Abstract
A screen-printed carbon working electrode within a commercially available screen-printed three-electrode assembly was modified by using a composite of multiwalled carbon nanotubes (MWCNT) dispersed in polyethylenimine (PEI) followed by covering with the calf thymus dsDNA layer. Several electrochemical methods were used to characterize the biosensor and to evaluate damage to the surface-attached DNA: square wave voltammetry of the [Ru(bpy)3]2+ redox indicator and mediator of the guanine moiety oxidation, cyclic voltammetry and electrochemical impedance spectroscopy in the presence of the [Fe(CN)6]3−/4− indicator in solution. Due to high electroconductivity and large surface area of MWCNT and positive charge of PEI, the MWCNT–PEI composite is an advantageous platform for the DNA immobilization by the polyelectrolyte complexation and its voltammetric and impedimetric detection. In this respect, the MWCNT–PEI interface exhibited better properties than the MWCNT–chitosan one reported from our laboratory previously. A deep DNA layer damage at incubation of the biosensor in quinazoline solution was found, which depends on the quinazoline concentration and incubation time.
Similar content being viewed by others
References
Labuda J, Fojta M, Jelen F, Paleček E (2006) Encyclopedia of sensors: electrochemical sensors with DNA recognition layer. American Scientific, CA USA
Lucarelli DF, Kicela A, Palchetti I, Marrazza G, Mascini M (2002) Bioelectrochem 58:113–118
Thevenot DR, Toth K, Durst RA, Wilson GS (1999) Pure Appl Chem 71:2333–2348
Labuda J, Bučková M, Heilerová Ľ, Šilhár S, Štepánek I (2003) Anal Bioanal Chem 376:168–173
Ziyatdinova G, Galandova J, Labuda J (2008) Int J Electroanal Sci 3:223–235
Balasubramanian K, Burghard M (2006) Anal Bioanal Chem 385:451–468
He P, Xu Y, Fang Y (2006) Microchim Acta 152:175–186
Rivas GA, Rubianes MD, Rodriguez MC, Ferreyra NF, Luque GL, Pedano ML, Miscoria SA, Parrado C (2007) Talanta 74:291–307
Shie J-W, Yogeswaran U, Chen S-M (2008) Talanta 74:1659–1669
Guo M, Chen J, Liu D, Nie L, Yao S (2004) Bioelectrochemistry 62:29–35
Guo M, Chen J, Nie L, Yao S (2004) Electrochim Acta 49:2637–2643
He P, Bayachou M (2005) Langmuir 21:6086–6092
Wang J, Kawde A-N, Musameh M (2003) Analyst 128:912–916
Gooding JJ (2005) Electrochim Acta 50:3049–3060
Trojanowitz M, Mulchandani A, Mascini M (2004) Anal Letters 37:3185–3204
Zhang J, Gao L (2007) Mater Lett 61:3571–3574
Wang J, Musameh M, Lin Z (2003) J Am Chem Soc 125:2408–2409
Rivas GA, Miscoria SA, Desbrieres J, Barrera GD (2007) Talanta 71:270–275
Galandova J, Ziyatdinova G, Labuda J (2008) Anal Sci 24:711–716
Li J, Liu Q, Liu Y, Liu S, Yao S (2005) Anal Biochem 346:107–114
Bollo S, Ferreyra NF, Rivas GA (2007) Electroanalysis 19:833–840
Wang G, Xu J-J, Chen H-Y (2002) Electrochem Commun 4:506–509
Rubianes MD, Rivas GA (2007) Electrochem Commun 9:480–484
Shim M, Javey A, Kam NWS, Dai H (2001) J Am Chem Soc 123:11512–11513
Herlem G, Lakard B (2004) J Chem Phys 120:9376–9382
Kolasinska M, Krastev R, Warszynski P (2007) J Coll Interface Sci 305:46–56
Arribas AS, Bermejo E, Chicharo M, Zapardiel A, Luque GL, Ferreyra NF, Rivas GA (2007) Anal Chim Acta 596:183–194
Lojou E, Bianco P (2007) Electrochim Acta 52:7307–7314
Labuda J, Ovadekova R, Galandova J (2009) Microchim Acta 164:371–377
Jantová S, Ovádeková R, Letašiová S, Špirková K, Stankovský Š (2005) Folia Microbiologica 50:90–94
Ovadekova R, Labuda J (2006) Curr Top Electrochem 11:21–56
Pejcic B, De Marco R (2006) Electrochimica Acta 51:6217–6229
Jantová S, Letašiová S, Repický A, Ovádeková R, Lakatoš B (2006) Cell Biochem Funct 24:519–530
Jantová S, Letašiová S, Ovádeková R, Mučková M (2006) Neoplasma 53:291–300
Pang D-W, Zhang M, Wang Z-L, Qi Y-P, Cheng J-K, Liu Z-Y (1996) J Electroanal Chem 403:183–188
Forrest ML, Koerber JT, Pack DW (2003) Bioconjugate Chem 14:934–940
Long EC, Barton JK (1990) Acc Chem Res 23:271–273
Hays HCW, Millner PA, Prodromidis MI (2006) Sensors Actuators B 114:1064–1070
Katz E, Willner I (2003) Electroanalysis 15:913–917
Acknowledgments
This work was supported by the Applied Research Project of the Ministry of Education of Slovak Republic (AV/4/0103/06) and Scientific Grant Agency VEGA project (1/0852/08). The authors thank gratefully Assoc. Prof. Š. Stankovský, Assoc. Prof. K. Špirková, and Assoc. Prof. S. Jantová (STU in Bratislava) for the quinazoline preparatives and Dr. G. Ziyatdinova (Kazan State University, Kazan, Russia) for help with the impedimetric measurements.
Author information
Authors and Affiliations
Corresponding author
Additional information
Dedicated to Professor Jan Garaj on the occasion of his 75th birthday
Rights and permissions
About this article
Cite this article
Galandová, J., Ovádeková, R., Ferancová, A. et al. Disposable DNA biosensor with the carbon nanotubes–polyethyleneimine interface at a screen-printed carbon electrode for tests of DNA layer damage by quinazolines. Anal Bioanal Chem 394, 855–861 (2009). https://doi.org/10.1007/s00216-009-2740-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-009-2740-x