John Iskra

ABSTRACTHigh-parameter tissue imaging enables detailed molecular analysis of single cells in their spatial environment. However, the comprehensive characterization and mapping of tissue states through multimodal imaging across different physiological and pathological conditions requires data integration across multiple imaging systems. Here, we introduce MIAAIM (Multi-omics Image Alignment and Analysis by Information Manifolds) a modular, reproducible computational framework for aligning data across bioimaging technologies, modeling continuities in tissue states, and translating multimodal measures across tissue types. We demonstrate MIAAIM’s workflows across diverse imaging platforms, including histological stains, imaging mass cytometry, and mass spectrometry imaging, to link cellular phenotypic states with molecular microenvironments in clinical biopsies from multiple tissue types with high cellular complexity. MIAAIM provides a robust foundation for the development of computatio...

In this paper we show that the category of frames, and, thus, the cate- gory of locales is 'rigid'. This means that every endo-equivalence on them is isomorphic to the identity functor. To reach this result we prove new results concerning the number of automorphisms between frames and new results concerning the order preserving properties of endo-equivalences.

UMI, ProQuest ® Dissertations & Theses. The world's most comprehensive collection of dissertations and theses. Learn more... ProQuest, Decidability in algebraic geometry. by Iskra, John James, Ph.D., THE UNIVERSITY OF TENNESSEE, 2004, 40 pages; 3141809. Abstract: ...