Four major compounds were separated and identified from the methanol extracts of Nerium indicum flowers (Arali) using HPLC and mass spectral data. Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid... more
Four major compounds were separated and identified from the methanol extracts of Nerium indicum flowers (Arali) using HPLC and mass spectral data. Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid (1), quercetin-3-O- rutinoside (2), luteolin-5-O-rutinoside (3) and luteolin-7-O-rutinoside (4). In addition, the cis isomers of 5-O-caffeoylquinic acid in Nerium indicum flowers were confirmed by Mass, HPLC and UV. The structures of these compounds confirmed with the help of liquid chromatography mass spectrometry.
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Doxorubicin (DOX) is one of the most used chemotherapeutic agents in the treatment of various types of cancer. However, a continual problem that is associated with its application in therapeutic regimens is the development of... more
Doxorubicin (DOX) is one of the most used chemotherapeutic agents in the treatment of various types of cancer. However, a continual problem that is associated with its application in therapeutic regimens is the development of dose-dependent cardiotoxicity. The progression of this process is associated with a range of different mechanisms, but especially with the high level of oxidative stress. The aim of the study was to evaluate the effects of the water and methanol–water extracts from the plant Centaurea castriferrei (CAS) obtained by the ultrasound-assisted extraction method on the DOX-induced cardiotoxicity in the rat embryonic cardiomyocyte cell line H9c2. The H9c2 cells were treated for 48 h with the DOX and water or methanol–water extracts, or a combination (DOX + CAS H2O/CAS MeOH). The MTT assay, cell cycle analysis, and apoptosis detection revealed that both the tested extracts significantly abolished the cytotoxic effect caused by DOX. Moreover, the detection of oxidative ...
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The Centaurea L. (Asteraceae) genus includes many plant species with therapeutic properties. Centaurea castriferrei Borbás & Waisb is one of the least known and least described plants of this genus. The aim of the study was the... more
The Centaurea L. (Asteraceae) genus includes many plant species with therapeutic properties. Centaurea castriferrei Borbás & Waisb is one of the least known and least described plants of this genus. The aim of the study was the phytochemical analysis of water and methanol–water extracts (7:3 v/v) obtained from the aerial parts of the plant as well as evaluation of their anticancer activity. Quantitative determinations of phenolic compounds and flavonoids were performed, and the antioxidant potential was measured using the CUPRAC method. The RP-HPLC/DAD analysis and HPLC-ESI-QTOF-MS mass spectroscopy were performed, to determine the extracts’ composition. The antiproliferative activity of the obtained extracts was tested in thirteen cancer cell lines and normal skin fibroblasts using MTT test. Regardless of the extraction method and the extractant used, similar cytotoxicity of the extracts on most cancer cell lines was observed. However, the methanol–water extracts (7:3 v/v) containe...
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The composition of the ethanolic extract from the aerial parts of Crocus alatavicus Regel & Semen from southern Kazakhstan spontaneous flora was analyzed together with the determination of its antibacterial, antifungal, antiviral and... more
The composition of the ethanolic extract from the aerial parts of Crocus alatavicus Regel & Semen from southern Kazakhstan spontaneous flora was analyzed together with the determination of its antibacterial, antifungal, antiviral and anticancer activity. The phytochemical profile analysis by high-performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (HPLC/ESI-QTOF-MS) revealed the presence of multiple kaempferol derivatives. High-performance reverse-phase liquid chromatography combined with a photodiode-array detection (RP-HPLC/PDA) found that kaempferol 3-O-dihexoside and kaempferol 3-O-acyltetrahexoside accounted for 70.5% of the kaempferol derivatives. The minimum inhibitory concentration (MIC) values of the extract for all the tested reference microorganisms were high, reaching 10 mg/mL for yeasts and 20 mg/mL for bacteria. In contrast, antiviral activity was observed at 2 mg/mL, resulting in the inhibition of the HSV-1-induced cyt...
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Additional file 2: Figure S2. HPLC-DAD chromatogram (λ = 325 nm) - ethanolic extract from A. gmelinii Weber ex Stechm.
Research Interests: Chemistry, Analytical Chemistry, Medicine, Sample Preparation, Quantitative analysis, and 12 moreSolid Phase Extraction, Paclitaxel, Trees, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Plant extracts, Quantitative Analysis, Plant Extract, Quntitative Thin Layer Chromatography, Elsevier, Thin Layer Chromatography, and Pharmacology and pharmaceutical sciences
ABSTRACT The qualitative and quantitative analysis of phenolic acids was carried out using methods of HPLC with the diode array detector (DAD). The quantitative analysis of chosen phenolic acids was carried out using the Multicomponent... more
ABSTRACT The qualitative and quantitative analysis of phenolic acids was carried out using methods of HPLC with the diode array detector (DAD). The quantitative analysis of chosen phenolic acids was carried out using the Multicomponent Analysis method (MCA). Solid-phase extraction was applied for the purification of crude extract from aerial part of three species of fam. Asteraceae (Chrysanthemum maximum Ram.(DC), Chrysanthemum segetum L., Rudbeckia laciniata L.). The choice of optimal conditions of analysis was made using DryLab+ software.
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Additional file 1: Figure S1. HPLC-DAD chromatogram (λ = 325 nm) - chloroform extract from A. gmelinii Weber ex Stechm.
Title: Apigenin and hesperidin augment the toxic effect of doxorubicin against HepG2 cells Authors: Agnieszka Korga (agnieszka.korga@umlub.pl) Marta Ostrowska (martaostrowska@umlub.pl) Aleksandra Jozefczyk (ajozefczyk@pharmacognosy.org)... more
Title: Apigenin and hesperidin augment the toxic effect of doxorubicin against HepG2 cells Authors: Agnieszka Korga (agnieszka.korga@umlub.pl) Marta Ostrowska (martaostrowska@umlub.pl) Aleksandra Jozefczyk (ajozefczyk@pharmacognosy.org) Magdalena Iwan (magda.iwan@umlub.pl) Rafal Wojcik (rafal.wojcik@umlub.pl) Grazyna Zgorka (gzgorka@pharmacognosy.org) Mariola Herbet (mariolaherbet@umlub.pl) Gemma Vilarrubla (gemma5_5@hotmail.com) Jaroslaw Dudka (jaroslaw.dudka@umlub.pl) Version: 1 Date: 14 Mar 2019 Author’s response to reviews: Response to the Reviewers We would like to thank the Reviewers for their commitment, thoroughness and all remarks. We have not met such helpful comments for a long time which, we hope, helped us improve our work. In the following we address their concerns point by point.
A number of studies have confirmed anti-tumor activity of flavonoids and their ability to enhance the effectiveness of classical anticancer drugs. The mechanism of this phenomenon is difficult to explain because of the ambivalent nature... more
A number of studies have confirmed anti-tumor activity of flavonoids and their ability to enhance the effectiveness of classical anticancer drugs. The mechanism of this phenomenon is difficult to explain because of the ambivalent nature of these compounds. Many therapeutic properties of these compounds are attributed to their antioxidant activity; however, it is known that they can act as oxidants. The aim of this study was to assess the influence of apigenin and hesperidin on MCF-7 breast cancer cells with doxorubicin. The cytotoxic effect was determined using an MTT test and cell cycle analysis. To evaluate the possible interaction mechanism, reduced glutathione levels, as well as the DNA oxidative damage and the double strand breaks, were evaluated. Additionally, mRNA expression of genes related to DNA repair was assessed. It was demonstrated that flavonoids intensified the cytotoxic effect of doxorubicin despite flavonoids reduced oxidative damage caused by the drug. At the same...
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The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane... more
The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane fractions. Determination of antibacterial, antifungal and antioxidant activities of both extracts was also performed. RP-HPLC/PDA and HPLC/ESI-QTOF-MS showed that there were some differences between the composition of both extracts. The most characteristic components of TM were rosmarinic acid, protocatechuic acid, luteolin 7-O-glucoside, and apigenin 7-O-glucuronide, while protocatechuic acid, luteolin 7-O-glucoside, luteolin 7-O-glucuronide, and eriodictyol predominated in TS. The content of polyplenols was higher in TS than in TM. The GC-MS analysis of the volatile fraction of both examined extracts revealed the presence of thymol and carvacrol. Additionally, sesquiterpenoids, fatty acids, and their ethyl esters were found in TM, and fatty acid methyl...
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Centaurea L. jest jednym z liczniejszych (czwartym, co do wielkości) rodzajów rodziny Asteraceae, należącym do grupy roślin okrytonasiennych (kwiatowych). Morfologia i chemizm tego rodzaju, wciąż jeszcze nie są dobrze poznane. Obejmuje on... more
Centaurea L. jest jednym z liczniejszych (czwartym, co do wielkości) rodzajów rodziny Asteraceae, należącym do grupy roślin okrytonasiennych (kwiatowych). Morfologia i chemizm tego rodzaju, wciąż jeszcze nie są dobrze poznane. Obejmuje on najczęściej wieloletnie, ale również roczne i dwuletnie gatunki roślin trawiastych i krzewinek. Rośliny z rodzaju Centaurea L. występują głównie w obszarze Morza Śródziemnego i Zachodniej Azji. Rodzaj ten jest jednym z najbogatszych pod względem występowania gatunków endemicznych. Szczególnie dużą liczbę gatunków Centaurea L. spotkać możemy w Turcji, głównie w południowo-zachodniej, centralnej i wschodniej części kraju. Badania etnofarmakologiczne tego rodzaju wykazały, że duża liczba tych roślin jest w wielu krajach używana w medycynie ludowej do leczenia różnych dolegliwości. W niniejszej pracy przedstawiono przegląd olejków eterycznych pozyskiwanych z wybranych 50 gatunków roślin rodzaju Centaurea L., ze szczególnym uwzględnieniem ich składu i z...
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Background Extracts from medicinal plants with phytochemicals with known antimicrobial properties can be an effective adjunct in the complex treatment of infectious diseases. This study aimed to evaluate the antimicrobial activity of... more
Background Extracts from medicinal plants with phytochemicals with known antimicrobial properties can be an effective adjunct in the complex treatment of infectious diseases. This study aimed to evaluate the antimicrobial activity of wormwood extracts collected in Kazakhstan (Artemisia gmelinii Weber ex Stechm.), along with their phytochemical analysis. Methods The ethanolic and chloroform extracts were subjected to HPLC combined with quadrupole time-of-flight mass spectrometry method. For quantitative assessment of antimicrobial activity, minimal inhibitory concentration (MIC) of the tested extracts was determined by micro-dilution broth method for the panel of the reference microorganisms. Minimal bactericidal concentration (MBC) or minimal fungicidal concentration (MFC) were also determined. Results LC/MS analysis showed the presence of 13 compounds in the tested extracts, including flavonoids: apigenin, luteolin, rutin, two O-methylated flavonols (isorhamnetin, rhamnazine), coum...
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ABSTRACT The essential oil of Chrysanthemum maximum Ram. (Leucanthemum maximum [Ram.] DC) was isolated and constituents were analysed by GC/MS.