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Immobilization of Carbonic Anhydrase on Chitosan Stabilized Iron Nanoparticles for the Carbonation Reaction

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Abstract

Carbonic anhydrase (CA) has been immobilized on chitosan stabilized iron nanoparticles (CSIN) for the biomimetic carbonation reaction. CSIN was characterized using scanning electron microscope, energy dispersive X-ray, X-ray diffraction spectroscopy, and Fourier transform infrared analysis. The effect of various parameters such as pH, temperature and storage stability, on immobilized CA was investigated using a p-NPA assay. Kinetic parameters of immobilized and free CA (K m and V max values) were also evaluated. The K m and V max for immobilized CA was 1.727 mM and 1.189 μmol min−1 ml−1, respectively, whereas for free enzyme the K m and V max was 1.594 mM and 1.307 μmol min−1 ml−1, respectively. It was observed that the immobilized enzyme had longer storage stability and retained 50 % of its initial activity upto 30 days at room temperature. CA immobilized on CSIN has been used for hydration of CO2, and the results were validated by using a gas chromatographic method. Proof of concept has been established for the biomimetic carbonation reaction. Immobilized CA show reasonably good CO2 sequestration capacity of 21.55 mg of CaCO3/mg of CA as compared to CO2 sequestration capacity of 34.92 mg of CaCO3/mg of CA for free CA respectively, under a limiting concentration of CO2 (14.5 mg of CO2/10 ml).

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Acknowledgements

This work was carried out under the sponsorship of the Department of Biotechnology (DBT) and Supra Institutional project (SIP-16) funded by CSIR, New Delhi. The authors are also thankful to the Director, NEERI, Nagpur for providing research facilities. We are thankful to SAIF-IIT Bombay, India in providing TEM image of CSIN. Four of the authors (Renu Yadav, Meenal Joshi, Snehal Wanjari and Chandan Prabhu) also sincerely acknowledge the Council of Scientific and Industrial Research (CSIR) India for granting the Senior Research Fellowship.

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Authors and Affiliations

  1. Environmental Material Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nehru Marg, Nagpur, 440020, India

    Renu Yadav, Meenal Joshi, Snehal Wanjari, Chandan Prabhu & Sadhana Rayalu

  2. University Department of Biochemistry, RTM Nagpur University, Nagpur, 440010, India

    Swati Kotwal

  3. Department of Microbiology, University of Delhi South Campus, New Delhi, 110021, India

    T. Satyanarayanan

Authors
  1. Renu Yadav
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  2. Meenal Joshi
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  3. Snehal Wanjari
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  4. Chandan Prabhu
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  5. Swati Kotwal
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  6. T. Satyanarayanan
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  7. Sadhana Rayalu
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Correspondence to Sadhana Rayalu.

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Yadav, R., Joshi, M., Wanjari, S. et al. Immobilization of Carbonic Anhydrase on Chitosan Stabilized Iron Nanoparticles for the Carbonation Reaction. Water Air Soil Pollut 223, 5345–5356 (2012). https://doi.org/10.1007/s11270-012-1284-4

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  • DOI: https://doi.org/10.1007/s11270-012-1284-4

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