ABSTRACT The objective of this study was to introduce a class of collagen-fiber reinforced bio-co... more ABSTRACT The objective of this study was to introduce a class of collagen-fiber reinforced bio-composite laminates as biomimetic of soft tissues. These novel all-natural bio-composite laminates include long collagen fibers from soft coral embedded in an alginate hydrogel matrix. Controlling the fiber orientation and volume fraction enabled the fabrication of laminates with wide range of mechanical behaviors. Four material systems were investigated in the current study having different fiber orientations: longitudinal (0°), transverse (90°), cross-plied (0/90°) and angle-plied (±30°). The range of Fiber volume fractions (FVFs) for the laminated membranes is between 0.21 and 0.31. The laminates were subjected to uniaxial loading, yielding hyperelastic stress–strain behavior. A hyperelastic finite element (FE) model was constructed for the heterogeneous laminate, based on the fiber and matrix hyperelastic material behavior and their FVF, in order to predict the overall bio-composite mechanical behavior. The predictions of the FE model were verified from the tested laminated systems. The FE model consisted of beam elements representing the collagen fibers embedded in the solid matrix (alginate). Good predictions were demonstrated by the proposed FE model compared with the tested bio-composites for all orientations up to 10% strain. The overall hyperelastic stress–strain behavior was in a similar range to known native soft tissues. In addition, the model allowed for examining the mechanical behavior of laminates with other FVFs. The new bio-composite material can be used for future soft tissue mimicry and repair.
Journal of the Mechanical Behavior of Biomedical Materials, 2014
Uniaxial tension was applied to selectively digested single lamellar human cadaveric annulus fibr... more Uniaxial tension was applied to selectively digested single lamellar human cadaveric annulus fibrosus specimens to investigate the role of different biomolecules in annular biomechanics. Single layered and inter-lamellar annulus fibrosus samples were obtained from 10 isolated cadaveric lumbar intervertebral discs in one of four orientations: longitudinal, transverse, radial, and circumferential. Within each orientation the samples were subjected to a selective enzymatic digestion protocol with collagenase, elastase, chondroitinase ABC, or 1× Phosphate Buffered Saline. Uniaxial tensile tests were performed to failure at a strain rate of 0.005s(-1). Failure stress and strain, and elastic moduli were compared among the digested conditions. The collagenase- and elastase-treated groups had the most significant effect on the mechanical properties among the orientation groups, decreasing the failure stress for both interlaminar and intralaminar groups. Collagenase-treated groups showed an increase in the failure strain following enzymatic digestion for the intralaminar groups and one interlaminar testing direction (circumferential). The chondroitinase ABC-treated group only had a significant impact on the single layer orientations, decreasing the failure stress and strain (intralaminar group). The digested properties described provide insights into the laminar mechanical behavior and the role of the molecular components to the annular mechanical behavior. Understanding annular mechanics may prove insightful in diagnosis, prevention and repair of debilitating intervertebral disc disorders and manufacturing of tissue-engineered annulus.
Nucleus pulposus replacement and augmentation has been proposed to restore disk mechanics in earl... more Nucleus pulposus replacement and augmentation has been proposed to restore disk mechanics in early stages of degeneration with the option of providing a minimally invasive procedure for pain relief to patients with an earlier stage of degeneration. The goal of this paper is to examine compressive stability of the intervertebral disk after either partial nucleus replacement or nuclear augmentation in the absence of denucleation. Thirteen human cadaver lumbar anterior column units were used to study the effects of denucleation and augmentation on the compressive mechanical behavior of the human intervertebral disk. Testing was performed in axial compression after incremental steps of partial denucleation and subsequent implantation of a synthetic hydrogel nucleus replacement. In a separate set of experiments, the disks were not denucleated but augmented with the same synthetic hydrogel nucleus replacement. Neutral zone, range of motion, and stiffness were measured. The results showed that compressive stabilization of the disk can be re-established with nucleus replacement even for partial denucleation. Augmentation of the disk resulted in an increase in disk height and intradiskal pressure that were linearly related to the volume of polymer implanted. Intervertebral disk instability, evidenced by increased neutral zone and ranges of motion, associated with degeneration can be restored by volume filling of the nucleus pulposus using the hydrogel device presented here.
ABSTRACT The objective of this study was to introduce a class of collagen-fiber reinforced bio-co... more ABSTRACT The objective of this study was to introduce a class of collagen-fiber reinforced bio-composite laminates as biomimetic of soft tissues. These novel all-natural bio-composite laminates include long collagen fibers from soft coral embedded in an alginate hydrogel matrix. Controlling the fiber orientation and volume fraction enabled the fabrication of laminates with wide range of mechanical behaviors. Four material systems were investigated in the current study having different fiber orientations: longitudinal (0°), transverse (90°), cross-plied (0/90°) and angle-plied (±30°). The range of Fiber volume fractions (FVFs) for the laminated membranes is between 0.21 and 0.31. The laminates were subjected to uniaxial loading, yielding hyperelastic stress–strain behavior. A hyperelastic finite element (FE) model was constructed for the heterogeneous laminate, based on the fiber and matrix hyperelastic material behavior and their FVF, in order to predict the overall bio-composite mechanical behavior. The predictions of the FE model were verified from the tested laminated systems. The FE model consisted of beam elements representing the collagen fibers embedded in the solid matrix (alginate). Good predictions were demonstrated by the proposed FE model compared with the tested bio-composites for all orientations up to 10% strain. The overall hyperelastic stress–strain behavior was in a similar range to known native soft tissues. In addition, the model allowed for examining the mechanical behavior of laminates with other FVFs. The new bio-composite material can be used for future soft tissue mimicry and repair.
Journal of the Mechanical Behavior of Biomedical Materials, 2014
Uniaxial tension was applied to selectively digested single lamellar human cadaveric annulus fibr... more Uniaxial tension was applied to selectively digested single lamellar human cadaveric annulus fibrosus specimens to investigate the role of different biomolecules in annular biomechanics. Single layered and inter-lamellar annulus fibrosus samples were obtained from 10 isolated cadaveric lumbar intervertebral discs in one of four orientations: longitudinal, transverse, radial, and circumferential. Within each orientation the samples were subjected to a selective enzymatic digestion protocol with collagenase, elastase, chondroitinase ABC, or 1× Phosphate Buffered Saline. Uniaxial tensile tests were performed to failure at a strain rate of 0.005s(-1). Failure stress and strain, and elastic moduli were compared among the digested conditions. The collagenase- and elastase-treated groups had the most significant effect on the mechanical properties among the orientation groups, decreasing the failure stress for both interlaminar and intralaminar groups. Collagenase-treated groups showed an increase in the failure strain following enzymatic digestion for the intralaminar groups and one interlaminar testing direction (circumferential). The chondroitinase ABC-treated group only had a significant impact on the single layer orientations, decreasing the failure stress and strain (intralaminar group). The digested properties described provide insights into the laminar mechanical behavior and the role of the molecular components to the annular mechanical behavior. Understanding annular mechanics may prove insightful in diagnosis, prevention and repair of debilitating intervertebral disc disorders and manufacturing of tissue-engineered annulus.
Nucleus pulposus replacement and augmentation has been proposed to restore disk mechanics in earl... more Nucleus pulposus replacement and augmentation has been proposed to restore disk mechanics in early stages of degeneration with the option of providing a minimally invasive procedure for pain relief to patients with an earlier stage of degeneration. The goal of this paper is to examine compressive stability of the intervertebral disk after either partial nucleus replacement or nuclear augmentation in the absence of denucleation. Thirteen human cadaver lumbar anterior column units were used to study the effects of denucleation and augmentation on the compressive mechanical behavior of the human intervertebral disk. Testing was performed in axial compression after incremental steps of partial denucleation and subsequent implantation of a synthetic hydrogel nucleus replacement. In a separate set of experiments, the disks were not denucleated but augmented with the same synthetic hydrogel nucleus replacement. Neutral zone, range of motion, and stiffness were measured. The results showed that compressive stabilization of the disk can be re-established with nucleus replacement even for partial denucleation. Augmentation of the disk resulted in an increase in disk height and intradiskal pressure that were linearly related to the volume of polymer implanted. Intervertebral disk instability, evidenced by increased neutral zone and ranges of motion, associated with degeneration can be restored by volume filling of the nucleus pulposus using the hydrogel device presented here.
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