Integration of Phosphoproteomics and Transcriptome Studies Reveals ABA Signaling Pathways Regulate UV-B Tolerance in Rhododendron chrysanthum Leaves
<p>Transcriptome analysis of <span class="html-italic">R. chrysanthum</span> under UV-B exposure. (<b>A</b>) The number of DEGS in the transcriptome; (<b>B</b>) PCA of differential genes; (<b>C</b>) KEGG enrichment of differential genes (The number on the edge of each bar represents the number of DEGs).</p> "> Figure 2
<p>Response of the phytohormone signaling pathway to UV-B radiation in <span class="html-italic">R. chrysanthum</span>. (<b>A</b>) Heatmaps depict transcript expression profiles associated with phytohormone signaling pathways under UV-B irradiation. A pink box indicates a gene that is upregulated, while a white box indicates a gene that is downregulated; (<b>B</b>) ABA signaling pathway transcript expression profile. An orange box represents genes that have been upregulated, while a green box represents genes that have been downregulated. (The value is the log2 fold change (log2(FC)) of each gene.).</p> "> Figure 3
<p>Phosphorylation proteome analysis of <span class="html-italic">R. chrysanthum</span> under UV-B irradiation. (<b>A</b>) Classification of differentially phosphorylated sites; (<b>B</b>) Number of differentially phosphorylated proteins and sites; (<b>C</b>) GO classification of differentially phosphorylated protein.</p> "> Figure 4
<p>Three-dimensional homologous model of phosphorus protein in response to UV-B irradiation related to ABA signaling of <span class="html-italic">R. chrysanthum</span>. (<b>A</b>) ABF2; (<b>B</b>) SnRK2. The areas with increased phosphorylation levels are marked with red circles.</p> "> Figure 5
<p>Study on phosphorylated protein and gene levels in ABA signal transduction pathway of <span class="html-italic">R. chrysanthum</span> irradiated by UV-B. DEGs are filtered using log2 FPKM (transcript fragments per kilobase per million mapped reads). Genes that are downregulated or upregulated are indicated by green and red text, respectively. The green and yellow boxes represent phosphorylated downregulated and upregulated proteins, respectively.</p> "> Figure 6
<p>Effects of different treatments on stomata of <span class="html-italic">R. chrysanthum</span>. (<b>A</b>) Stomatal morphology changes under different treatments; (<b>B</b>) Stomatal area; (<b>C</b>) Stomatal length; (<b>D</b>) Stomatal width.</p> ">
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatment
2.2. Determination of Stomatal Characteristics
2.3. RNA-Seq Library Construction and Sequencing
2.4. De Novo Assembly and Sequence Annotation
2.5. Differential Expression Analysis
2.6. Protein Extraction and Digestion
2.7. Phosphopeptide Enrichment
2.8. LC-MS/MS Analysis and Database Search
2.9. Bioinformatics
3. Results
3.1. Transcriptomic Analysis of R. chrysanthum under UV-B Stress
3.2. Phosphorylated Proteomics Analysis of R. chrysanthum under UV-B Stress
3.3. Three-Dimensional Structure Modeling of UV-B Stress-Responsive Phosphoproteins
3.4. Transcriptomic and Phosphorylated Proteomic Interaction Analysis to Explore the Response of R. chrysanthum to UV-B Stress
3.5. Effect of Exogenous ABA on Stomata of R. chrysanthum after UV-B Stress
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sun, Q.; Zhou, X.; Yang, L.; Xu, H.; Zhou, X. Integration of Phosphoproteomics and Transcriptome Studies Reveals ABA Signaling Pathways Regulate UV-B Tolerance in Rhododendron chrysanthum Leaves. Genes 2023, 14, 1153. https://doi.org/10.3390/genes14061153
Sun Q, Zhou X, Yang L, Xu H, Zhou X. Integration of Phosphoproteomics and Transcriptome Studies Reveals ABA Signaling Pathways Regulate UV-B Tolerance in Rhododendron chrysanthum Leaves. Genes. 2023; 14(6):1153. https://doi.org/10.3390/genes14061153
Chicago/Turabian StyleSun, Qi, Xiangru Zhou, Liping Yang, Hongwei Xu, and Xiaofu Zhou. 2023. "Integration of Phosphoproteomics and Transcriptome Studies Reveals ABA Signaling Pathways Regulate UV-B Tolerance in Rhododendron chrysanthum Leaves" Genes 14, no. 6: 1153. https://doi.org/10.3390/genes14061153