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Oligomerization of the potato virus X 25-kD movement protein

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Abstract

A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg2+-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein.

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Abbreviations

AD, BD:

activator and DNA-binding domains of the yeast Ga14 protein, respectively

GA:

glutaraldehyde

PVX:

potato virus X

TGB(1–3) proteins:

proteins encoded by the genes (1–3) of the triple gene block

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Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    A. D. Leshchiner, D. V. Rakitina, A. G. Solovyev, S. Yu. Morozov & N. O. Kalinina

  2. Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, ul. Timiryazevskaya 42, 127550, Moscow, Russia

    A. D. Leshchiner, V. K. Vishnichenko & A. G. Solovyev

  3. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    E. A. Minina

Authors
  1. A. D. Leshchiner
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  2. E. A. Minina
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  3. D. V. Rakitina
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  4. V. K. Vishnichenko
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  5. A. G. Solovyev
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  6. S. Yu. Morozov
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  7. N. O. Kalinina
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Corresponding author

Correspondence to N. O. Kalinina.

Additional information

Original Russian Text © A. D. Leshchiner, E. A. Minina, D. V. Rakitina, V. K. Vishnichenko, A. G. Solovyev, S. Yu. Morozov, N. O. Kalinina, 2008, published in Biokhimiya, 2008, Vol. 73, No. 1, pp. 62–68.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM07-197, October 21, 2007.

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Leshchiner, A.D., Minina, E.A., Rakitina, D.V. et al. Oligomerization of the potato virus X 25-kD movement protein. Biochemistry Moscow 73, 50–55 (2008). https://doi.org/10.1134/S0006297908010070

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