Papers by Concepcion Capetillo
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Tree fodders, polyphenolic compounds, tannins, biological activity, in vitro gas production, vari... more Tree fodders, polyphenolic compounds, tannins, biological activity, in vitro gas production, variability.
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Measurement of L-canavanine by reverse phase high performance liquid chromatography and by an spe... more Measurement of L-canavanine by reverse phase high performance liquid chromatography and by an spectrofotometric method.
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Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Apdo. 4-116, Itzim... more Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Apdo. 4-116, Itzimná, Mérida, Yucatán, México.Email: ccastro@uady.mx *Corresponding author ... The in vitro fermentation efficiency of Cynodon nlemfuensis forage (star grass) and ...
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Se determinó la digestibilidad ileal aparente de aminoácidos en maíz, sorgo, pasta de soya, glute... more Se determinó la digestibilidad ileal aparente de aminoácidos en maíz, sorgo, pasta de soya, gluten de maíz, harina de pescado, harina de carne y hueso, y salvado de trigo en pollos de engorda, utilizando dióxido de titanio y óxido de cromo como marcadores inertes de la dieta. Doce pollos fueron alimentados en dos ocasiones con 30g de cada ingrediente, con uno de los dos marcadores dietéticos, mediante la técnica de alimentación forzada. Se llevaron a cabo análisis de varianza con los resultados para determinar efectos de ingredientes y de marcadores. Se encontraron diferencias significativas en la digestibilidad de aminoácidos entre ingredientes, cuyo rango fue de 0,53 para cisteína en la harina de carne y hueso, hasta 0,97 para tirosina en pasta de soya. En todos los ingredientes los coeficientes de digestibilidad de aminoácidos calculados con base en titanio fueron mayores (P<0,05) que aquellos determinados con base en cromo. La excepción fue para salvado de trigo donde no se e...
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Thirty-six broiler chickens (COBB comercial line) four-week old were used to assess the apparent ... more Thirty-six broiler chickens (COBB comercial line) four-week old were used to assess the apparent ileal digestibility of protein and amino acids with the dietary inclusion of mucuna (Mucuna deeringiana) foliage meal. The animals were distributed according to a completely randomized design with 4 treatments and 3 replicates: control (maize-soybean) and 3 dietary mucuna foliage meal levels of inclusion: 50, 100 and 150 g kg -1. The apparent ileal digestibility of Crude Protein (CP) and 14 amino acids was determined.The protein digestibility was lower (p1 of mucuna inclusion than in the control diet. The coefficients of apparent ileal digestibility of amino acids in birds fed 150 g kg -1 mucuna foliage meal were lower (p-1 of mucuna inclusion in diets. No differences were observed between the control and 100g kg -1 mucuna inclusion diets for amino acid digestibilities except for lysine, aspartic acid and valine. It was discussed the role of the dietary fibre content and the presence of ...
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Physiology & behavior, Jan 2, 2015
Sheep have been suggested to use their senses to perceive plant properties and associate their in... more Sheep have been suggested to use their senses to perceive plant properties and associate their intake with consequences after ingestion. However, sheep with browsing experience do not seem to select against tannin-rich browsing materials in cafeteria trials. Thus, the objective of the present study was to evaluate the relationship between the chemical composition, selectivity index (SI), preference and intake rate (IR) of tannin-containing forage trees offered to sheep in cafeteria experiments. Four trees were selected for their condensed tannin content and their varying biological activities. Havardia albicans (high biological activity), Leucaena leucocephala (medium biological activity), Acacia gaumeri (low biological activity) and Brosimum alicastrum (very low biological activity) were used in this study. Ten hair sheep (23.7 kg±1.43 LW) with eight months of browsing experience in native vegetation were used in this study. Polyethylene glycol (PEG 3600MW) was administered to five...
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Veterinary Parasitology, 2008
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Veterinary Parasitology, 2008
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Veterinary Parasitology, 2010
Bioactive plants with anthelmintic (AH) properties represent a promising alternative solution to ... more Bioactive plants with anthelmintic (AH) properties represent a promising alternative solution to chemical treatments. The AH effect of several Mexican tannin-rich (TR) plants has been screened in vitro. The in vivo AH effect of one TR legume, Lysiloma latisiliquum (Tzalam) on nematode larval establishment was confirmed. The present trial aimed at evaluating the direct and indirect effects of L. latisiliquum fodder consumption on adult Haemonchus contortus. Twenty-two parasite-naïve hair sheep lambs were allocated to an infected group (I) (400H. contortus L(3)/kg BW on D0) and a non-infected group (NI). From D0 to D28 post infection (PI), all the lambs were fed a complete diet. On D28, the two groups were sub-divided into four groups. Two control (C) groups maintained on the original basal diet (CI: 6 infected lambs and CNI: 5 non-infected lambs). The two treatment groups (T) received L. latisiliquum fodder ad libitum up to D36 when lambs were humanely slaughtered (TI: 6 infected lambs and TNI: 5 non-infected lambs). From D28 to D36 PI, individual fodder consumption and nematode egg excretion were measured daily. At necropsy, abomasal contents were recovered to obtain worm burdens and measure the female worm length and fecundity. Histological samples were taken from the respective abomasums and small intestines to count mucosal inflammatory cells. An increased consumption of TR fodder was observed in the TI vs. the TNI group (P&amp;amp;amp;amp;lt;0.01). Before L. latisiliquum distribution, faecal egg excretion was similar in TI and CI groups. From D29 PI the TI group showed lower faecal egg counts compared to CI group (P&amp;amp;amp;amp;lt;0.02). Although no differences in worm burdens were observed, worms of the TI group were smaller and, according to their size, contained fewer eggs in utero than worms from the CI group (P&amp;amp;amp;amp;lt;0.05). Only minor differences in mucosal inflammatory cells were observed between groups, indicating that the indirect effect was not evident. Thus, a short-term consumption of L. latisiliquum can modulate directly the biology of adult H. contortus affecting the worm size and female fecundity while the worm burdens were not affected. Infected animals ate more L. latisiliquum fodder than non-infected animals.
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Small Ruminant Research, 2012
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Journal of Food Composition and Analysis, 2005
ABSTRACT Oyster mushrooms (Pleurotus ostreatus (Jacq.: Fr.) Kumm.) were grown in either maize or ... more ABSTRACT Oyster mushrooms (Pleurotus ostreatus (Jacq.: Fr.) Kumm.) were grown in either maize or pumpkin straw. Samples were taken for each one of the three harvests and analyzed for total nitrogen (N) content and amino acids profile. The substrate had no effect (P &gt; 0.05) on N content and amino acid profile of the fruits. However, N (g/100g DM) increased (P &lt; 0.05) from 4.13g in the first harvest to 5.74g in the third harvest. In general, the amino acids tended to be higher on the first harvest samples, but no changes were found (P &gt; 0.05) in the amino acid profile due to substrate or harvest, except for valine decreasing (P &lt; 0.05) from 3.96 to 3.15g/16g N. Changes in the N content of the fruit could be explained by changes in the stipe and pileus proportions as they had different N content (3.15 and 5.48 +/- 0.031 g N/100 g DM, respectively). The amino acid profile of the mushroom was adequate according to the FAO/WHO/UNU adult human amino acid requirements. (C) 2004 Published by Elsevier Inc.
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Experimental Parasitology, 2012
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animal, 2013
We evaluated the effect of feeding dietary tannins from Lysiloma latisiliquum fresh forage on the... more We evaluated the effect of feeding dietary tannins from Lysiloma latisiliquum fresh forage on the saliva tannin-binding capacity of hair sheep lambs without previous exposure to tannin-rich (TR) fodder. Twenty-four hair sheep lambs (13.6±3.04 kg LW) were fed a tannin-free diet at the beginning of the experimental period (from day 10 to 13). On day 14, lambs were distributed into three groups (n=8): control group (CG), fed with the tannin-free diet (from D10 to D112); tannin short-term group (TST), fed the basal diet and 650 g of L. latisiliquum forage (from D14 to D55); tannin long-term group (TLT), fed the basal diet and 650 g of L. latisiliquum forage (from D14 to D112). Saliva samples were collected from the mouth of each lamb in the morning before feeding time on D10 and D14 (baseline period), on D49 and D56 (period 1) and on D97 and D112 (period 2). The tannin binding response of salivary protein (∆% turbidity) was determined with the haze development test (HDT) using either tannic acid or L. latisiliquum forage acetone extract. A turbidity protein index (TPI) was calculated as (∆% turbidity/[salivary protein (mg)]). Differences in HDT and TPI in the different groups were compared by repeated measures ANOVA using Proc Mixed. All groups had similar ∆% turbidity throughout the experiment (P&amp;amp;gt;0.05). At baseline and period 1, the TPI of the different groups was similar (P&amp;amp;gt;0.05). On period 2 the TLT group showed higher TPI compared with CG (P&amp;amp;lt;0.05). Meanwhile, CG and TST showed similar salivary TPI. The saliva of hair sheep lambs consuming TR L. latisiliquum fresh fodder (TLT group) increased their TPI compared with control lambs not exposed to tannins.
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Animal Feed Science and Technology, 2012
ABSTRACT This study evaluated effects of Havardia albicans foliage intake on sheep dry matter dig... more ABSTRACT This study evaluated effects of Havardia albicans foliage intake on sheep dry matter digestibility (DMD) and included post-mortem evaluation of Haemonchus contortus infection (i.e., worm burdens, worm female length, fecundity). Fifteen hair sheep (16 ± 4.2 kg live weight (LW)) raised free of gastrointestinal nematodes were used. Sheep were fed a basal diet: grain based concentrate and Pennisetum purpureum grass (900 and 100 g/kg DM, respectively) and were infected on day 0 with 3000 H. contortus infective larvae. On day 28 post-infection (PI) sheep were distributed to three groups being: Control (100 g/kg DM of basal diet), HA group (basal diet 600 + H. albicans 400) and the HA + PEG group (basal diet 600 + H. albicans 400) + Polyethylene glycol (PEG). The H. albicans foliage was supplied for 13 d including 7 d of adaptation (i.e., day 28–40 PI). The content of condensed tannins (CT), total polyphenols (TP) and total tannins (TT) was determined in the foliage. Concentrate, grass and H. albicans intake (g DM/kg LW0.75), DMD and H. contortus egg excretions were determined. Sheep were slaughtered at day 41 PI to assess adult worm burdens, female worm lengths and fecundity (i.e., eggs in utero). Foliage had (g/kg DM): 71.5 CT, 61.3 TP and 66.9 TT. Experimental groups had similar feed intakes, and intake of H. albicans was not affected by PEG. The DMD of HA + PEG and HA groups was lower than Control (P&lt;0.05). Post-mortem worm burdens were similar in all groups, but the H. contortus females in the HA group were shorter and had reduced fecundity (P&lt;0.05). Adding PEG (HA + PEG) eliminated the effect of H. albicans intake on worm length. A short period of H. albicans intake affected the DMD of sheep and reduced the H. contortus female worm length and fecundity. The use of this type of tannin-rich foliage could bring nutritional and anti-parasitic benefits to the ruminants which consume them.
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Animal Feed Science and Technology, 2008
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Animal Feed Science and Technology, 2005
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INTERCIENCIA- …, 2006
Genaro Monforte-Braga. M.Sc. in Tropical Animal Production, Universidad Autónoma de Yucatán (UADY... more Genaro Monforte-Braga. M.Sc. in Tropical Animal Production, Universidad Autónoma de Yucatán (UADY), Mexico. Consultant in animal production, Yucatán, México. ... Concepción Capetillo-Leal. Biologist-Chemist and Diploma in Analytical Chemistry, UADY, Mexico. ...
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Acta Alimentaria, 2010
ABSTRACT Pleurotus djamor from Yucatan State, Mexico was compared with a local variety of P. ostr... more ABSTRACT Pleurotus djamor from Yucatan State, Mexico was compared with a local variety of P. ostreatus, in terms of chemical composition and amino acid profile. No differences were found (P=0.55) in crude protein content of P. djamor and P. ostreatus (20.2 +/- 0.15 vs. 20.3 +/- 0.16 g/100 g DM., respectively). P. ostreatus had a lower fiber and fat content (P&lt;0.05). No difference was found in ash content (P&gt;0.05). P. ostreatus has a higher proportion (P&lt;0.05) of valine, isoleucine, phenylalanine, aspartate and alanine while P. djamor had higher proportion (P&lt;0.05) of proline and glutamate, no difference was found in the remaining amino acids. Both species had a high proportion of aspartate and glutamate. In general, the human amino acid requirements for adults are satisfied, however, leucine and lysine might become limiting. It was concluded that P. djamor can be included in human diet in similar fashion as P. ostreatus.
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