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DNA_in_bacteria

  • Developed for: Céline
  • Team: Espeli
  • Date: January 2023
  • Software: Fiji

Images description

3D images taken with a x60 objective

2 channels:

  1. DAPI: DNA
  2. TL phase: bacteria

Plugin description

  • Detect bacteria on the average intensity Z-projection of channel 2 with Omnipose
  • Detect DNA on the average intensity Z-projection of channel 1 with Omnipose
  • In each bacterium, return distances between bacterium centroid and DNA centroid

Dependencies

  • 3DImageSuite Fiji plugin
  • CLIJ Fiji plugin
  • Omnipose conda environment + bact_phase_omnitorch_0 and bact_fluor_omnitorch_0 models

Version history

Version 1 released on January 5, 2023.

Version 2 released on July 4, 2023: can handle images with multiple series and times.

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