Jasmonate Signalling and Defence Responses in the Model Legume Medicago truncatula—A Focus on Responses to Fusarium Wilt Disease
<p>JA-inducible gene expression following <span class="html-italic">F. oxysporum</span> infection. <span class="html-italic">M. truncatula</span> A17 seedlings were inoculated with <span class="html-italic">F. oxysporum</span> (<span class="html-italic">Fom</span>-5190a) and root and shoot tissues harvested separately at 1, 2, 4 and 7 days post inoculation (dpi). Gene expression values were determined relative to the internal control <span class="html-italic">Beta-tubulin</span> gene for each mock or <span class="html-italic">Fusarium</span> treated sample. Values shown are fold-inductions in <span class="html-italic">Fusarium</span> treated samples relative to mock treated samples at the same time-point from the average of eight pooled plants.</p> "> Figure 2
<p>Susceptibility of JA-related <span class="html-italic">Tnt1</span>-insertion mutants to <span class="html-italic">F. oxysporum</span>. Seedlings were inoculated with <span class="html-italic">F. oxysporum</span> (<span class="html-italic">Fom</span>-5190a) and survival rates monitored over 35-days. Values are averages ± SE (n = 10). The <span class="html-italic">Tnt1</span>-insertion mutants are in the R108 background and their details noted in <a href="#plants-05-00011-t002" class="html-table">Table 2</a>. A17 is included as a resistant control. Asterisks indicate values that are significantly different (** <span class="html-italic">p</span> < 0.01, * <span class="html-italic">p</span> < 0.05, Student’s t-test) from R108. Similar results were obtained in an independent experiment.</p> ">
Abstract
:1. Introduction
1.1. Pathogen Background
1.2. Host Jasmonate Signaling and F. oxysporum Disease Outcome
1.3. Fusarium Wilt of Legumes
2. Genomic and Transcriptomic M. truncatula Resources
Utilizing Gene Expression Resources to Study Medicago Responses to F. oxysporum Infection
Term | Description | FDR |
---|---|---|
GO:0044255 | cellular lipid metabolic process | 1.40E-17 |
GO:0019748 | secondary metabolic process | 1.40E-15 |
GO:0008610 | lipid biosynthetic process | 1.40E-15 |
GO:0032787 | monocarboxylic acid metabolic process | 2.40E-14 |
GO:0050896 | response to stimulus | 4.80E-14 |
GO:0009607 | response to biotic stimulus | 2.60E-13 |
GO:0010033 | response to organic substance | 2.30E-11 |
GO:0016053 | organic acid biosynthetic process | 3.10E-11 |
GO:0046394 | carboxylic acid biosynthetic process | 3.10E-11 |
GO:0006629 | lipid metabolic process | 3.80E-11 |
GO:0043436 | oxoacid metabolic process | 4.80E-11 |
GO:0006082 | organic acid metabolic process | 4.80E-11 |
GO:0019752 | carboxylic acid metabolic process | 4.80E-11 |
GO:0042180 | cellular ketone metabolic process | 4.80E-11 |
GO:0006720 | isoprenoid metabolic process | 5.20E-11 |
GO:0006631 | fatty acid metabolic process | 7.20E-11 |
GO:0006633 | fatty acid biosynthetic process | 1.90E-10 |
GO:0009719 | response to endogenous stimulus | 2.30E-10 |
GO:0042221 | response to chemical stimulus | 7.20E-10 |
GO:0008299 | isoprenoid biosynthetic process | 1.10E-09 |
3. Genetic/Mutant Resources Available in M. truncatula
Utilizing M. truncatula Mutant Resources to Dissect Host JA-Responses under F. oxysporum Infection
Putative Mutant | Medtr ID | TC | Insertion Line | Gene Function | Arabidopsis Homologue |
---|---|---|---|---|---|
Mtlox1 | Medtr8g018430 | TC132688 | NF0217 insertion Ase8 | 9S-lipoxygenase | AT1G55020 LOX1 AT3G22400 LOX5 |
Mterf1 | Medtr4g100380 | TC114237 | NF1858 insertion 26 | MtERF1-A transcription factor | AT4G17500 ATERF-1 |
4. Experimental Section
4.1. Plant Growth Conditions
4.2. Pathogen Assays
4.3. qRT-PCR
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Thatcher, L.F.; Gao, L.-L.; Singh, K.B. Jasmonate Signalling and Defence Responses in the Model Legume Medicago truncatula—A Focus on Responses to Fusarium Wilt Disease. Plants 2016, 5, 11. https://doi.org/10.3390/plants5010011
Thatcher LF, Gao L-L, Singh KB. Jasmonate Signalling and Defence Responses in the Model Legume Medicago truncatula—A Focus on Responses to Fusarium Wilt Disease. Plants. 2016; 5(1):11. https://doi.org/10.3390/plants5010011
Chicago/Turabian StyleThatcher, Louise F., Ling-Ling Gao, and Karam B. Singh. 2016. "Jasmonate Signalling and Defence Responses in the Model Legume Medicago truncatula—A Focus on Responses to Fusarium Wilt Disease" Plants 5, no. 1: 11. https://doi.org/10.3390/plants5010011