962 | P a g e 3 0 J u n e 2 0 1 5 w w w. g j a r. o r g ABSTRACT Serial dilution of soil sample a... more 962 | P a g e 3 0 J u n e 2 0 1 5 w w w. g j a r. o r g ABSTRACT Serial dilution of soil sample and subsequent plating on nutrient agar resulted in isolation of 15 different bacteria isolates. Haemolytic activity on blood agar plate, three isolates UEO1, UEO9, UEO15 produced a clear zone around the colonies causing lysis of the blood. In the drop-collapsing test all the three strains collapsed the oil drop thus producing a flat drop and also produced a clear zone around the oil indicating ability to displace oil at the range between 5.2-5.7cm when tested using oil displacement method. All these results confirmed the ability of the three strains to produce surface active molecules. But when these isolates where subjected to emulsifications assay there activity varied having an emulsification index of 78.90%, 58.80% and 56.63% for UEO15, UEO1 and UEO9 respectively. The isolates were identified as Serratia marcescens UEO15, Bacillus firmus UEO9 and Bacillus licheniformis UEO1; these isolates (Serratia marcescens UEO15, Bacillus firmus UEO9 and Bacillus licheniformis UEO1) were subjected to biosurfactant production and they produced different amount 12.5(1.34g/l), 5.78 (0.27g/l), and 9.16(0.6g/l) respectively for both crude and purified. Since the aim of the research was on Serratia marcescens, this isolate was concentrated upon and further characterized. The TLC has an Rf value of 0.75 and gave red colour spot when sprayed with ninhydrin classifying it as a lipopeptide; the GCMS revealed the presence of palmitic acid, oleic acid, 1-[[[(2-aminoethoxyhydroxyphosphonyl]oxy] which are characteristic features of phospholipids; the FTIR revealed important functional groups (phosphine,P-H 3 ; amine, N-H and carboxylic acid, C=O) that defined the surfactant to be a phospholipid. The effectiveness of the phospholipid of SMUEO15 in oil recovery was also tested and the result revealed that 78% and 59% of crude oil and kerosene was recovered as compared to 10% and 25% obtained by distilled water respectively. This result confirms that biosurfactant are active biomolecules that can be used in oil recovery and bioremediation of hydrocarbon polluted environment. 963 | P a g e 3 0 J u n e 2 0 1 5 w w w. g j a r. o r g
A bstract This study was designed to assess the potential of Arachis hypogaea (groundnut) to rest... more A bstract This study was designed to assess the potential of Arachis hypogaea (groundnut) to restore lead (Pb) contaminated soil. Pot experiment was conducted. Viable seeds were planted into five kilogram of the experimental soil placed in each plastic pot. Phytoremediation of soil contaminated with 0ppm (control), 5ppm, 10ppm, 15ppm, 20ppm and 25ppm heavy metal (Pb) were studied for a period of 12weeks under natural condition. The bacterial counts ranged from 32×10 6 cfu/g to 10×10 6 cfu/g in Pb polluted soil remediated with Arachis hypogaea (A. hypogaea) while the total fungi counts ranged from 25 × 10 2 cfu/g to 1 × 10 2 cfu/g. Microorganisms isolated from the rhizosphere were identified as (leaf, stem, seeds and roots) of A. hypogaea were analyzed for heavy metal (Pb) uptake after 12 weeks. The plants mopped up substantial concentrations of Pb in the above biomass of the plant in the seeds (1.73ppm), stem (1.26ppm) and leaves (2.30ppm) compared to concentrations in the roots (1.27ppm). The phytoextraction ability of the plant was assessed in terms of its metal bioconcentration factor (BCF) and translocation factor (TF). It was observed that more of this element was translocated to leaves of the plant. The results obtained suggest that the plant (Arachis hypogaea) has phytoextraction potential and could be used in reclaiming soil polluted with Pb.
Juice was produced from watermelon and stored at room (28±2°C) and refrigeration (8°C) temperatur... more Juice was produced from watermelon and stored at room (28±2°C) and refrigeration (8°C) temperatures and was analyzed for its microbiological and nutritional qualities. The total aerobic bacterial, coliform, mold and yeast counts increased with time.
A study was designed to assess the phytoextraction potential of Glycine max L. for lead (Pb). Pot... more A study was designed to assess the phytoextraction potential of Glycine max L. for lead (Pb). Pots experiment was conducted. Viable seeds were planted in 5 kg of soil placed in each plastic pot having 0 ppm (control), 5 ppm, 10 ppm, 15 ppm, 20 ppm and 25 ppm of Pb respectively. The study was carried out for a period of 12 weeks under natural conditions. Physicochemical properties of the soil were determined using standard methods. The results revealed that pH, phosphorous and moisture contents increased while nitrogen and organic carbon contents decreased in polluted soil remediated with Glycine max L. compared to the unpolluted soil. Leaf, stem, seeds and roots of the plant were analyzed for Pb uptake after 12 weeks. The plants mopped up substantial concentration of Pb in the above plant biomass of the seeds (4.2 mg/kg), stem (1.37 mg/kg) and leaves (3.37 mg/kg) compared to concentrations in the roots (1.53 mg/kg). The phytoextraction ability of the plant was assessed in terms of its bioconcentration factor (BCF) and translocation factor (TF). It was observed that the levels of Pb in the roots and shoots after 12 weeks showed that more bioavailable pool of Pb was translocated from the root to seeds, leaves and stem in that order. The results obtained suggest that the plant has phytoextraction ability and could be used in restoring soil polluted with Pb.
962 | P a g e 3 0 J u n e 2 0 1 5 w w w. g j a r. o r g ABSTRACT Serial dilution of soil sample a... more 962 | P a g e 3 0 J u n e 2 0 1 5 w w w. g j a r. o r g ABSTRACT Serial dilution of soil sample and subsequent plating on nutrient agar resulted in isolation of 15 different bacteria isolates. Haemolytic activity on blood agar plate, three isolates UEO1, UEO9, UEO15 produced a clear zone around the colonies causing lysis of the blood. In the drop-collapsing test all the three strains collapsed the oil drop thus producing a flat drop and also produced a clear zone around the oil indicating ability to displace oil at the range between 5.2-5.7cm when tested using oil displacement method. All these results confirmed the ability of the three strains to produce surface active molecules. But when these isolates where subjected to emulsifications assay there activity varied having an emulsification index of 78.90%, 58.80% and 56.63% for UEO15, UEO1 and UEO9 respectively. The isolates were identified as Serratia marcescens UEO15, Bacillus firmus UEO9 and Bacillus licheniformis UEO1; these isolates (Serratia marcescens UEO15, Bacillus firmus UEO9 and Bacillus licheniformis UEO1) were subjected to biosurfactant production and they produced different amount 12.5(1.34g/l), 5.78 (0.27g/l), and 9.16(0.6g/l) respectively for both crude and purified. Since the aim of the research was on Serratia marcescens, this isolate was concentrated upon and further characterized. The TLC has an Rf value of 0.75 and gave red colour spot when sprayed with ninhydrin classifying it as a lipopeptide; the GCMS revealed the presence of palmitic acid, oleic acid, 1-[[[(2-aminoethoxyhydroxyphosphonyl]oxy] which are characteristic features of phospholipids; the FTIR revealed important functional groups (phosphine,P-H 3 ; amine, N-H and carboxylic acid, C=O) that defined the surfactant to be a phospholipid. The effectiveness of the phospholipid of SMUEO15 in oil recovery was also tested and the result revealed that 78% and 59% of crude oil and kerosene was recovered as compared to 10% and 25% obtained by distilled water respectively. This result confirms that biosurfactant are active biomolecules that can be used in oil recovery and bioremediation of hydrocarbon polluted environment. 963 | P a g e 3 0 J u n e 2 0 1 5 w w w. g j a r. o r g
A bstract This study was designed to assess the potential of Arachis hypogaea (groundnut) to rest... more A bstract This study was designed to assess the potential of Arachis hypogaea (groundnut) to restore lead (Pb) contaminated soil. Pot experiment was conducted. Viable seeds were planted into five kilogram of the experimental soil placed in each plastic pot. Phytoremediation of soil contaminated with 0ppm (control), 5ppm, 10ppm, 15ppm, 20ppm and 25ppm heavy metal (Pb) were studied for a period of 12weeks under natural condition. The bacterial counts ranged from 32×10 6 cfu/g to 10×10 6 cfu/g in Pb polluted soil remediated with Arachis hypogaea (A. hypogaea) while the total fungi counts ranged from 25 × 10 2 cfu/g to 1 × 10 2 cfu/g. Microorganisms isolated from the rhizosphere were identified as (leaf, stem, seeds and roots) of A. hypogaea were analyzed for heavy metal (Pb) uptake after 12 weeks. The plants mopped up substantial concentrations of Pb in the above biomass of the plant in the seeds (1.73ppm), stem (1.26ppm) and leaves (2.30ppm) compared to concentrations in the roots (1.27ppm). The phytoextraction ability of the plant was assessed in terms of its metal bioconcentration factor (BCF) and translocation factor (TF). It was observed that more of this element was translocated to leaves of the plant. The results obtained suggest that the plant (Arachis hypogaea) has phytoextraction potential and could be used in reclaiming soil polluted with Pb.
Juice was produced from watermelon and stored at room (28±2°C) and refrigeration (8°C) temperatur... more Juice was produced from watermelon and stored at room (28±2°C) and refrigeration (8°C) temperatures and was analyzed for its microbiological and nutritional qualities. The total aerobic bacterial, coliform, mold and yeast counts increased with time.
A study was designed to assess the phytoextraction potential of Glycine max L. for lead (Pb). Pot... more A study was designed to assess the phytoextraction potential of Glycine max L. for lead (Pb). Pots experiment was conducted. Viable seeds were planted in 5 kg of soil placed in each plastic pot having 0 ppm (control), 5 ppm, 10 ppm, 15 ppm, 20 ppm and 25 ppm of Pb respectively. The study was carried out for a period of 12 weeks under natural conditions. Physicochemical properties of the soil were determined using standard methods. The results revealed that pH, phosphorous and moisture contents increased while nitrogen and organic carbon contents decreased in polluted soil remediated with Glycine max L. compared to the unpolluted soil. Leaf, stem, seeds and roots of the plant were analyzed for Pb uptake after 12 weeks. The plants mopped up substantial concentration of Pb in the above plant biomass of the seeds (4.2 mg/kg), stem (1.37 mg/kg) and leaves (3.37 mg/kg) compared to concentrations in the roots (1.53 mg/kg). The phytoextraction ability of the plant was assessed in terms of its bioconcentration factor (BCF) and translocation factor (TF). It was observed that the levels of Pb in the roots and shoots after 12 weeks showed that more bioavailable pool of Pb was translocated from the root to seeds, leaves and stem in that order. The results obtained suggest that the plant has phytoextraction ability and could be used in restoring soil polluted with Pb.
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