Abstract
A piezoelectric immunosensor was developed for detecting Mycobacteria Tuberculosis (M. TB). Protein A was modified onto a silver-coated piezoelectric quartz-crystal sensor. Antibodies could be bond onto the crystal surface in an ordered orientation through protein A. An impendence analyzer was employed to record the admittance-frequency curve. The experimental results showed a successful and less-rigid bonding of protein A and antibodies. Compared with other traditional detection methods of TB, the method suggested in this paper was sensitive, selective and effective. The nonspecific response was limited by using control antibody-rabbit anti-honey bee venom (anti-HBV). A glycine-HCl buffer solution (pH = 2.4) was used to release antibodies from a crystal coated with protein A, and 0.5 mg/mL anti-TB was used to remove TB from a crystal that was bonded by an antibody. Good reusability was exhibited. Spike samples of sputum and saliva from normal people with and without adding M. tuberculosis were diagnosed using the proposed method. Good results were obtained.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. Kochi, Immunobiology, 1994, 191, 325.
C. J. Murray, K. Styblo, and A. Rouillon, Bull. Int. Union Tuberc., 1990, 65, 6.
P. Nunn and M. Felten, Tubercle Lung Dis., 1994, 75, 163.
B. P. Vareldzis, J. Grosset, I. De Kantor, J. Crafton, A. Laszlo, M. Feltyen, M. C. Raviglion, and A. Kochi, Tubercle Lung Dis., 1994, 75, 1.
L. Arango, A. W. Brewin, and J. F. T. Murray, Am. Rev. Respir. Dis., 1978, 108, 805.
M. A. Khan, D. M. Kovat, B. Bachus, M. E. Whitcomb, J. S. Bordy, and G. L. Snider, Am. J. Med., 1977, 62, 31.
J. C. Weissler, Am. J. Med. Sci., 1993, 305, 52.
G. S. Favez, S. Jequier, and P. Vulliemoz, Am. Rev. Respir. Dis., 1966, 94, 896.
S. O. Freedman, J. Dolovich, R. Tucotte, and F. Gault, Am. Rev. Respir. Dis., 1966, 94, 896.
R. C. Parlett, G. P. Youmans, C. Rehr, and W. Lester, Am. Rev. Tuberc., 1958, 77, 462.
Y. Takahashi, Am. Rev. Respir. Dis., 1962, 85, 708.
A. C. Nicholls, J. Clin. Pathol., 1975, 28, 850.
E. Engvall and P. Perlman, J. Immunol., 1972, 109, 29.
R. Dhand, N. K. Gunguli, C. Vanishnavi, R. Gilhatra, and S. K. Malik, Med. Microbiol., 1988, 26, 241.
C. H. Wu, M. C. Funn, and I. J. Lun, Tubercle, 1989, 70, 37.
V. V. Radharkrishman and A. Mathai, India J. Med. Res., 1990, 91, 355.
T. M. Daniel, Rev. Infect. Dis., 1989, 11(supplement), S471.
G. D. Georgiev and A. C. McDougall, India J. Lepr., 1987, 59, 373.
G. Z. Sauerbrey, Z. Phys., 1959, 155, 206.
C. Raman Suri, Manoj Raje, and Gyan C. Mishra, Biosens. & Bioelectron., 1994, 9, 325.
G. G. Guilbault, B. Hock, and R. Schmid, Biosens. & Bioelectron., 1992, 7, 411.
M. Plomer, G. G. Guilbault, and B. Hock, Enzyme Microb. Technol., 1992, 14, 230.
J. J. Langone, J. Immunological Methods, 1982, 55, 277.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
He, F., Zhang, L. Rapid Diagnosis of M. tuberculosis Using a Piezoelectric Immunosensor. ANAL. SCI. 18, 397–401 (2002). https://doi.org/10.2116/analsci.18.397
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.18.397