This study aimed to determine whether preemptive fentanyl administration in neonatal rats reduces... more This study aimed to determine whether preemptive fentanyl administration in neonatal rats reduces the impact of a nociceptive stimulus initiated during the first day of life (P1) on hippocampal neurogenesis, behavior, and learning. At P1, Wistar rat pups received either a subcutaneous injection of fentanyl (F) before intraplantar injection of complete Freund’s adjuvant (CFA) (CFA + F group), an isolated injection of CFA (CFA group), or subcutaneous injection of fentanyl without CFA injection (F). Control animals received saline injections using the same route and volume as the treatment groups. Hippocampal neurogenesis was evaluated by 5′ –bromo-2′-deoxyuridine (BrdU) staining on P10 and P39 to assess neuronal proliferation and survival, respectively. Anxiety behavior in adulthood was assessed using an open field test (OF) and an elevated plus maze test (EPM). Spatial memory was assessed on a Morris water maze test (MWM), where the animals were trained for seven days, beginning on P...
International Journal of Developmental Neuroscience, 2021
Maternal separation and neonatal manipulation of pups produce changes in maternal behavior after ... more Maternal separation and neonatal manipulation of pups produce changes in maternal behavior after the dam–pup reunion. Here, we examined whether continuous versus alternating days of neonatal manipulation during the first 8 postnatal days produces differential changes in maternal and non‐maternal behaviors in rats. We found that both maternal separation protocols increased anogenital licking after dam–pup reunion, reflecting increased maternal care of pups.
The difficulty in assessing pain during the neonatal period is one of the main obstacles for appr... more The difficulty in assessing pain during the neonatal period is one of the main obstacles for appropriate analgesia in intensive care units. The aim of this study was to develop and validate computer software to monitor neonatal facial movements of pain in real time. The software was developed in the Delphi integrated development environment and provides real-time image analysis during monitoring, based on image recognition of pain-related facial actions. To validate the software performance, facial images were obtained during the monitoring of 30 neonates who were subjected to painful procedures related to daily care management. Of the 5644 images identified and analysed by the software, 360 images - 12 per infant - were randomly selected and assessed by six healthcare professionals with experience of recognising neonatal pain. The agreement between the examiners and the software assessment was excellent (κ = 0.975). The software exhibited 85% sensitivity and 100% specificity in detecting neutral facial expressions in the resting state and 100% sensitivity and specificity in detecting pain during painful procedures. It is possible to assess neonatal procedural pain using computer software that has good sensitivity and specificity to detect facial movements.
to describe the epidemiological data of the population born with the diagnosis of Congenital Hear... more to describe the epidemiological data of the population born with the diagnosis of Congenital Heart Disease (CHD); to compare diagnoses made using fetal echocardiography with the findings from postnatal echocardiography or anatomopathological examination of the heart; and to evaluate mortality among newborns that underwent surgical treatment. this was a cohort study with information gathered from the medical records of the pregnant women and their newborns diagnosed with CHD during the fetal or postnatal periods, between January 2008 and December 2012. Means, standard deviations and maximum and minimum values were calculated for the quantitative variables. Relative and absolute values were calculated for the qualitative variables. The heart malformations were categorized in four groups: complex lesions, significant lesions, minor lesions and others. we detected postnatal incidence of CHD of 1.9% at our service. The mean maternal age was 28.3 years and 10 (21.3%) of the pregnant women...
Journal of Maternal-Fetal and Neonatal Medicine, 2014
Abstract Objective: To evaluate lung maturity using ultrasound (US), comparing the subjective and... more Abstract Objective: To evaluate lung maturity using ultrasound (US), comparing the subjective and gray-scale histogram (GSH) techniques. Methods: A total of 77 single pregnancies were evaluated and divided into the following two groups: 11 women of gestational age 28 to 35 + 6 weeks and 66 women ≥ 36 weeks. The women underwent to emergency or planned cesarean section, according to fetal-maternal indications. The US was performed on the mean sagittal plane of the fetal torso, in order to observe the lung and hepatic areas. Fetal lung maturity was evaluated subjectively and through GSH. After delivery, the incidence of respiratory distress in the newborn was evaluated. The analyses were considered to be correct or incorrect, and comparisons were made using the McNemar test. In order to compare lung/hepatic echogenicity using GSH in groups with and without respiratory distress, the Student's t-test was used. Results: The subjective evaluation identified 41 cases (53.2%) correctly and 36 (46.8%) incorrectly, while GSH found 58 (75.3%) correctly and 19 (24.7%) incorrectly (p = 0.006). There was a significant difference in mean lung/hepatic echogenicity between the groups with and without respiratory distress (1.05 versus 1.26; p = 0.002). In the group of 28 to 35 + 6 weeks, GSH presented sensitivity, specificity and accuracy in predicting respiratory distress of 61.9%, 89.1% and 81.6%, respectively. Conclusion: The evaluation of fetal lung maturity through GSH was more effective than the subjective method in predicting respiratory distress among newborns.
This study aimed to determine whether preemptive fentanyl administration in neonatal rats reduces... more This study aimed to determine whether preemptive fentanyl administration in neonatal rats reduces the impact of a nociceptive stimulus initiated during the first day of life (P1) on hippocampal neurogenesis, behavior, and learning. At P1, Wistar rat pups received either a subcutaneous injection of fentanyl (F) before intraplantar injection of complete Freund’s adjuvant (CFA) (CFA + F group), an isolated injection of CFA (CFA group), or subcutaneous injection of fentanyl without CFA injection (F). Control animals received saline injections using the same route and volume as the treatment groups. Hippocampal neurogenesis was evaluated by 5′ –bromo-2′-deoxyuridine (BrdU) staining on P10 and P39 to assess neuronal proliferation and survival, respectively. Anxiety behavior in adulthood was assessed using an open field test (OF) and an elevated plus maze test (EPM). Spatial memory was assessed on a Morris water maze test (MWM), where the animals were trained for seven days, beginning on P...
International Journal of Developmental Neuroscience, 2021
Maternal separation and neonatal manipulation of pups produce changes in maternal behavior after ... more Maternal separation and neonatal manipulation of pups produce changes in maternal behavior after the dam–pup reunion. Here, we examined whether continuous versus alternating days of neonatal manipulation during the first 8 postnatal days produces differential changes in maternal and non‐maternal behaviors in rats. We found that both maternal separation protocols increased anogenital licking after dam–pup reunion, reflecting increased maternal care of pups.
The difficulty in assessing pain during the neonatal period is one of the main obstacles for appr... more The difficulty in assessing pain during the neonatal period is one of the main obstacles for appropriate analgesia in intensive care units. The aim of this study was to develop and validate computer software to monitor neonatal facial movements of pain in real time. The software was developed in the Delphi integrated development environment and provides real-time image analysis during monitoring, based on image recognition of pain-related facial actions. To validate the software performance, facial images were obtained during the monitoring of 30 neonates who were subjected to painful procedures related to daily care management. Of the 5644 images identified and analysed by the software, 360 images - 12 per infant - were randomly selected and assessed by six healthcare professionals with experience of recognising neonatal pain. The agreement between the examiners and the software assessment was excellent (κ = 0.975). The software exhibited 85% sensitivity and 100% specificity in detecting neutral facial expressions in the resting state and 100% sensitivity and specificity in detecting pain during painful procedures. It is possible to assess neonatal procedural pain using computer software that has good sensitivity and specificity to detect facial movements.
to describe the epidemiological data of the population born with the diagnosis of Congenital Hear... more to describe the epidemiological data of the population born with the diagnosis of Congenital Heart Disease (CHD); to compare diagnoses made using fetal echocardiography with the findings from postnatal echocardiography or anatomopathological examination of the heart; and to evaluate mortality among newborns that underwent surgical treatment. this was a cohort study with information gathered from the medical records of the pregnant women and their newborns diagnosed with CHD during the fetal or postnatal periods, between January 2008 and December 2012. Means, standard deviations and maximum and minimum values were calculated for the quantitative variables. Relative and absolute values were calculated for the qualitative variables. The heart malformations were categorized in four groups: complex lesions, significant lesions, minor lesions and others. we detected postnatal incidence of CHD of 1.9% at our service. The mean maternal age was 28.3 years and 10 (21.3%) of the pregnant women...
Journal of Maternal-Fetal and Neonatal Medicine, 2014
Abstract Objective: To evaluate lung maturity using ultrasound (US), comparing the subjective and... more Abstract Objective: To evaluate lung maturity using ultrasound (US), comparing the subjective and gray-scale histogram (GSH) techniques. Methods: A total of 77 single pregnancies were evaluated and divided into the following two groups: 11 women of gestational age 28 to 35 + 6 weeks and 66 women ≥ 36 weeks. The women underwent to emergency or planned cesarean section, according to fetal-maternal indications. The US was performed on the mean sagittal plane of the fetal torso, in order to observe the lung and hepatic areas. Fetal lung maturity was evaluated subjectively and through GSH. After delivery, the incidence of respiratory distress in the newborn was evaluated. The analyses were considered to be correct or incorrect, and comparisons were made using the McNemar test. In order to compare lung/hepatic echogenicity using GSH in groups with and without respiratory distress, the Student's t-test was used. Results: The subjective evaluation identified 41 cases (53.2%) correctly and 36 (46.8%) incorrectly, while GSH found 58 (75.3%) correctly and 19 (24.7%) incorrectly (p = 0.006). There was a significant difference in mean lung/hepatic echogenicity between the groups with and without respiratory distress (1.05 versus 1.26; p = 0.002). In the group of 28 to 35 + 6 weeks, GSH presented sensitivity, specificity and accuracy in predicting respiratory distress of 61.9%, 89.1% and 81.6%, respectively. Conclusion: The evaluation of fetal lung maturity through GSH was more effective than the subjective method in predicting respiratory distress among newborns.
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