the antibody at a concentration of more than 10 micrograms/ml was observed. This antibody could also mediate both antibody-dependent cellular cytotoxicity (ADC...
Simple Summary Chemoresistance of bladder cancer has become a major obstacle to clinical treatmen... more Simple Summary Chemoresistance of bladder cancer has become a major obstacle to clinical treatment, especially in first-line treatments involving gemcitabine (GCB). Epithelial-mesenchymal transition (EMT) is highly correlated with GCB resistance but less correlated with GCB metabolism and less reported as a novel therapeutic strategy. Our findings indicated that EMT-related GCB resistance occurs through the TGF-β/Smad signaling pathways and involves repressed expression of the GCB transporters hCNT1 and hENT1. Ellagic acid (EA) combined with GCB intensified the chemosensitivity of GCB in resistant cells by repressing Smad2, Smad3, and Smad4 expression and rescuing hCNT1 and hENT transcription. These data suggest that EA is a good adjuvant agent for blocking TGF-β/Smad signaling-related GCB resistance in bladder cancer. Abstract Gemcitabine (GCB) resistance is a major issue in bladder cancer chemoresistance, but its underlying mechanism has not been determined. Epithelial-mesenchymal...
... Ta iwan U rological Association 1978 Page 2. Robotic Surgery in Taiwan JTUA 2007 18 No. 4 180... more ... Ta iwan U rological Association 1978 Page 2. Robotic Surgery in Taiwan JTUA 2007 18 No. 4 180 for hip prostheses. The first commercial robotic system used specifically for laparoscopic applications was the Automated Endoscopic System for Optimal Position-ing (AESOP). ...
Patients with multiple endocrine neoplasia (MEN) type II disease are predisposed to develop bilat... more Patients with multiple endocrine neoplasia (MEN) type II disease are predisposed to develop bilateral adrenal pheochromocytomas. The standard treatment is a bilateral adrenalectomy in these patients. However, an adrenal-sparing operation preserves adrenocortical function and avoids the morbidity associated with cortisol replacement therapy. The issue of partial adrenal resection in these patients needs further evaluation. Since the first report on laparoscopic adrenalectomy, laparoscopy has become the gold standard technique for surgical treatment of benign adrenal tumors. Laparoscopic resection of pheochromocytomas is feasible but potentially more challenging because of the risks for intraoperative hemodynamic instability and the usually large size of these tumors. Only a few reports have shown that a laparoscopic partial adrenalectomy is feasible and has few perioperative complications. We report our method and how we managed the bilateral pheochromocytomas in a 39-year-old women ...
Journal of the Formosan Medical Association = Taiwan yi zhi, 2003
The expression of specific CD44 isoforms is associated with metastasis and poor prognosis in huma... more The expression of specific CD44 isoforms is associated with metastasis and poor prognosis in human malignant tumors. We retrospectively investigated the expression of the CD44 variant isoform v5 (CD44v5) in human renal cell carcinoma (RCC) specimens by immunohistochemistry. We studied the expression of CD44v5 immunohistochemistry in archival tissue specimens from 72 RCC patients (47 men and 25 women) with a mean age of 60.1 years (range, 30 to 83 years) who underwent resection in our hospital from 1986 to 1996. The patients were divided into non-invasive (pT1, pT2, and pT3a; n = 49) and invasive groups (pT3b, pT3c, and any T with N+; n = 23). Among the 72 patients, 67 were followed for at least 60 months (up to 190 months) after radical nephrectomy. Twenty nine renal specimens, including 15 of normal renal parenchyma and 14 of inflammatory or immune renal diseases, were used as controls. All control group tissues expressed CD44v5. CD44v5 expression was detected in 66 of 72 RCC speci...
Although hemangiomas are the most common benign tumors in infancy, scrotal hemangiomas are extrem... more Although hemangiomas are the most common benign tumors in infancy, scrotal hemangiomas are extremely rare and comprise less than 1% of all hemangiomas. Scrotal hemangiomas that extend into adjacent areas of the perineum, thigh, or anterior abdominal wall may occasionally be seen. Ultrasound is recommended as part of the preoperative assessment delineating the extent of a scrotal hemangioma. Since an absence of flow on Doppler studies does not exclude the diagnosis of hemangioma, MRI (magnetic resonance imaging) may provide more useful information for differentiation. In cases of cutaneous scrotal hemangiomas, conservative treatment that waits for involution is widely accepted. In patients with scrotal masses, exploration with excision is the treatment of choice even if a hemangioma is likely. The authors report a case of an intrascrotal tumor diagnosed preoperatively by color duplex ultrasonography and MRI in a 19-year-old male who subsequently underwent en bloc excision. Pathological examination identified a cavernous hemangioma.
Cell-mediated cytotoxicity in patients with urologic cancer was studied using the K562 cell line ... more Cell-mediated cytotoxicity in patients with urologic cancer was studied using the K562 cell line as target cell by a 4-hour chromium-51 release assay. Lysis of target cells by mononuclear cells of a healthy subject, over an 8-hour incubation period, demonstrated a linear function of incubation time and effector:target ratio. Natural killer (NK) cell activity was found decreased (mean 30.6%) in peripheral blood lymphocytes from 42 untreated patients with urologic cancer when compared to 20 healthy subjects (63.6%) and to 10 patients with varicocele, stone disease and benign prostatic hypertrophy (58.1%; p less than 0.05). There was no correlation between NK cell activity and the grade or stage status in bladder cancer patients. No age-dependent changes in NK cell activity could be found between young and aged groups of healthy subjects. Healthy male subjects have higher levels of NK cell activity (77.7%) than healthy female subjects (49.5%). Postoperative NK activity rose in 5 out of 6 cancer patients. It indicates that tumors may have an inhibitory effect on the surveillance activity of NK cells.
An IgG3 murine monoclonal antibody 1G3.10 was generated against a human urinary bladder carcinoma... more An IgG3 murine monoclonal antibody 1G3.10 was generated against a human urinary bladder carcinoma cell line TSGH-8301. The tumor-associated antigen recognized by the monoclonal antibody is a blood group A-related substance. From fluorescence-activated cell sorter analysis, a direct cytolytic effect of tumor cells by the antibody at concentrations of more than 10 micrograms/ml was seen. The antibody could target iodine-131 to established subcutaneous human bladder carcinoma xenografts in BALB/c nude mice. Monoclonal antibody alone also showed some inhibitory effect on xenografted tumor growth. In this study, radiolabeled monoclonal antibody 1G3.10 presented more effective cytotoxicity than antibody alone from in vitro and in vivo experiments.
European journal of medicinal chemistry, Jan 18, 2015
A series of sulfur-substituted anthra[1,2-c][1,2,5]thiadiazole-6,11-diones were synthesized and e... more A series of sulfur-substituted anthra[1,2-c][1,2,5]thiadiazole-6,11-diones were synthesized and evaluated using the cell proliferations, apoptosis and NCI-60 cell panel assays. Also, the signaling pathways that account for their activities were investigated. Compounds 2, 3, 4a, 4d, 4f, 4i, 4k, 5b, 5c, 5d, 5f, 5g, 6b, 6c, 6d, 6e, 6g, 7a and 7g were selected by NCI. Among the tested compounds, 6g appeared to be the most active compound of this series that not only induced apoptosis in DU-145 cancer cells but also attenuated the ERK1/2 and p38 signaling pathways. All test compounds exhibited diverse cytostatic and cytotoxic activities that warrant further development as potential anticancer agents.
Fluorescence in situ hybridization (FISH) was used to study numerical aberrations of chromosomes ... more Fluorescence in situ hybridization (FISH) was used to study numerical aberrations of chromosomes 1, 3, 7, 9, 11, 15, 17, X and Y in interphase nuclei of 16 transitional cell carcinoma (TCC) cell lines of the urinary tract. The number of chromosomal signal copies was compared with the DNA ploidy and correlated with the cellular grading and original tumor staging. The single-target FISH with the repetitive DNA probes for chromosomes 1, 3, 5, 7, 9, 11, 15, 17, X and Y were performed in 16 human TCC cell lines. For each test specimen, a minimum of 200 nuclei were analyzed. The number of fluorescent signals per nucleus was recorded. Tumor ploidy was analyzed using the DNA propidium iodide flow cytometric method. These results were correlated with tumor grade and stage. In two diploid TCC cell lines, on average 67% of detected chromosomes were di-(i)somic. In 14 aneuploid TCC cell lines, on average only 10% (0-44%) of detected chromosomes were di-(i)somic. Chromosome X was completely chan...
European journal of medicinal chemistry, Jan 16, 2014
A series of tetracyclic heterocyclic azathioxanthones were synthesized and evaluated for cell pro... more A series of tetracyclic heterocyclic azathioxanthones were synthesized and evaluated for cell proliferations, topoisomerase inhibitions, and NCI-60 cell panel assay, respectively. Compounds 5, 7, 8, 16, and 19 were selected for topoisomerase assay after MTT assay. 7 not only showed cytotoxic effect (IC50 = 2.84 ± 0.64 μM) in PC-3 cells but also revealed topoisomerases inhibition with IC50 (10-25 μM) and increased apoptotic cleavage of PARP and caspase 3 activity. The overall of novel azathioxanthones with different cytostatic and cytotoxic activities should be further developed as new potential candidates for anticancer drugs.
Proceedings of the National Science Council, Republic of China. Part B, Life sciences, 1988
A mouse monoclonal antibody, designated 1G3.10, directed against a human urinary bladder cancer c... more A mouse monoclonal antibody, designated 1G3.10, directed against a human urinary bladder cancer cell line TSGH-8301 was generated using the conventional hybridoma technique. It was of the IgG3 subclass with a restricted specificity to bladder cancer and several epithelial cancers, as detected on a panel of various cell lines and tissues by radioimmunoassay and indirect immunofluorescence methods. It also exhibited a strong binding activity to human group A red blood cells, as demonstrated in hemagglutination and absorption tests. Radioimmunoprecipitation and thin-layer chromatography revealed that the antigens recognized by the antibody are a mixture of glycopeptides (M.W. 30, 54, and 108 KD) and glycolipid (retardation factor 0.67-0.83). From a fluorescence activated cell sorter (FACS) study, direct killing of tumor cells by the antibody at a concentration of more than 10 micrograms/ml was observed. This antibody could also mediate both antibody-dependent cellular cytotoxicity (ADC...
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