Introduction: A group of metabolic diseases with a hyperglycemic phenotype known as diabetes mell... more Introduction: A group of metabolic diseases with a hyperglycemic phenotype known as diabetes mellitus impact both industrialized and developing nations. It is brought on by deviations in insulin synthesis, action, or both. Research Objective: This study seeks to associate the interaction of red cell indices and blood group in diabetic patients above 50 years of age. This is a case control study in which a convenient sample size of 100 individuals were recruited from a consenting population at a private hospital in Port Harcourt, Rivers State, 50 of whom were diabetic, and the remaining 50 were not. Methods: Using the automated 3-part differential hematology analyzer Sysmex KX-21N, a Full blood count (FBC) and differential was obtained using (Sysmex Corporation Kobe, Japan) Standardization, instrument calibration, and analysis of sample was achieved in line with accordance with the manufacturer's manual to determine the red cell indices and grouping of cells using the tile approa...
Hepatitis B virus infection is a serious global public health challenge that affects more than tw... more Hepatitis B virus infection is a serious global public health challenge that affects more than two billion people worldwide. This study aimed to evaluate the serological pattern of HBV infection in HBV infected patients in Port Harcourt, Nigeria. The main aim of this study was to evaluate the serological pattern of hepatitis B infection in Port Harcourt, Nigeria. This was a comparative cross sectional study carried out on 260 hepatitis B patients and blood donors attending hepatitis B clinics, and blood banks in Rivers State University Teaching Hospital, Port Harcourt, Military Hospital, Port Harcourt, and University of Port Harcourt Teaching Hospital, Choba. The study involved the use of hepatitis B panel assay, measurement of prevalence of hepatitis B virus infection in Port Harcourt, assessment of hepatitis B serological markers in all subjects, determination of the presence and prevalence of occult HBV among participants. HBV 5-parameter (panel) Rapid Test kit was used to assess...
International Journal of TROPICAL DISEASE & Health, 2021
Background: Occult hepatitis B infection (OBI) is a rare form of infection that is characterized ... more Background: Occult hepatitis B infection (OBI) is a rare form of infection that is characterized by the presence of replication-competent HBV DNA in the liver but without detectable HBsAg in the serum. Aim: This study aimed to determine the comparative levels of immunological variables particularly CD4 counts and differential white blood cell (WBC) counts in occult hepatitis B and HBsAg positive subjects among prospective blood donors in Port Harcourt Metropolis in Nigeria. Methods: The CD4 count and total and differential WBC counts were analyzed with automated techniques using BD Fascount and Sysmex XP-300 respectively. Data were analyzed as mean (standard deviation) and significance was assumed at 95% confidence interval using student t-test and one-way ANOVA on GraphPad prism. Results: The mean values for CD4, WBC, LYM (lymphocyte), MXD (differential mixed cells), and NEU (neutrophils) were 607±286 µ/l, 16±24 /l, 1.8±0.55 %, 0.46±0.15 % and 3.1±1.1 % respectively for occult hepa...
Journal of Pharmaceutical Research International, 2020
The was a case control and comparative study aimed at investigating the possible effects of injec... more The was a case control and comparative study aimed at investigating the possible effects of injectable and skin patch contraceptives on selected haematologic and haemostatic parameters in women attending primary healthcare centre at Eleme, Rivers State The study population consists of seventy-five (75) apparently healthy, non-pregnant, non- smoking women, aged between 25 and 45years; (31 women on DEPO-PROVERA contraceptive, 14 women on Implanon, and 30 apparently healthy non-contraceptive users as control group). Fibrinogen, antithrombin, tissue plasminogen activator was analysed with reagents prepared by Elabscience, Wuhan, China, using an ELISA machine (STAT FAX-2100). Prothrombin time and activated partial thromboplastin time was done manually with reagents prepared by Quimica Clinica Aplicada S.A, Spain. Haematologic parameters were analysed using SYSMEX KX-21-N auto analyser. Graph-pad Prism 5.0 was used in analysing all data, p < 0.05 was considered statistically significa...
summary. Anti‐HCV pre‐transfusion testing among blood donors has not been introduced as a mandat... more summary. Anti‐HCV pre‐transfusion testing among blood donors has not been introduced as a mandatory test in Rivers State, hence the risk of transfusion‐transmitted HCV cannot be fully ascertained. One thousand (1000) apparently healthy blood donors were screened using a rapid second – generation test, the HEP C SPOT HCV assay. An overall prevalence of 2.9% was observed in this study. The highest prevalence (8.1%) was found among adults aged between 26 and 33 years and commercial donors.
ABSTRACT An enzyme immunoassay was used to detect antibodies to hepatitis C virus (anti-HCV) in 2... more ABSTRACT An enzyme immunoassay was used to detect antibodies to hepatitis C virus (anti-HCV) in 261 patients and 69 staff members of a hemodialysis unit. The prevalence of anti-HCV was 46.7% in patients and 2.9% in staff members (p less than 0.001). The prevalence of anti-HCV increased significantly with increasing duration of hemodialysis (p less than 0.001), but was not related to age, sex, history of blood transfusion, status of hepatitis B or hepatitis A virus infection, or serum ALT. Patients with hepatitis episode increased with increasing duration of hemodialysis and showed a significantly higher prevalence of anti-HCV than those without (63.1 vs. 34.7%, p less than 0.001). The prevalence of anti-HCV in patients with hepatitis also increased with increasing duration of hemodialysis (p = 0.05). Thus, HCV appears to be the major cause of hepatitis in hemodialysis patients. Besides strict infection control measures, further studies are needed to determine the mode of HCV infection and its prevention in the hemodialysis unit.
In a setting where there is no functional blood transfusion service, there is a need to establish... more In a setting where there is no functional blood transfusion service, there is a need to establish reference values which will assist in determining the cutoff values of the donor pool and also to evaluate anemia. Three hundred and forty eight blood donors of both sexes, aged 18 to 62 years were studied in a cross-sectional descriptive study. Four biochemical parameters: serum ferritin (SF), serum iron (SI), total iron binding capacity (TIBC) and transferrin saturation (TS) and four hematological parameters: hemoglobin (Hb), packed cell volume (PCV), total white blood cell count (WBC) and erythrocyte sedimentation rate (ESR), were assessed. The median and percentile ranges (10-90% percentile) of the biochemical and hematological parameters were as follows: SF 46. 8 ng/ml (0. 0-173. 1), SI 85. 9 mg/dl (19. 7-338. 0), TIBC 224. 7 mg/dl (60. 9-541. 4), TS 41. 2% (15. 3-90. 6), Hb 12. 9 g/dl (7. 74-15. 6), PCV 38. 0% (22. 9-47. 0), WBC 4. 5610 9 /l (3. 0-8. 0) and ESR 8. 0 mm/h (1. 0-24. 6). The sex of the blood donor volunteers was found to exert an influence on SF, SI, TIBC and WBC in favor of the female; P,0. 001. The age of the blood donors had a significant influence on some of the variables especially the hematological variables such as Hb, PCV and ESR (P,0. 001). At a cutoff value of Hb 12. 0 g/dl, SF 15. 0 ng/ml and TS 16, 10. 4 and 6. 0% had iron deficiency and iron deficiency anemia respectively in this study population. There is a high percentage of iron deficiency anemia in this donor population and this calls for urgent attention.
Introduction: A group of metabolic diseases with a hyperglycemic phenotype known as diabetes mell... more Introduction: A group of metabolic diseases with a hyperglycemic phenotype known as diabetes mellitus impact both industrialized and developing nations. It is brought on by deviations in insulin synthesis, action, or both. Research Objective: This study seeks to associate the interaction of red cell indices and blood group in diabetic patients above 50 years of age. This is a case control study in which a convenient sample size of 100 individuals were recruited from a consenting population at a private hospital in Port Harcourt, Rivers State, 50 of whom were diabetic, and the remaining 50 were not. Methods: Using the automated 3-part differential hematology analyzer Sysmex KX-21N, a Full blood count (FBC) and differential was obtained using (Sysmex Corporation Kobe, Japan) Standardization, instrument calibration, and analysis of sample was achieved in line with accordance with the manufacturer's manual to determine the red cell indices and grouping of cells using the tile approa...
Hepatitis B virus infection is a serious global public health challenge that affects more than tw... more Hepatitis B virus infection is a serious global public health challenge that affects more than two billion people worldwide. This study aimed to evaluate the serological pattern of HBV infection in HBV infected patients in Port Harcourt, Nigeria. The main aim of this study was to evaluate the serological pattern of hepatitis B infection in Port Harcourt, Nigeria. This was a comparative cross sectional study carried out on 260 hepatitis B patients and blood donors attending hepatitis B clinics, and blood banks in Rivers State University Teaching Hospital, Port Harcourt, Military Hospital, Port Harcourt, and University of Port Harcourt Teaching Hospital, Choba. The study involved the use of hepatitis B panel assay, measurement of prevalence of hepatitis B virus infection in Port Harcourt, assessment of hepatitis B serological markers in all subjects, determination of the presence and prevalence of occult HBV among participants. HBV 5-parameter (panel) Rapid Test kit was used to assess...
International Journal of TROPICAL DISEASE & Health, 2021
Background: Occult hepatitis B infection (OBI) is a rare form of infection that is characterized ... more Background: Occult hepatitis B infection (OBI) is a rare form of infection that is characterized by the presence of replication-competent HBV DNA in the liver but without detectable HBsAg in the serum. Aim: This study aimed to determine the comparative levels of immunological variables particularly CD4 counts and differential white blood cell (WBC) counts in occult hepatitis B and HBsAg positive subjects among prospective blood donors in Port Harcourt Metropolis in Nigeria. Methods: The CD4 count and total and differential WBC counts were analyzed with automated techniques using BD Fascount and Sysmex XP-300 respectively. Data were analyzed as mean (standard deviation) and significance was assumed at 95% confidence interval using student t-test and one-way ANOVA on GraphPad prism. Results: The mean values for CD4, WBC, LYM (lymphocyte), MXD (differential mixed cells), and NEU (neutrophils) were 607±286 µ/l, 16±24 /l, 1.8±0.55 %, 0.46±0.15 % and 3.1±1.1 % respectively for occult hepa...
Journal of Pharmaceutical Research International, 2020
The was a case control and comparative study aimed at investigating the possible effects of injec... more The was a case control and comparative study aimed at investigating the possible effects of injectable and skin patch contraceptives on selected haematologic and haemostatic parameters in women attending primary healthcare centre at Eleme, Rivers State The study population consists of seventy-five (75) apparently healthy, non-pregnant, non- smoking women, aged between 25 and 45years; (31 women on DEPO-PROVERA contraceptive, 14 women on Implanon, and 30 apparently healthy non-contraceptive users as control group). Fibrinogen, antithrombin, tissue plasminogen activator was analysed with reagents prepared by Elabscience, Wuhan, China, using an ELISA machine (STAT FAX-2100). Prothrombin time and activated partial thromboplastin time was done manually with reagents prepared by Quimica Clinica Aplicada S.A, Spain. Haematologic parameters were analysed using SYSMEX KX-21-N auto analyser. Graph-pad Prism 5.0 was used in analysing all data, p < 0.05 was considered statistically significa...
summary. Anti‐HCV pre‐transfusion testing among blood donors has not been introduced as a mandat... more summary. Anti‐HCV pre‐transfusion testing among blood donors has not been introduced as a mandatory test in Rivers State, hence the risk of transfusion‐transmitted HCV cannot be fully ascertained. One thousand (1000) apparently healthy blood donors were screened using a rapid second – generation test, the HEP C SPOT HCV assay. An overall prevalence of 2.9% was observed in this study. The highest prevalence (8.1%) was found among adults aged between 26 and 33 years and commercial donors.
ABSTRACT An enzyme immunoassay was used to detect antibodies to hepatitis C virus (anti-HCV) in 2... more ABSTRACT An enzyme immunoassay was used to detect antibodies to hepatitis C virus (anti-HCV) in 261 patients and 69 staff members of a hemodialysis unit. The prevalence of anti-HCV was 46.7% in patients and 2.9% in staff members (p less than 0.001). The prevalence of anti-HCV increased significantly with increasing duration of hemodialysis (p less than 0.001), but was not related to age, sex, history of blood transfusion, status of hepatitis B or hepatitis A virus infection, or serum ALT. Patients with hepatitis episode increased with increasing duration of hemodialysis and showed a significantly higher prevalence of anti-HCV than those without (63.1 vs. 34.7%, p less than 0.001). The prevalence of anti-HCV in patients with hepatitis also increased with increasing duration of hemodialysis (p = 0.05). Thus, HCV appears to be the major cause of hepatitis in hemodialysis patients. Besides strict infection control measures, further studies are needed to determine the mode of HCV infection and its prevention in the hemodialysis unit.
In a setting where there is no functional blood transfusion service, there is a need to establish... more In a setting where there is no functional blood transfusion service, there is a need to establish reference values which will assist in determining the cutoff values of the donor pool and also to evaluate anemia. Three hundred and forty eight blood donors of both sexes, aged 18 to 62 years were studied in a cross-sectional descriptive study. Four biochemical parameters: serum ferritin (SF), serum iron (SI), total iron binding capacity (TIBC) and transferrin saturation (TS) and four hematological parameters: hemoglobin (Hb), packed cell volume (PCV), total white blood cell count (WBC) and erythrocyte sedimentation rate (ESR), were assessed. The median and percentile ranges (10-90% percentile) of the biochemical and hematological parameters were as follows: SF 46. 8 ng/ml (0. 0-173. 1), SI 85. 9 mg/dl (19. 7-338. 0), TIBC 224. 7 mg/dl (60. 9-541. 4), TS 41. 2% (15. 3-90. 6), Hb 12. 9 g/dl (7. 74-15. 6), PCV 38. 0% (22. 9-47. 0), WBC 4. 5610 9 /l (3. 0-8. 0) and ESR 8. 0 mm/h (1. 0-24. 6). The sex of the blood donor volunteers was found to exert an influence on SF, SI, TIBC and WBC in favor of the female; P,0. 001. The age of the blood donors had a significant influence on some of the variables especially the hematological variables such as Hb, PCV and ESR (P,0. 001). At a cutoff value of Hb 12. 0 g/dl, SF 15. 0 ng/ml and TS 16, 10. 4 and 6. 0% had iron deficiency and iron deficiency anemia respectively in this study population. There is a high percentage of iron deficiency anemia in this donor population and this calls for urgent attention.
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