Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution
<p>Force-induced visualization with single-nucleotide resolution. (<b>a</b>) Schematic of the method, which contains a magnetic platform P, analyte strand of unknown length A, and ruler strands R. (<b>b</b>) Photo images of using 15, 14, 13, and 12 nt rulers (R15 to R12) to measure a 13 nt target strand A13 (top row) and 12 nt strand A12 (bottom row). (<b>c</b>) Magnetic signals of the four samples shown in b for A13. (<b>d</b>) Microscopic images of the R14 (left) and R13 samples (right) for A13. Scale bar: 50 μm.</p> "> Figure 2
<p>Adjustable mechanical force based on experimental conditions. (<b>a</b>) The required force vs the ratio of [<span class="html-italic">R</span>]/[<span class="html-italic">A</span>]. (<b>b</b>) The required force at various relative concentrations of TAM<sub>10</sub> buffer, denoted as r.</p> "> Figure 3
<p>Probing the different positions of mRNA in Pre and Post ribosome complexes. (<b>a</b>) Probing scheme. (<b>b</b>) Images of Pre and Post after applying 80 pN. (<b>c</b>) Images of higher concentrations of Pre and Post after applying 230 pN.</p> "> Figure 4
<p>Visualization of drug binding specificity. (<b>a</b>) Scheme for FIV. The DNA duplex contains two specific sites for endonucleases, indicated in dark red and green, respectively. (<b>b</b>) Photos of the results after applying 1 pN force. (<b>c</b>) Screening of four drugs against three DNA sequences. 1: daunomycin; 2: netropsin; 3: doxorubicin; 4: epirubicin.</p> "> Figure 5
<p>A dual-purpose device for recording and quantification. (<b>a</b>) Photo of the device. I: sample holder; II: laser pointer; III: voltmeter connected with a photodetector; IV: holder for a smart phone. (<b>b</b>) Correlation between the voltage measured by the photodetector and the number of magnetic particles in the sample well. The fitting was a single-exponential curve. (<b>c</b>) The corresponding photos of the samples. (<b>d</b>) Determining the binding constant of daunomycin using transmission measurements.</p> "> Figure 6
<p>Multiplexed detection using acoustic radiation force. (<b>a</b>) A 15-sample plate placed on a piezo prior to applying ultrasound. (<b>b</b>) The sample plate after applying ultrasound. Each sample well was 4 mm long and 2 mm wide.</p> ">
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Principle and Demonstration of FIV
3.2. Application in Probing Ribosome Translocation
3.3. Application in Sequence-Specific Drug Screening
3.4. A device for Recording and Quantification
3.5. Multiplexed Detection Using Acoustic Radiation Force
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Hu, Q.; Jia, H.; Wang, Y.; Xu, S. Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution. Sensors 2023, 23, 7762. https://doi.org/10.3390/s23187762
Hu Q, Jia H, Wang Y, Xu S. Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution. Sensors. 2023; 23(18):7762. https://doi.org/10.3390/s23187762
Chicago/Turabian StyleHu, Qiongzheng, Haina Jia, Yuhong Wang, and Shoujun Xu. 2023. "Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution" Sensors 23, no. 18: 7762. https://doi.org/10.3390/s23187762