Summary
The polymerase chain reaction (PCR) was used to demonstrate proviral DNA of lentiviruses of small ruminants in cultured cells. Primers for the Taq polymerase were selected in the GAG gene of Icelandic maedi-visna virus and POL gene of caprine arthritis-encephalitis (CAE) virus. Using PCR, proviral DNA of CAE virus was detected at 1 day post infection, 4 days beforeviral protein could be demonstrated using a sensitive immunoblotting protocol and 6 days before the appearance of syncytia. Primers derived from the published sequence of CAE virus successfully primed for the synthesis of homologous virus and Icelandic maedi-visna viruss but not for maedi-visna virus isolated in The Netherlands. In contrast, primers derived from the GAG region of Icelandic maedi-visna virus allowed the amplification of DNA of homologous virus, maedi-visna virus isolated in The Netherlands as well as CAE virus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Narayan, O., and Cork, L. C., Rev. infect. Dis.7 (1985) 89.
Adams, P. S., Gogolewski, R. P., Barbet, A. F., and Cheevers, W. P., J. gen. Virol.66 (1985) 1139.
Houwers, D. J., Gielkens, A. L. J., and Schaake, J., Vet. Microbiol.7 (1982) 209.
Houwers, D. J., and Schaake, J. Jr, J. Immun. Meth.98 (1987) 151.
Zanoni, R., Krieg, A., and Peterhans, E., J. clin. Microbiol.27 (1989) 580.
De Boer, G. F., Terpstra, C., and Houwers, D. J., Res. vet. Sci26 (1979) 202.
Sihvonen, L., Acta vet. scand.21 (1980) 689.
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., and Arnheim, N., Science230 (1985) 1350.
Laure, F., Courgnaud, V., Rouzioux, C., Blanche, S., Veber, F., Burgard, M., Jacomet, C., Griscelli, C., and Brechot, C., Lancet (1988) 538.
Loche, M., and Mach, B., Lancet (1988) 418.
Ou, C.-Y., Kwok, S., Mitchell, S. W., Mack, D. H., Sninsky, J. J., Krebs, J. W., Feorino, P., Warfield, D., and Schochetman, G., Science239 (1988) 295.
Rayfield, M., DeCock, K., Heyward, W., Goldstein, L., Krebs, J., Kwok, S., Lee, S., McCormick, J., Moreau, J. M., Odehouri, K., Schochetman, G., Sninsky, J., and Ou, C.-Y., J. infect. Dis.158 (1988) 1170.
Zwahlen, R., Aeschbacher, M., Balcer, T., Stucki, M., Wyder-Walther, M., Weiss, M., and Steck, F., Schweiz, Arch. Tierheilk.125 (1983) 281.
Laemmli, U. K. Nature227 (1970) 680.
Pyper, J. M., Clements, J. e., Molineaux, S. M., and Narayan, O., J. Virol.51 (1984) 713.
Pyper, J. M., Clements, J. E., Gonda, M. A., and Narayan, O., J. Virol.58 (1986) 665.
Sonigo, P., Alizon, M., Staskus, K., Klatzmann, D., Cole, S., Danos, O., Retzel, E., Tiollais, P., Haase, A., and Wain-Hobsen, S., Cell42 (1985) 369.
Chiu, I.-M., Yaniv, A., Dahlberg, J. E., Gazit, A., Skuntz, S. F., Tronick, S. R., and Aaronson, S. A., Nature317 (1985) 366.
Jeffreys, A. J., Wilson, V., Neumann, R., and Keyte, J., Nucleic Acids Res.16 (1988) 10953.
De Boer, G. F., Terpstra, C., and Houwers, D. J., Off. Int. Epiz.89 (1978) 487.
Li, W.-H., Tanimura, M., and Sharp, P. M., Molec. Biol. Evol.5 (1988) 313.
Yaniv, A., Dahlberg, J. E., Tronick, S. R., Chiu, I.-M., and Aaronson, S. A., Virology145 (1985) 340.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zanoni, R., Pauli, U. & Peterhans, E. Detection of caprine arthritis-encephalitis- and maedi-visna viruses using the polymerase chain reaction. Experientia 46, 316–319 (1990). https://doi.org/10.1007/BF01951776
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01951776