The vacuolar protease B of Saccharomyces cerevisiae is a subtilisin-like protease encoded by the ... more The vacuolar protease B of Saccharomyces cerevisiae is a subtilisin-like protease encoded by the PRB1 gene. Antibodies raised against a synthetic peptide and an Escherichia coli-derived PRB1 open reading frame (ORF) protein cross-react with authentic protease B from yeast. By using these antibodies, the posttranslational biosynthetic pathway of protease B has been elucidated. Preproprotease B is a 76-kD unglycosylated precursor that enters the endoplasmic reticulum (ER), where it receives one asparagine-linked (Asn-linked) and an undetermined number of non-Asn-linked carbohydrate side chains. The large glycosylated intermediate is proteolytically processed to a 39-kD form before exiting the ER. In the Golgi complex, the 39-kD form becomes 40 kD, due to elaboration of the Asn-linked side chain. The carboxyterminal end of the 40-kD proprotease B undergoes protease A-mediated processing to a 37-kD intermediate, which in turn is quickly processed to 31-kD mature protease B. The ultimate...
International Journal of Managing Projects in Business, 2008
... Welcome: Google Crawler Account ... promise for helping to mature risk management in the phar... more ... Welcome: Google Crawler Account ... promise for helping to mature risk management in the pharmaceutical industry through selective, adaptive application. ... Pons, DJ, Raine, JK (2004), "Design with uncertain qualitative variables under imperfect knowledge", Proceedings of the ...
The vacuolar protease B of Saccharomyces cerevisiae is a subtilisin-like protease encoded by the ... more The vacuolar protease B of Saccharomyces cerevisiae is a subtilisin-like protease encoded by the PRB1 gene. Antibodies raised against a synthetic peptide and an Escherichia coli-derived PRB1 open reading frame (ORF) protein cross-react with authentic protease B from yeast. By using these antibodies, the posttranslational biosynthetic pathway of protease B has been elucidated. Preproprotease B is a 76-kD unglycosylated precursor that enters the endoplasmic reticulum (ER), where it receives one asparagine-linked (Asn-linked) and an undetermined number of non-Asn-linked carbohydrate side chains. The large glycosylated intermediate is proteolytically processed to a 39-kD form before exiting the ER. In the Golgi complex, the 39-kD form becomes 40 kD, due to elaboration of the Asn-linked side chain. The carboxyterminal end of the 40-kD proprotease B undergoes protease A-mediated processing to a 37-kD intermediate, which in turn is quickly processed to 31-kD mature protease B. The ultimate...
International Journal of Managing Projects in Business, 2008
... Welcome: Google Crawler Account ... promise for helping to mature risk management in the phar... more ... Welcome: Google Crawler Account ... promise for helping to mature risk management in the pharmaceutical industry through selective, adaptive application. ... Pons, DJ, Raine, JK (2004), "Design with uncertain qualitative variables under imperfect knowledge", Proceedings of the ...
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Papers by Colleen Dixon