Introduction: In the present study we investigated the performance, precision, and recovery of th... more Introduction: In the present study we investigated the performance, precision, and recovery of three different automated methods in determining cancer antigen (CA) 15-3 levels. Methods: Serum samples were obtained from 60 hospitalized female patients. As controls, commercially available samples were used. Cancer antigen (CA) 15-3 levels were measured using ARCHITECT CA 15-3, Elecsys® CA 15-3, and Vitros CA 15-3 immunoassays. A comparison of the results between the three methods was conducted, and the precision and recovery were analyzed. Results: Coefficient of variations (CVs), determined with low- and high-level-CA 15-3 control samples, and reproducibility values were: 2.56-2.80% and 3.10-4. 20% for ARCHITECT i2000SR immunoassay analyzer; 3.50-5.55% and 4.88-6.47% for Cobas E 601 analyzer; 3.30-4.0% and 4.30-4.80% for VITROS 5600 Integrated System, respectively. The percent recoveries were 95-98% for Elecsys® CA 15-3 assay, 93-105% for Vitros CA 15-3 assay, and 92-95% for ARCHITEC...
Introduction Today we have a large number of articles that investigate oxidative stress and poten... more Introduction Today we have a large number of articles that investigate oxidative stress and potential role of nitric oxide (NO) in the pathophysiology of schizophrenia and a lot of evidence of altered antioxidant capacity. Interesting scientifically facts is that bilirubin acts as an endogenous scavenger of NO, giving him the role of antioxidant that is reduced in schizophrenia. Objectives Antioxidant properties of bilirubin through its interaction with the nitric propose that bilirubin-nitric oxide as a new biomarker of oxidative / nitrosative stress. Aims The aim of our study was to investigate possible correlation between serum levels of nitric oxide and bilirubin in patient's whit schizophrenia. Methods The study was consisted of 50 patients with schizophrenia and 50 healthy controls. We investigated the levels of nitric oxide, which is determined by conversion of nitrate to nitrite and then measuring with Greiss reagent, but only in group patients we measured the mean levels of bilirubin, within of course of illness. Results Mean of NO between group patients and controls are statistically significant (CI = 13.31–27.29, t = 5.863, p = 0.0001). The average value of total bilirubin in patients suffering from schizophrenia was presented with SD = 11.77; 6.06 ± 0.86, and is statistically significant differences between the flow of diseases with the level of bilirubin, where the highest values detected markedly with the first hospitalization. Conclusion Antioxidant capacity of schizophrenia decreases with the progress of the disease. We can deduce that imbalanced between NO and bilirubin participates in the pathogenesis basis of schizophrenia.
Schizophrenia is a severe mental disorder with relatively high occurrence in general population (... more Schizophrenia is a severe mental disorder with relatively high occurrence in general population (around 1%). Classical genetic studies (Family, twin and adoption) undoubtedly prove its genetic etiology. On the other hand genetics studies are important in exploration of innate mechanisms responsible for specific response to antipsychotic medications. Significant differences in therapeutic responses to antipsychotic agents in patients suffering from schizophrenia were observed and therefore pharmacogenetics of antipsychotics represents main focus in current psychiatric genetics studies.This is particularly observed at polymorphisms in dopamine and serotonin receptor genes, but also some other biomolecules involved in signal transduction mechanism such are regulators of G-protein signalling in brain (RGS4). In order to test this hypothesis, association between certain variants of RGS4 linked polymorhic marker and responsiveness status towards antypsychotics was evaluated within the gro...
ObjectivesThere is a growing evidence that oxidative injury contributes to the patophysiology of ... more ObjectivesThere is a growing evidence that oxidative injury contributes to the patophysiology of schizophrenia (Sch), where higher concentrations of nitric oxide (NO) found to be neurotoxic. Data also suggest that bilirubin (BR) can serve as an endogenous scavenger of NO. The aim of this pilot study was to compare the serum levels of BR and NO among patients suffering from Sch, as well as to estimate whether NO serum levels differ between patients and healthy controls.MethodsThe study population were consisted of inpatients (n=20) who met DSM-IV diagnostic criteria for Sch confirmed by Structured Clinical Interview (SCID 1) and healthy controls (n=20). In order to exclude psychiatric morbidity in control subjects the same diagnostic procedure was applied. Serum BR levels were measured by the method of Ehrlich. NO concentration in serum was determined by classic colorimetric Griess reactionResultsIn patients with schizophrenia BR serum level were 9.85±1.182 mmol/L ; X±SEM. Serum NO l...
Introduction: In the present study we investigated the performance, precision, and recovery of th... more Introduction: In the present study we investigated the performance, precision, and recovery of three different automated methods in determining cancer antigen (CA) 15-3 levels. Methods: Serum samples were obtained from 60 hospitalized female patients. As controls, commercially available samples were used. Cancer antigen (CA) 15-3 levels were measured using ARCHITECT CA 15-3, Elecsys® CA 15-3, and Vitros CA 15-3 immunoassays. A comparison of the results between the three methods was conducted, and the precision and recovery were analyzed. Results: Coefficient of variations (CVs), determined with low- and high-level-CA 15-3 control samples, and reproducibility values were: 2.56-2.80% and 3.10-4. 20% for ARCHITECT i2000SR immunoassay analyzer; 3.50-5.55% and 4.88-6.47% for Cobas E 601 analyzer; 3.30-4.0% and 4.30-4.80% for VITROS 5600 Integrated System, respectively. The percent recoveries were 95-98% for Elecsys® CA 15-3 assay, 93-105% for Vitros CA 15-3 assay, and 92-95% for ARCHITEC...
Introduction Today we have a large number of articles that investigate oxidative stress and poten... more Introduction Today we have a large number of articles that investigate oxidative stress and potential role of nitric oxide (NO) in the pathophysiology of schizophrenia and a lot of evidence of altered antioxidant capacity. Interesting scientifically facts is that bilirubin acts as an endogenous scavenger of NO, giving him the role of antioxidant that is reduced in schizophrenia. Objectives Antioxidant properties of bilirubin through its interaction with the nitric propose that bilirubin-nitric oxide as a new biomarker of oxidative / nitrosative stress. Aims The aim of our study was to investigate possible correlation between serum levels of nitric oxide and bilirubin in patient's whit schizophrenia. Methods The study was consisted of 50 patients with schizophrenia and 50 healthy controls. We investigated the levels of nitric oxide, which is determined by conversion of nitrate to nitrite and then measuring with Greiss reagent, but only in group patients we measured the mean levels of bilirubin, within of course of illness. Results Mean of NO between group patients and controls are statistically significant (CI = 13.31–27.29, t = 5.863, p = 0.0001). The average value of total bilirubin in patients suffering from schizophrenia was presented with SD = 11.77; 6.06 ± 0.86, and is statistically significant differences between the flow of diseases with the level of bilirubin, where the highest values detected markedly with the first hospitalization. Conclusion Antioxidant capacity of schizophrenia decreases with the progress of the disease. We can deduce that imbalanced between NO and bilirubin participates in the pathogenesis basis of schizophrenia.
Schizophrenia is a severe mental disorder with relatively high occurrence in general population (... more Schizophrenia is a severe mental disorder with relatively high occurrence in general population (around 1%). Classical genetic studies (Family, twin and adoption) undoubtedly prove its genetic etiology. On the other hand genetics studies are important in exploration of innate mechanisms responsible for specific response to antipsychotic medications. Significant differences in therapeutic responses to antipsychotic agents in patients suffering from schizophrenia were observed and therefore pharmacogenetics of antipsychotics represents main focus in current psychiatric genetics studies.This is particularly observed at polymorphisms in dopamine and serotonin receptor genes, but also some other biomolecules involved in signal transduction mechanism such are regulators of G-protein signalling in brain (RGS4). In order to test this hypothesis, association between certain variants of RGS4 linked polymorhic marker and responsiveness status towards antypsychotics was evaluated within the gro...
ObjectivesThere is a growing evidence that oxidative injury contributes to the patophysiology of ... more ObjectivesThere is a growing evidence that oxidative injury contributes to the patophysiology of schizophrenia (Sch), where higher concentrations of nitric oxide (NO) found to be neurotoxic. Data also suggest that bilirubin (BR) can serve as an endogenous scavenger of NO. The aim of this pilot study was to compare the serum levels of BR and NO among patients suffering from Sch, as well as to estimate whether NO serum levels differ between patients and healthy controls.MethodsThe study population were consisted of inpatients (n=20) who met DSM-IV diagnostic criteria for Sch confirmed by Structured Clinical Interview (SCID 1) and healthy controls (n=20). In order to exclude psychiatric morbidity in control subjects the same diagnostic procedure was applied. Serum BR levels were measured by the method of Ehrlich. NO concentration in serum was determined by classic colorimetric Griess reactionResultsIn patients with schizophrenia BR serum level were 9.85±1.182 mmol/L ; X±SEM. Serum NO l...
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