Abstract
Total antioxidant activity was evaluated in 40 tuber-bearing Solanum species. Accessions identified in a broad screen as having high antioxidant activity were fine screened via high performance liquid chromatography (HPLC) to determine specific phenolic compounds present. The objective of the study was to identify tuber-bearing Solanum species that are higher in antioxidants than in currently available cultivated varieties and breeding lines, and to investigate the variability for this trait among accessions within species and genotypes within accessions. The identified accessions will be used as a source of germplasm to develop potato cultivars containing increased levels of antioxidant compounds. In a broad screen for total antioxidant activity, the 40 tuber-bearing species showed a wider range of variability than cultivated varieties and breeding lines. Solanum pinnatisectum and S. jamesii accessions consistently ranked among the highest in antioxidant activity and phenolic content. Based on the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, antioxidant activity in the wild species ranged from 48 to 824 μg trolox eq/gfw. HPLC analysis revealed that the phenolic content of these species was primarily composed of chlorogenic and caffeic acids. Other phenolics identified were p-coumaric acid, rutin hydrate, vanillic acid, epicatechin, t-cinnamic acid, gallic acid, and salicylic acid. The highest phenolic content found among the accessions was five-fold higher than the highest of the cultivated genotypes.
Resumen
La actividad antioxidante total fue evaluada en 40 especies tuberíferas de Solanum. Las accesiones que mostraron actividad antioxidante en un tamizado amplio fueron sometidas a un tamizado más fino por medio de cromatografía líquida (HPCL) para determinar los compuestos fenólicos específicos presentes. El objetivo de este estudio fue identificar las especies tuberíferas de Solanum que tienen contenido más alto de antioxidantes que las variedades y líneas de mejoramiento e investigar la variabilidad de esta característica entre las accesiones dentro de especies y genotipos. Las accesiones identificadas serán usadas como fuente de germoplasma para desarrollar cultivares de papa que contengan niveles altos de compuestos antioxidantes. En un tamizado amplio para actividad total antioxidante, las 40 especies tuberíferas mostraron un rango más amplio de variabilidad que las variedades cultivadas y que las líneas mejoradas. Las accesiones de Solanum pinnatisectum y S. jamesii dieron consistentemente grados más altos en actividad antioxidante y contenido fenólico. En base a la prueba 2, 2-difenil-1-picrilhidrazil (DPPH), la actividad antioxidante en las especies silvestres fue de 48 a 824 μg trolox eq/gfw. El análisis HPLC reveló que el contenido de estas especies estaba principalmente compuesto de los ácidos clorogénico y cafeico. Otros fenoles identificados fueron ácido p-cumárico, rutina hidrato, ácido vanílico, epicatequín, ácido t-cinámico, ácido gálico y ácido salicílico. El contenido fenólico en las accesiones fue cinco veces más alto que en los genotipos cultivados.
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Hale, A.L., Reddivari, L., Nzaramba, M.N. et al. Interspecific Variability for Antioxidant Activity and Phenolic Content Among Solanum Species. Am. J. Pot Res 85, 332–341 (2008). https://doi.org/10.1007/s12230-008-9035-1
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DOI: https://doi.org/10.1007/s12230-008-9035-1