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Ultrasound-Assisted Aqueous Enzymatic Extraction of Oil from Pomegranate Seeds

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Abstract

Pomegranate seed oil presents nutraceutical properties. Industrial processes for extraction of edible oils from seeds generally involve a solvent extraction step. Safety considerations on the use of organic solvents prompted attempts to develop aqueous extraction. However, aqueous extraction processes are usually characterized by low oil yields. The objective of this work was to overcome these low yields by using enzymes. The pomegranate seeds were treated with two enzymes—cellulase and Peclyve V. The extraction temperature, the time, the enzyme concentration, and the water/seeds ratio were varied between 35 and 55 °C, 2 and 8 h, 2 and 4 % w/w, and 2/1 and 6/1 mL/g, respectively. The optimum yield accomplished (15.33 g oil/100 g dry seeds at 2 h or 81 % oil recovery) was comparable to the yields obtained by other extraction methods, such as normal stirring, cold pressing, superheated fluid extraction, indirect ultrasound-assisted extraction and supercritical extraction, (4.29–25.11 g oil/100 g seeds) at similar or longer extraction times (10 min–72 h). In addition, this work studies the enhancement of aqueous enzymatic extraction of pomegranate seed oil by ultrasound probe. It was found that only 10 min in water are needed to recover oil with a yield of 18.15 g oil/100 g dry seeds, a value similar to or higher than those of the conventional procedures. Thus, the use of ultrasounds increased the yield of enzymatic extraction by 18.4 % and reduced the extraction time by 91.7 %.

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References

  1. Türkyılmaz, M., Tagi, S., Dereli, U., Özkan, M.: Effects of various pressing programs and yields on the antioxidant activity, antimicrobial activity, phenolic content and colour of pomegranate juices. Food Chem. 138, 1810–1818 (2013)

    Article  Google Scholar 

  2. Mohagheghi, M., Rezaei, K., Labbafi, M., Mousavi, S.M.E.: Pomegranate seed oil as a functional ingredient in beverages. Eur. J. Lipid Sci. Tech. 113, 730–736 (2011)

    Article  Google Scholar 

  3. Fernandes, L., Pereira, J.A., Lopéz-Cortés, I., Salazar, D.M., Ramalhosa, E., Casal, S.: Fatty acid, vitamin E and sterols composition of seed oils from nine different pomegranate (Punica granatum L.) cultivars grown in Spain. J. Food Compos. Anal. 39, 13–22 (2015)

    Article  Google Scholar 

  4. Eikani, M.H., Golmohammad, F., Homami, S.S.: Extraction of pomegranate (Punica granatum L.) seed oil using superheated hexane. Food Bioprod. Process. 90, 32–36 (2012)

    Article  Google Scholar 

  5. Al-Maiman, S.A., Ahmad, D.: Changes in physical and chemical properties during pomegranate (Punica granatum L.) fruit maturation. Food Chem. 76, 437–441 (2002)

    Article  Google Scholar 

  6. Lansky, E.P., Newman, R.A.: Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J. Ethnopharmacol 109, 177–206 (2007)

    Article  Google Scholar 

  7. Abbasi, H., Rezaei, K., Rashidi, L.: Extraction of essential oils form the seeds of pomegranate using organic solvents and supercritical CO2. J. Am. Oil Chem. Soc. 85, 83–89 (2008)

    Article  Google Scholar 

  8. Tong, P., Kasuga, Y., Khoo, C.S.: Liquid chromatographic mass spectrometric method for detection of estrogen in commercial oils and in fruit seed oils. J. Food Compos. Anal. 19, 150–156 (2006)

    Article  Google Scholar 

  9. Yamasaki, M., Kitagawa, T., Koyanagi, N., Chujo, H., Maeda, H., KohnoMurase, J., Imamura, J., Tachibana, H., Yamada, K.: Dietary effect of pomegranate seed oil on immune function and lipid metabolism in mice. Nutrition 22, 54–59 (2006)

    Article  Google Scholar 

  10. Meerts, I.A.T.M., Verspeek-Rip, C.M., Buskens, C.A.F., Keizer, H.G., Bassaganya-Riera, J., Jouni, Z.E., van Huygevoort, A.H.B.M., van Otterdijk, F.M., van de Wart, E.J.: Toxicological evaluation of pomegranate seed oil. Food Chem. Toxicol. 47, 1085–1092 (2009)

    Article  Google Scholar 

  11. Asadpour, E., Boroushaki, M.T., Sadeghnia, H.: Protective effect of pomegranate seed oil against gentamicin induced nephrotoxicity in rat. Toxicol. Lett. 196, 232 (2010)

    Article  Google Scholar 

  12. Melo, I.L.P., Carvalho, E.B.T., Silva, A.M.O., ManciniFilho, J.: Effects of pomegranate seed oil on lipoperoxidation and activity of antioxidant enzymes in liver and brain of rats. Free Radic. Biol. Med. 49, 189 (2010)

    Article  Google Scholar 

  13. Park, H.M., Moon, E., Kim, A.J., Kim, M.H., Lee, S., Lee, J.B., Park, Y.K., Jung, H., Kim, Y.B., Kim, S.Y.: Extract of Punica granatum inhibits skin photoaging induced by UVB irradiation. Int. J. Dermatol. 49, 276–282 (2010)

    Article  Google Scholar 

  14. Qu, W., Pan, Z., Ma, H.: Extraction modeling and activities of antioxidants from pomegranate marc. J. Food Eng. 99, 16–23 (2010)

    Article  Google Scholar 

  15. Kalamara, E., Goula, A.M., Adamopoulos, K.G.: An integrated process for utilization of pomegranate wastes-Seeds. Innov. Food Sci. Emerg Technol. 27, 144–153 (2015)

    Article  Google Scholar 

  16. Tian, Y., Xu, Z., Zheng, B., Lo, Y.M.: Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil. Ultrason. Sonochem. 20, 202–208 (2013)

    Article  Google Scholar 

  17. Goula, A.M., Adamopoulos, K.G.: A method for pomegranate seed application in food industries: seed oil encapsulation. Food Bioprod. Process. 90, 639–652 (2012)

    Article  Google Scholar 

  18. Abbasi, H., Rezaei, K., Emamdjomeh, Z., Mousavi, S.M.E.: Effect of various extraction conditions on the phenolic contents of pomegranate seed oil. Eur. J. Lipid Sci. Technol. 110, 435–440 (2008)

    Article  Google Scholar 

  19. Liu, G., Xu, X., Hao, Q., Gao, Y.: Supercritical CO2 extraction optimization of pomegranate (Punica granatum L.) seed oil using response surface methodology. Lebensm. Wiss. Technol. 42, 1491–1495 (2009)

    Article  Google Scholar 

  20. Rosenthal, A., Pyle, D.L., Niranja, K.: Aqueous and enzymatic processes for edible oil extraction. Enz. Microbial. Technol. 19, 402–420 (1996)

    Article  Google Scholar 

  21. Sharma, A., Khare, S.K., Gupta, M.N.: Enzyme-assisted aqueous extraction of oil from peanut seeds. J. Am. Oil Chem. Soc. 78, 949–951 (2001)

    Article  Google Scholar 

  22. Rosenthal, A., Pyle, D.L., Niranjan, K., Gilmour, S., Trinca, L.: Combined effect of operational variables and enzyme activity on aqueous enzymatic extraction of oil and protein from soybean. Enz. Microbial Technol 28, 499–509 (2001)

    Article  Google Scholar 

  23. Towa, L.T., Kapchie, V.N., Hauck, C., Murphy, P.A.: Enzyme-assisted aqueous extraction of oil from isolated oleosomes of soybean flour. J. Am. Oil Chem. Soc. 87, 347–354 (2010)

    Article  Google Scholar 

  24. Shah, S., Sharma, A., Gupta, M.N.: Extraction of oil from Jatropha curcas L. seed kernels by combination of ultrasonication and aqueous enzymatic oil extraction. Bioresour. Technol. 96, 121–123 (2005)

    Article  Google Scholar 

  25. Latif, S., Diosady, L.L., Anwar, F.: Enzyme-assisted aqueous extraction of oil and protein from canola (Brassica napus L.) seeds. Eur. J. Lipid Sci. Technol. 110, 887–892 (2008)

    Article  Google Scholar 

  26. Passos, C.P., Yilmaz, S., Silva, C.M., Coimbra, M.A.: Enhancement of grape seed oil extraction using a cell wall degrading enzyme cocktail. Food Chem. 115, 48–53 (2009)

    Article  Google Scholar 

  27. Latif, S., Anwar, F.: Effect of aqueous enzymatic processes on sunflower oil quality. J. Am. Oil Chem. Soc. 86, 393–400 (2009)

    Article  Google Scholar 

  28. Nyam, K.L., Tan, C.P., Lai, O.M., Long, K., Man, Y.B.C.: Enzyme-assisted aqueous extraction of kalahari melon seed oil: optimization using response surface methodology. J. Am. Oil Chem. Soc. 86, 1235–1240 (2009)

    Article  Google Scholar 

  29. Jiang, L., Hua, D., Wang, Z., Xu, S.: Aqueous enzymatic extraction of peanut oil and protein hydrolysates. Food Bioprod. Process. 88, 233–238 (2010)

    Article  Google Scholar 

  30. Latif, S., Diosady, L.L., Anwar, F.: Aqueous enzymatic sesame oil and protein extraction. Food Chem. 125, 679–684 (2011)

    Article  Google Scholar 

  31. Zhang, Y., Li, S., Yin, C., Jiang, D., Yan, F., Xu, T.: Response surface optimisation of aqueous enzymatic oil extraction from bayberry (Myrica rubra) kernels. Food Chem. 135, 304–308 (2012)

    Article  Google Scholar 

  32. Qi, Q.: Study on extraction of pumpkin seed oil by aqueous enzymatic method. J. Anhui Agric. Sci. 40, 7410–7413 (2012)

    Google Scholar 

  33. Jiao, J., Li, Z.G., Gai, Q.Y., Li, X.J., Wei, F.Y., Fu, Y.J., Ma, W.: Microwave-assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical propertiesm fatty acid compositions and antioxidant activities. Food Chem. 147, 17–24 (2014)

    Article  Google Scholar 

  34. Kalia, V.C., Sadhana, Rashmi Lal, Gupta, M.N.: Using enzymes for oil recovery from edible seeds. J. Sci. Ind. Res. 60, 298–310 (2001)

    Google Scholar 

  35. Vilkhu, K., Mawson, R., Simins, L., Bates, D.: Applications and opportunities for ultrasound assisted extraction in the food industry—a review. Innov. Food Sci. Emerg. Technol. 9, 161–169 (2008)

    Article  Google Scholar 

  36. Wu, H., Zhu, J., Diao, W., Wang, C.: Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata). Carbohyd. Polym. 113(26), 314–324 (2014)

    Article  Google Scholar 

  37. Liu, Y., Gong, G., Zhang, J., Jia, S., Li, F., Wang, Y., Wu, S.: Response surface optimization of ultrasound-assisted enzymatic extraction polysaccharides from Lycium barbarum. Carbohyd. Polym. 110(22), 278–284 (2014)

    Article  Google Scholar 

  38. Capelo, J.L., Ximénez-Embún, P., Madrid-Albarrán, Y., Cámara, C.: Enzymatic probe sonication: enhancement of protease-catalyzed hydrolysis of selenium bound to proteins in yeast. Anal. Chem. 76, 233–237 (2004)

    Article  Google Scholar 

  39. Cabanero, A.I., Madrid, Y., Camara, C.: Enzymatic probe sonication extraction of Se in animal-based food samples: a new perspective on sample preparation for total and Se speciation analysis. Anal. Bioanal. Chem. 381, 373–379 (2005)

    Article  Google Scholar 

  40. Siwek, M., Noubar, A.B., Bergmann, J., Niemeyer, B., Galunsky, B.: Enhancement of enzymatic digestion of Antarctic krill and successive extraction of selenium organic compounds by ultrasound treatment. Anal. Bioanal. Chem. 384, 244–249 (2006)

    Article  Google Scholar 

  41. Kaderides, K., Goula, A.M., Adamopoulos, K.G.: A process for turning pomegranate peels into a valuable food ingredient using ultrasound-assisted extraction and encapsulation. Innov. Food Sci. Emerg. Technol. 31, 204–215 (2015)

    Article  Google Scholar 

  42. Cochran, W.G., Cox, G.M.: Some methods for the study of response surfaces. Experimental designs, pp. 335–375. Wiley, New York (1992)

    Google Scholar 

  43. Zhang, Z.S., Wang, L.J., Li, D., Jiao, S.S., Chen, X.D., Mao, Z.H.: Ultrasound-assisted extraction of oil from flaxseed. Sep. Purif. Technol. 62, 192–198 (2008)

    Article  Google Scholar 

  44. Yusoff, M.M., Gordon, M.H., Niranjan, K.: Aqueous enzyme assisted oil extraction from oilseeds and emulsion de-emulsifying methods: a review. Trends Food Sci. Technol. 41, 60–82 (2015)

    Article  Google Scholar 

  45. Zuñiga, M.E., Soto, C., Mora, A., Chamy, R., Lema, J.M.: Enzymic pre-treatment of Guevina avellana mol oil extraction by pressing. Process. Biochem. 39, 51–57 (2003)

    Article  Google Scholar 

  46. Gros, C., Lanoiselle, J.L., Vorobiev, E.: Towards an alternative extraction process for linseed oil. Chem. Eng. Res. Des. 81, 1059–1065 (2003)

    Article  Google Scholar 

  47. Koponen, J.M., Happonen, A.M., Auriola, S., Kontkanen, H., Buchert, J., Poutanen, K.S., Torronen, A.R.: Characterization and fate of black currant and bilberry flavonols in enzyme-aided processing. J. Agric. Food Chem. 56, 3136–3144 (2008)

    Article  Google Scholar 

  48. Hanmoungjai, D.L., Pyle, D.E., Niranjan, K.: Enzymatic process for extraction oil and protein from rice bran. J. Am. Oil Chem. Soc. 78, 817–821 (2001)

    Article  Google Scholar 

  49. Zheng, H., Hwang, I., Kim, S., Lee, S., Chung, S.: Optimization of carbohydrate-hydrolyzing enzyme aided polyphenol extraction from unripe apples. J. Korean Soc. Appl. Biol. Chem. 53, 342–350 (2010)

    Article  Google Scholar 

  50. Landbo, A.K., Meyer, A.S.: Enzyme-assisted extraction of antioxidative phenols from black current juice press residues (Ribes nigrum). J. Agric. Food Chem. 49, 3169–3177 (2001)

    Article  Google Scholar 

  51. Costoya, N., Sineiro, J., Pinelo, M., Rubilar, M., Nuñez, M.J.: Enzyme-aided extraction of polyphenols from grape pomace. Electron. J. Environ. Agric. Food Chem. 9, 696–705 (2010)

    Google Scholar 

  52. Yin, X.L., You, Q.H., Jiang, Z.H.: Optimization of enzyme assisted extraction of polysaccharides from Tricholoma matsutake by response surface methodology. Carbohydr. Polym. 86, 1358–1364 (2011)

    Article  Google Scholar 

  53. Fu, Y.-J., Liu, W., Zu, Y.-G., Tong, M.-H., Li, S.-M., Yan, M.-M., Efferth, T., Luo, H.: Enzyme assisted extraction of luteolin and apigenin from pigeonpea leaves. Food Chem. 111, 508–512 (2008)

    Article  Google Scholar 

  54. Santala, O., Lehtinen, P., Nordlund, E., Suortti, T., Poutanen, K.: Impact of water content on the solubilisation of arabinoxylan during xylanase treatment of wheat bran. J. Cerealic. Sci. 54, 187–194 (2011)

    Article  Google Scholar 

  55. Hammed, A.M., Jaswir, I., Amid, A., Alam, Z., Asiyanbi-H, T.T., Ramli, N.: Enzymatic hydrolysis of plants and algae for extraction of bioactive compounds. Food Rev. Int. 29, 352–370 (2013)

    Article  Google Scholar 

  56. Heo, S.J., Lee, K.W., Song, C.B., Jeon, Y.J.: Antioxidant activity of enzymatic extracts from brown seaweeds. Algae 18, 71–81 (2003)

    Article  Google Scholar 

  57. Siriwardhana, N., Jeon, Y.-J., Kim, S.-H., Ha, J.-H., Heo, S.-J., Lee, K.-W.: Enzymatic hydrolysis for effective extraction of antioxidative compounds from Hizikia fusiformis. Algae 19, 59–68 (2004)

    Article  Google Scholar 

  58. Sineiro, J., Domínguez, H., Núñez, M.J., Lema, J.M.: Optimization of the enzymatic treatment during aqueous oil extraction from sunflower seeds. Food Chem. 61, 467–474 (1998)

    Article  Google Scholar 

  59. Zhang, S.B., Wang, Z., Xu, S.Y.: Optimization of the aqueous enzymatic extraction of rapeseed oil and protein hydrolysates. J. Am. Oil Chem. Soc. 84, 97–105 (2007)

    Article  Google Scholar 

  60. Teixeira, C.B., Macedo, G.A., Macedo, J.A., da Silva, L.H.M., Rodrigues, A.M.D.C.: Simultaneous extraction of oil and antioxidant compounds from oil palm fruit (Elaeis guineensis) by an aqueous enzymatic process. Bioresour. Technol. 129, 575–581 (2013)

    Article  Google Scholar 

  61. Bocevska, M., Karlović, D., Turkulov, J., Pericin, D.: Quality of corn germ oil obtained by aqueous enzymatic extraction. J. Am. Oil Chem. Soc. 70, 1273–1277 (1993)

    Article  Google Scholar 

  62. Balvardi, M., Rezaei, K., Mendiola, J.A., Ibáñez, E.: Optimization of the aqueous enzymatic extraction of oil from Iranian wild almond. J. Am. Oil Chem. Soc. 92, 985–992 (2015)

    Article  Google Scholar 

  63. Kalia, V.C., Rashmi, S.L.: Using enzymes for oil recovery from edible seeds. J. Sci. Ind. Res. 60, 298–310 (2001)

    Google Scholar 

  64. Goula, A.M.: Ultrasound-assisted extraction of pomegranate seed oil–Kinetic modeling. J. Food Eng. 117, 492–498 (2013)

    Article  Google Scholar 

  65. Dong, J., Liu, Y., Liang, Z., Wang, W.: Investigation on ultrasound-assisted extraction of salvianolic acid B from Salvia miltiorrhiza root. Ultrason. Sonochem. 17, 61–65 (2010)

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Technology, School of Agriculture, Forestry and Natural Environment, Aristotle University, 541 24, Thessaloniki, Greece

    Athanasia M. Goula, Anna Papatheodorou, Sofia Karasavva & Kyriakos Kaderides

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  1. Athanasia M. Goula
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  2. Anna Papatheodorou
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  3. Sofia Karasavva
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Goula, A.M., Papatheodorou, A., Karasavva, S. et al. Ultrasound-Assisted Aqueous Enzymatic Extraction of Oil from Pomegranate Seeds. Waste Biomass Valor 9, 1–11 (2018). https://doi.org/10.1007/s12649-016-9740-9

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