Effect of Earthing Mats on Sleep Quality in Rats
<p>Effect of earthing mat on sleep architecture. Changes in the percentage of wake time (<b>A</b>), REM sleep (<b>B</b>), NREM sleep (<b>C</b>), and total sleep (<b>D</b>) during the dark phase are depicted in the earthing mat-exposed groups. The data represent the mean ± SEM of the percentage of time spent in the sleep–wake state. *** <span class="html-italic">p</span> < 0.001, * <span class="html-italic">p</span> < 0.05 vs. Nor, ### <span class="html-italic">p</span> < 0.001, ## <span class="html-italic">p</span> < 0.01 vs. A21-D; one-way ANOVA followed by Tukey. ● Nor, ■ A-7D,▲ A-21D,▼EM.</p> "> Figure 2
<p>Impact of earthing mat on orexin-positive cells in the LH. (<b>A</b>) Photomicrographs illustrating orexin-positive cells in the LH. The dashed circles indicate the LH region. (<b>B</b>) Quantification of orexin-positive cells in the LH. *** <span class="html-italic">p</span> < 0.01 vs. Nor; ### <span class="html-italic">p</span> < 0.05 vs. A-21D; one-way ANOVA followed by Tukey’s test.</p> "> Figure 3
<p>Impact of earthing mat on SOD-positive cells in the LH. (<b>A</b>) Photomicrographs illustrating orexin-positive cells in the LH. (<b>B</b>) Quantification of SOD-positive cells in the LH. *** <span class="html-italic">p</span> < 0.01 vs. Nor; one-way ANOVA followed by Tukey’s test.</p> "> Figure 4
<p>Animal groups and treatments in the experimental design of this study.</p> ">
Abstract
:1. Introduction
2. Results
2.1. Effect of Earthing Mat on EEG Sleep Architecture and Profile
2.2. Effect of Earthing Mat on the Number of Orexin Neurons in the LH
2.3. Effect of Earthing Mat on the Number of SOD-Positive Cells in the LH
3. Discussion
4. Materials and Methods
4.1. Animals and Grouping
4.2. Earthing Mat
4.3. EEG Surgery
4.4. Methodology of EEG Recording
4.5. Staining of the LH
4.6. Data Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Ye, M.; Jeong, W.; Yu, H.-j.; Kim, K.-r.; Rhie, S.J.; Kim, Y.; Kim, J.; Shim, I. Effect of Earthing Mats on Sleep Quality in Rats. Int. J. Mol. Sci. 2024, 25, 9791. https://doi.org/10.3390/ijms25189791
Ye M, Jeong W, Yu H-j, Kim K-r, Rhie SJ, Kim Y, Kim J, Shim I. Effect of Earthing Mats on Sleep Quality in Rats. International Journal of Molecular Sciences. 2024; 25(18):9791. https://doi.org/10.3390/ijms25189791
Chicago/Turabian StyleYe, Minsook, Woojin Jeong, Hyo-jeong Yu, Kyu-ri Kim, Sung Ja Rhie, Yongsuk Kim, Jiyoun Kim, and Insop Shim. 2024. "Effect of Earthing Mats on Sleep Quality in Rats" International Journal of Molecular Sciences 25, no. 18: 9791. https://doi.org/10.3390/ijms25189791