Vijay Kumar
ICGEB-India, Virology, Adjunct
- Virology, Hepatitis B, Cell Cycle, DNA replication, Cancer Cell Biology, Molecular virology, Herbal Anticancer, DNA methylation, Cell Cycle regulation, Epigenetics & Chromatin Biology, and 18 moreExosomes, Hepatocellular Carcinoma, Ubiquitin E3 Ligase, Exosomes and viruses, ribosomal RNA, Ribosomal proteins, Human hepatitis B virus, Exosome, Myc Oncogene, Ribosomal DNA, Ribosome biogenesis, Oncogenic viruses, Hepatocyte, RNA Polymerase I, MOLECULAR BIOLOGY OF HBV, Ribosomal Protein, Eucaryotic DNA Replication, and Cell Cycle Analysisedit
Background: In Traditional Tibetan medicine, Yukyung Karne has been used for the treatment of ovarian cancer. Though Yukyung Karne has been reported to be clinically effective, the molecular mechanism of its anti-metstatic action remains... more
Background: In Traditional Tibetan medicine, Yukyung Karne has been used for the treatment of ovarian cancer.
Though Yukyung Karne has been reported to be clinically effective, the molecular mechanism of its anti-metstatic
action remains elusive.
Methods: The cytotoxic property of Yukyung Karne was evaluated by crystal violet staining while its ability to induce
ceramide production was analyzed by sphingomyelinase assay. The anti-metastatic property was investigated using
adhesion, invasion, migration and colony formation assays. The effect of Yukyung Karne on the expression of extracellular
matrix components, and epithelial and mesenchymal markers were evaluated by confocal microscopy and
western blotting.
Results: Yukyung Karne exhibited a strong anti-metastatic property by significantly reducing the invasion,
migration and colony formation ability of ovarian cancer cells. Besides it inhibited the levels of biomarkers
involved in epithelial to mesenchymal transition such as down-regulation of vimentin and N-cadherin and
up-regulation of epithelial E-cadherin. Yukyung Karne also induced the neutral sphingomyelinase II (nSMNaseII)
enzyme activity that is known to hydrolyze sphingomyelins into pro-apoptotic intracellular molecule ceramide.
Conclusions: The study provides some compelling evidences supporting the anti-metastatic potential of Yukyung
Karne which strongly suggests its possible usage as a promising alternative medicine. Thus, Yukyung Karne may
be used as an anticancer and anti-metastatic agent along with other conventional anticancer therapeutics to
increase their efficacy.
Though Yukyung Karne has been reported to be clinically effective, the molecular mechanism of its anti-metstatic
action remains elusive.
Methods: The cytotoxic property of Yukyung Karne was evaluated by crystal violet staining while its ability to induce
ceramide production was analyzed by sphingomyelinase assay. The anti-metastatic property was investigated using
adhesion, invasion, migration and colony formation assays. The effect of Yukyung Karne on the expression of extracellular
matrix components, and epithelial and mesenchymal markers were evaluated by confocal microscopy and
western blotting.
Results: Yukyung Karne exhibited a strong anti-metastatic property by significantly reducing the invasion,
migration and colony formation ability of ovarian cancer cells. Besides it inhibited the levels of biomarkers
involved in epithelial to mesenchymal transition such as down-regulation of vimentin and N-cadherin and
up-regulation of epithelial E-cadherin. Yukyung Karne also induced the neutral sphingomyelinase II (nSMNaseII)
enzyme activity that is known to hydrolyze sphingomyelins into pro-apoptotic intracellular molecule ceramide.
Conclusions: The study provides some compelling evidences supporting the anti-metastatic potential of Yukyung
Karne which strongly suggests its possible usage as a promising alternative medicine. Thus, Yukyung Karne may
be used as an anticancer and anti-metastatic agent along with other conventional anticancer therapeutics to
increase their efficacy.
