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Article

The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7

by
Shun Wang
1,2,
Jiufeng Xie
3,
Min Jiang
3,
Keke Chang
1,2,
Ruipeng Chen
1,2,
Liuzheng Ma
1,2,
Juanhua Zhu
1,
Qingqian Guo
1,2,
Haifeng Sun
1,2 and
Jiandong Hu
1,2,*
1
Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 45002, China
2
State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 45002, China
3
College of life sciences, Henan Agricultural University, Zhengzhou 45002, China
*
Author to whom correspondence should be addressed.
Sensors 2016, 16(11), 1856; https://doi.org/10.3390/s16111856
Submission received: 12 September 2016 / Revised: 18 October 2016 / Accepted: 1 November 2016 / Published: 4 November 2016
(This article belongs to the Section Biosensors)
Browse Figures
Figure 1
<p>The schematic diagram of the portable surface plasmon resonance (SPR) bioanalyzer. (<b>a</b>): Principle of surface plasmon resonance biosensing platform; (<b>b</b>): Schematic representation of construction of the portable SPR bioanalyzer.</p> "> Figure 2
<p>Schematic diagram of the procedure of <span class="html-italic">Escherichia coli</span> O157:H7 biomolecular recognition membrane. (1) A 3-MPA SAM was first attached to the biosensor surface; (2) The <span class="html-italic">E. coli</span> O157:H7 antibody molecules were immobilized on the sensor surface.</p> "> Figure 3
<p>The response unit obtained from the functionalization of biomolecular recognition membrane.</p> "> Figure 4
<p>SPR sensorgrams generated from the detection of the positive <span class="html-italic">E. coli</span> O157:H7 sample.</p> "> Figure 5
<p>The relationship between different concentrations of <span class="html-italic">E. coli</span> O157:H7 and the SPR response signals.</p> ">
Versions Notes

Abstract

:
The purpose of this study was to develop a portable surface plasmon resonance (SPR) bioanalyzer for the sensitive detection of Escherichia coli O157:H7 in comparison with an enzyme-linked immunosorbent assay (ELISA). The experimental setup mainly consisted of an integrated biosensor and a homemade microfluidic cell with a three-way solenoid valve. In order to detect Escherichia coli O157:H7 using the SPR immunoassay, 3-mercaptopropionic acid (3-MPA) was chemisorbed onto a gold surface via covalent bond for the immobilization of biological species. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were used as crosslinker reagents to enable the reaction between 3-MPA and Escherichia coli O157:H7 antibodies by covalent –CO–NH– amide bonding. The experimental results were obtained from the Escherichia coli O157:H7 positive samples prepared by 10-, 20-, 40-, 80-, and 160-fold dilution respectively, which show that a good linear relationship with the correlation coefficient R of 0.982 existed between the response units from the portable SPR bioanalyzer and the concentration of Escherichia coli O157:H7 positive samples. Moreover, the theoretical detection limit of 1.87 × 103 cfu/mL was calculated from the positive control samples. Compared with the Escherichia coli O157:H7 ELISA kit, the sensitivity of this portable SPR bioanalyzer is four orders of magnitude higher than the ELISA kit. The results demonstrate that the portable SPR bioanalyzer could provide an alternative method for the quantitative and sensitive determination of Escherichia coli O157:H7 in field.

1. Introduction

For the prevention of infection, illness, and economic loss, rapid detection of pathogenic microorganisms in food and water has been a challenge for decades. Most food poisoning cases are caused by microbial pollution, where Escherichia coli (E. coli) is one of the most common pathogenic bacteria and brings great harm to human body health. Furthermore, as is widely known to the public, E. coli O157:H7 can cause human enteritis and hemorrhagic diarrhea. Annual economic losses due to human norovirus, E. coli O157:H7, have been estimated to be $0.27 billion [1]. Hence, some novel, rapid, and more practical techniques for the identification of bacterial contamination in food are urgently required.
Recently, a variety of techniques have been developed for the identification of E. coli O157:H7, including serological separation, bacteria morphology, and flow cytometry [2,3]. Although these techniques have been successfully utilized to detect the E. coli O157:H7 in food samples, some of these methods require sophisticated and expensive instruments, or radioactive and expensive chemicals, or involve a tedious procedure. Moreover, it is difficult for some methods to be used for rapid detection and quantification of the E. coli O157:H7 at low concentrations due to the complicated preparation. For instance, the method of microbiological culture needs to take one or more enrichment cycles and work up to 36 h to obtain the measurement results. Therefore, it is of considerable interest to explore a low-cost approach for the identification of E. coli O157:H7. Serological classification is used for the detection of E. coli O157:H7 mainly by using a monoclonal antibody to establish an enzyme-linked immunosorbent assay (ELISA) and the latex coagulation experiment [4]. Immunologic-based biosensor technologies, such as amperometricimmuno sensors [4,5,6,7], electrochemical detection [8,9], piezoelectric biosensors [10,11], electrical impedance biosensors [12], and immunomagnetic biosensors [13] have been widely applied for the detection of bacteria. With the rapid development in molecular biology, the polymerase chain reaction (PCR) has been widely used to evaluate E. coli O157:H7 without environmental influences [14,15,16]. A successful combination of physics and biology, the low cost surface plasmon resonance (SPR) biosensor has been developed in recent years. With the advantages of label-free, non-intrusive, real-time biomolecule detection, SPR biosensors have been widely applied in life science, drug development, environmental monitoring, and food safety [17,18,19]. An increasing number of research groups have been dedicated to studying the detection of the pathogens, such as Staphylococcus aureus, bacteriophages, and E. coli O157:H7 using quartz crystal microbalance (QCM) and the electrochemical method [20,21,22,23,24]. Compared with the QCM and the electrochemical method, SPR has some prominent advantages: firstly, it can achieve real time monitoring of biomolecular interactions; secondly, it can determine both kinetics parameters. However, an enzyme-linked immunosorbentassay (ELISA) kit is one frequently used method to detect E. coli O157:H7 in situ, which combines the specificity of antibodies with the sensitivity of simple enzyme assays by using antibodies or antigens coupled to an easily-assayed enzyme. However, in most cases, such as meat and dairy products, the concentration of E. coli O157:H7 is too low, which frequently leads to a false positive result [25]. To handle this problem, label-free, non-intrusive, real-time biomolecule detection was introduced, which is based on a low-cost SPR biosensor combined with microfluidic cell techniques.
The aim of the present investigation is to develop a portable SPR bioanalyzer to monitor the specific binding reaction between E. coli O157:H7 antigen and antibody. The E. coli O157:H7 antibody was covalently attached on the 3-MPA/Au SPR chip, and the detection of E. coli O157:H7 was conducted. A sensitive SPR bioanalyzer was provided to greatly realize the label-free and real-time E. coli O157:H7 antigen and antibody analysis. To verify the performance of the portable SPR bioanalyzer, the ELISA kit was also used for the detection of E. coli O157:H7. By comparing the experiment results obtained from these two approaches, the fabricated portable SPR bioanalyzer shows great potential for rapid detection of E. coli O157:H7.

2. Materials and Methods

2.1. Materials

An Escherichia coli O157:H7 ELISA kit and the positive control sample containing 4.0 × 105 cfu/mL of E. coli O157:H7 were purchased from Biolab Co., Ltd. (Beijing, China). The positive control sample containing 4 × 105 cfu/mL of E. coli O157:H7 was firstly diluted to 4.0 × 104 cfu/mL (10-fold step). Then, the positive control sample containing 4 × 104 cfu/mL of E. coli O157:H7 was serially diluted to 0.25 × 104–2.0 × 104 cfu/mL (2-fold steps) for the measurement.
3-Mercaptopropionic acid (MPA), ethanol amine (Eth), 1-ethyl-(3-3-dimethylaminopro-pyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), phosphate buffer (PBS) (pH 7.4), NaOH, and sodium dodecyl sulfonate (SDS) were purchased from Shanghai General Chemical Reagent Factory (Shanghai, China). The integrated biosensor modular (Spreeta biosensor) manufactured with a 50 nm gold slide bonded to the sensor modules was purchased from Nomadics, Inc. (Stillwater, OK, USA).

2.2. Methods

The surface plasmon wave (SPW) at the metal–dielectric interface, decaying evanescently into both media, is derived from the electron density oscillations caused by a TM (transverse magnetic) or p-polarized light. This TM field intensity makes the surface plasmon in the vicinity of the surface of the metal (<100 nm) very sensitive to the sample mass. The perturbations of the SPW are highly sensitive to the small changes in refractive index (RI). For the SPR bioanalyzer constructed by SPR biosensors using the coupling method of the attenuated total reflection (ATR), the propagation constants of the incident light wave and the SPW along the x-axis are the same (see Figure 1). In this case, the resonance occurs at a particular wavelength. The resonance parameter, either angle or wavelength, depends on the refractive index of the dielectric medium. In SPR biosensors, biomolecular recognition elements (e.g., antibodies) are covalently bound on the Au film surface. If the sample containing target molecules flow through the Au film surface, the target molecules will bind to the biomolecular recognition elements, leading to changes in the refractive index (RI) near the surface of the SPR biosensor. The ability to directly measure interactions in real time can allow researchers to quantitatively determine kinetic parameters, thermodynamics, and concentrations, or to qualitatively characterize relationships between ligands and analytes. The schematic diagram of this portable SPR bioanalyzer is shown in Figure 1; when the divergent polarized incident light beam has the correct incidence angle within TIR, reflection is decreased at the resonance angle. Obviously, the resonance condition depends on the angle of incidence, the wavelength of the light beam, and the dielectric functions of both the metal and the dielectric. The SPR shift in the resonance angle is directly proportional to changes in the refractive index at the Au surface. Based on this relationship, the SPR shift indicted with a sharp dip position is used as the assessment of the dielectric constant or refractive index changed on the Au surface. The photoelectric signals corresponding to the SPR shift were acquired via the linear charge-coupled device (CCD) array and used to establish a SPR response curve. In this experiment, the data digital-filtering algorithms were used to process the photoelectric signals from the linear CCD array.
A microfluidic cell, including a 3-way solenoid valve, was used to transfer samples over the biosensing chip. From Figure 1b, the homemade three-channel microfluidic cell (2.5 µL) was fabricated to irrigate three kinds of different samples controlled by the 3-way solenoid valve over the surface of the biosensing chip according to the practical demands. Fluidics are made up of 1/16 inch OD (outside diameter) fluorinated ethylene propylene tubing flowing into selected channels. The inlets and the outlets were formed by placing a thin PMMA (polymethyl methacrylate) plate over the PDMS (polydimethylsiloxane) gasket with six separate 1/16 inch holes drilled through, allowing access to each channel. Before the SPR bioanalyzer was used, the biomolecular recognition membrane was immobilized. The positions of the resonant dip on the linear CCD were shifted by changing the refractive index of the biological sample solution, which flowed through the microfluidic cell.

2.3. Immobilization of the Escherichia coli O157:H7 Antibody on the Au Film Surface

By using an ultrasonic cleaner, the Au film surface of the integrated SPR biosensor was cleaned with 4% SDS for 5 min, and it was then rinsed with deionized water and dried with N2 in advance. After that, the integrated SPR biosensor was firmly clamped with the Au film and then connected to the circuit board for the acquisition of SPR response signals. Generally, a self-assembled monolayer (SAM) was first attached on the surface of the sensor utilizing the covalent bonds between the thiol group and gold, forming a Au–S bond. In our experiment, a 3-MPA SAM was first attached to the biosensor surface to form the 3-MPA/Au SPR chip. Then, the carboxyl groups of 3-MPA molecules were released to interact with amino groups of the E. coli O157:H7 antibody. Thus, E. coli O157:H7 antibody molecules were easily immobilized on the treated sensor surface. The functionalization of the biomolecular recognition membrane is illustrated in Figure 2.

3. Results and Discussion

3.1. Evaluation of the Response Units from the SPR Biosensor for the Biomolecular Recognition Membrane

The detailed immobilization procedure of the E. coli O157:H7 antibody is described as follows. (1) A pH 7.4 PBS solution flowed to the surface of the Au film at a flow rate of 30 μL/min to obtain a constant baseline. Subsequently, 3-MPA (0.01 mol/L) was injected after the response signals began to emerge steadily with a line. Meanwhile, the response curve was formed by calculating the response signals from the circuit board, where the circuit board was used to acquire the SPR response signals and then display the signals on the touch-screen in real time. The PBS was blocked for a while and injected into the microfluidic cell again to keep the response signal still stable, which indicated that the carboxyl group layer was firmly immobilized on the Au film. (2) To activate the 3-MPA/Au SPR chip, the mixed solution of 0.4 mol/L EDC and 0.1 mol/L NHS with a mixture ratio of 1:1 (1:1, V/V) was injected. The activation time, which is defined as the time that it takes for the covalent attachment of the E. coli O157:H7 antibody on the surface of the EDC-NHS to activate 3-MBA/Au SPR chip, approximately one hour. (3) The 50-fold diluted antibody of E. coli O157:H7 was injected into the microfluidic cell, and the response signals were monitored in real time until the occurrence of the stable response signals. Afterwards, the PBS flowed through the surface of the SPR biosensor until the response signals steadily recurred, which indicated that the E. coli O157:H7 antibodies were firmly immobilized on the Au film. (4) A 1 mol/L ethanolamine solution was injected to block the carboxyl spots that were not bound with the E. coli O157:H7 antibodies on the surface of the Au film.
The pH 7.4 PBS solution should be injected to obtain a constant baseline from the three SPR channels prior to the data acquisition of the biological samples. The response units (RUs) of the biomolecular recognition membrane are clearly shown in Figure 3. First, the MPA solution was injected to cause the –SH group to couple with the Au film deposited on the surface of the biosensor completely in order to immobilize the antibodies of E. coli O157:H7 (see Phase A in Figure 3). A background of 3000 was automatically set by the operating software after system initialization. A normal calibration method was used to convert the delta response unit ΔRU (ΔRU, the response unit of baseline, was subtracted by the current response unit, promptly), which was obtained from the PBS flowed through the Au film surface of the SPR bioanalyzer. After that, the resolution was improved by avoiding the calculation of the very small number. Here, it is equivalent to magnify the value of RU by 3000 times [26]. The delta response unit signal obtained from the binding of Au film with MPA was 457 RU after the Au film surface was washed by PBS. The mixture of EDC and NHS was injected through the Au film surface to activate the carboxyl groups of the self-assembled monolayer. During this activation process, a large amount of bubbles occurred, resulting in large fluctuations in the curve, as shown in Phase B in Figure 3. The response units (RUs) began to increase after the surface of the 50 nm Au film was washed with PBS. In Phase C in Figure 3, the E. coli O157:H7 antibody containing –NH2 groups was immobilized via covalent–CO–NH– amide bonding after the carboxyl groups on MPA were activated by the mixed solution of EDC and NHS. The RUs indicated with the baseline were 3602 before the antibodies of E. coli O157:H7 were fixed on the Au film surface of the integrated SPR biosensor, while the values of the RU reached 4461 at the equilibrium plateau after the E. coli O157:H7 antibodies were fixed on the Au surface of the biosensor; then, the values of the RU reached a stable level of 4098 after being washed by PBS. In this process, the RU increased after the immobilization antibodies of E. coli O157:H7 with an ΔRU of 496 RU. Sequentially, Eth was injected to seal off the carboxyl (see Phase D in Figure 3). It can be seen from Figure 3 that the ΔRU increased with the process of immobilization, which demonstrates that the biomolecular recognition membrane was prepared successfully.

3.2. Samples Analysis of E. coli O157:H7

For the investigation of sensitivity of the portable SPR bioanalyzer, the positive E. coli O157:H7 control sample was diluted 10-, 20-, 40-, 80-, and 160-fold with corresponding concentrations of 4.0 × 104 cfu/mL, 2.0 × 104 cfu/mL, 1.0 × 104 cfu/mL, 0.5 × 104 cfu/mL, and 0.25 × 104 cfu/mL, respectively. The preparation process is illustrated in the inset of Figure 4. The E. coli O157:H7 antibody/3-MPA/Au SPR chip was regenerated via treatment with 0.1 M NaOH. The small changes in response unit resulted from the nonspecific binding of cells to the surface. This method completely regenerates the Au surface without affecting its surface properties and makes this process more cost-effective in decreasing the nonspecific binding. Thereafter, each of them flowed over the Au film surface of the integrated SPR biosensor. After the measurement on each different concentration, the non-specific reaction needed to be dissociated with PBS (pH = 7.4), and the surface also needed to be regenerated with NaOH (pH = 13) and acetonitrile solution. In Figure 4, the response values decrease when the dilution factor increases. Therefore, it is easy to distinguish the slight differences in terms of concentration. All this conforms to the law of direct detection.
The fitting curve (see Figure 5) was established based on the relationship between delta response units and different E. coli O157:H7 control samples, with known concentrations ranging from 0.25 × 104 cfu/mL to 4 × 104 cfu/mL. The relation is expressed as ∆RU = 0.0093 × C + 106.7265 with the correlation coefficient R of 0.982, where C is the concentration of E. coli O157:H7. Therefore, there is a positive proportional relationship between the value of the response signal ΔRU and the concentrations. The results show that a sensitivity of 2.5 × 103 cfu/mL was obtained from the diluted positive control samples. Moreover, the limit of detection of 1.87 × 103 cfu/mL was calculated from the empirical formula expressed as 3σ/k, where σ is the standard deviation, and k is the slope of the fitting equation.

3.3. Comparison with the E. coli O157:H7ELISA Kit

In order to compare the sensitivity of this portable SPR bioanalyzer, the commercial ELISA kit for on-site detection of E. coli O157:H7 was carefully administered. The designed use of an E. coli O157:H7 ELISA kit as an aid in the detection of E. coli O157:H7, following the same ELISA assay principles, is a qualitative, rapid test for the presumptive qualitative detection of E. coli O157:H7 from a variety of food matrices, including meat and dairy products. In this experiment, the E. coli O157:H7 positive sample from the ELISA kit was diluted 10-, 20-, 40-, 80-, and 160-fold with PBS. Under identical conditions, all dilution samples were measured with the portable SPR bioanalyzer and the E. coli O157:H7 ELISA kit. As shown in Table 1, the results from the portable SPR bioanalyzer are consistent well with the ELISA kit in the range of less than 40-fold dilutions. Moreover, the E. coli O157:H7 positive sample with as low as 160-fold dilutions can still be successfully detected using this portable SPR bioanalyzer. Therefore, the proposed portable SPR bioanalyzer here is fast and highly sensitive in comparison with the E. coli O157:H7 ELISA kit.

4. Conclusions

A portable SPR bioanalyzer for the sensitive detection of E. coli O157:H7 was systematically investigated based on the SPR biosensor by modifying the E. coli O157:H7 antibody on a layer of gold film. Based on the SPR bioanalyzer, a non-destructive, label-free, and real-time monitoring can be achieved. The positive E. coli O157:H7 control experiments were performed by having the diluted positive E. coli with 10-, 20-, 40-, 80-, and 160-fold solutions flow through the SPR bioanalyzer. To obtain the response values through monitoring binding response signal changes between the E. coli O157:H7 antibody and the E. coli O157:H7, the delta RU was substituted into the established standard concentration curve to obtain the concentration that needs to be detected. A linear relationship (the correlation coefficient R of 0.982) between the concentrations of E. coli O157:H7 and RU values was established. In comparison with the traditional molecular biology methods, the newly developed portable SPR bioanalyzer could be used as a stable, precise, and sensitive approach for quantifying E. coli O157:H7 in field.

Acknowledgments

We are grateful for the financial support provided by the National Natural Science Foundation of China (31671581, U1304305), Henan Province Collaborative Innovation Center of Biomass Energy, Henan Province Joint Funding (1321006000073), State Key Laboratory of Wheat and Maize Crop Science Funding (SKL2014ZH-06, 39990026), Project 948 of the Ministry of Agriculture of China (2015-Z45), and the Zhengzhou Science and Technology Program (141PPTGG405).

Author Contributions

All authors contributed extensively to the work in this paper. Shun Wang, Jiandong Hu, and Jiufeng Xie conceived and designed the experiments; Shun Wang, Min Jiang, Jiufeng Xie, and Keke Chang performed the experiments; Shun Wang, Juanhua Zhu analyzed the data; Liuzheng Ma and Qingqian Guo prepared the samples; Haifeng Sun and Qingqian Guo contributed analysis tools; Shun Wang and Jiandong Hu wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. The schematic diagram of the portable surface plasmon resonance (SPR) bioanalyzer. (a): Principle of surface plasmon resonance biosensing platform; (b): Schematic representation of construction of the portable SPR bioanalyzer.
Figure 1. The schematic diagram of the portable surface plasmon resonance (SPR) bioanalyzer. (a): Principle of surface plasmon resonance biosensing platform; (b): Schematic representation of construction of the portable SPR bioanalyzer.
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Figure 2. Schematic diagram of the procedure of Escherichia coli O157:H7 biomolecular recognition membrane. (1) A 3-MPA SAM was first attached to the biosensor surface; (2) The E. coli O157:H7 antibody molecules were immobilized on the sensor surface.
Figure 2. Schematic diagram of the procedure of Escherichia coli O157:H7 biomolecular recognition membrane. (1) A 3-MPA SAM was first attached to the biosensor surface; (2) The E. coli O157:H7 antibody molecules were immobilized on the sensor surface.
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Figure 3. The response unit obtained from the functionalization of biomolecular recognition membrane.
Figure 3. The response unit obtained from the functionalization of biomolecular recognition membrane.
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Figure 4. SPR sensorgrams generated from the detection of the positive E. coli O157:H7 sample.
Figure 4. SPR sensorgrams generated from the detection of the positive E. coli O157:H7 sample.
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Figure 5. The relationship between different concentrations of E. coli O157:H7 and the SPR response signals.
Figure 5. The relationship between different concentrations of E. coli O157:H7 and the SPR response signals.
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Table
Table 1. Comparison of the sensitivity and specificity between the portable SPR bioanalyzer and the E. coli O157:H7 ELISA kit.
Table 1. Comparison of the sensitivity and specificity between the portable SPR bioanalyzer and the E. coli O157:H7 ELISA kit.
Diluted FactorsOriginal Positive Sample10204080160
SPR bioanalyzer++++++
ELISA kit++++−−
+: indicating positive sign; −: indicating negative sign.

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Wang, S.; Xie, J.; Jiang, M.; Chang, K.; Chen, R.; Ma, L.; Zhu, J.; Guo, Q.; Sun, H.; Hu, J. The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7. Sensors 2016, 16, 1856. https://doi.org/10.3390/s16111856

AMA Style

Wang S, Xie J, Jiang M, Chang K, Chen R, Ma L, Zhu J, Guo Q, Sun H, Hu J. The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7. Sensors. 2016; 16(11):1856. https://doi.org/10.3390/s16111856

Chicago/Turabian Style

Wang, Shun, Jiufeng Xie, Min Jiang, Keke Chang, Ruipeng Chen, Liuzheng Ma, Juanhua Zhu, Qingqian Guo, Haifeng Sun, and Jiandong Hu. 2016. "The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7" Sensors 16, no. 11: 1856. https://doi.org/10.3390/s16111856

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