Optimization for Bone Samples Embedded in Methyl Methacrylate

Original

Applied Molecular and Cellular Medicine Institute (IMMCA), Conicet-unt-siprosa">María F Moreno García, Alfredo Martin, Shigeko Fushimi, Sara Feldman, Nina F Pastorino, Jorge N Juárez, María V Jammal, Applied Molecular and Cellular Medicine Institute (IMMCA), Conicet-unt-siprosa">Liliana R Missana

Author information

María F Moreno García
Experimental Pathology, Diagnostic & Tissue Engineering Laboratory, Oral Pathology Department, Dental School, Tucumán University
Applied Molecular and Cellular Medicine Institute (IMMCA), Conicet-unt-siprosa
Alfredo Martin
Surgical Techniques Department, Agronomy and Animal Husbandry School, Tucumán University
Shigeko Fushimi
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Sara Feldman
Osteoarticular Biology, Tissue Engineering and Emerging Therapies Laboratory (LABOATEM), Medical Sciences School, Rosario University
Nina F Pastorino
Experimental Pathology, Diagnostic & Tissue Engineering Laboratory, Oral Pathology Department, Dental School, Tucumán University
Jorge N Juárez
Experimental Pathology, Diagnostic & Tissue Engineering Laboratory, Oral Pathology Department, Dental School, Tucumán University
María V Jammal
Experimental Pathology, Diagnostic & Tissue Engineering Laboratory, Oral Pathology Department, Dental School, Tucumán University
Liliana R Missana
Experimental Pathology, Diagnostic & Tissue Engineering Laboratory, Oral Pathology Department, Dental School, Tucumán University
Applied Molecular and Cellular Medicine Institute (IMMCA), Conicet-unt-siprosa

Keywords: Bone, Fast decalcification, Large samples, Methyl methacrylate, Temperature

JOURNAL FREE ACCESS

2022 Volume 31 Issue 3 Pages 181-186

DOI https://doi.org/10.2485/jhtb.31.181

Details

Abstract

Our aim was to update a standard technique for embedding bone samples, with or without implants, using methyl methacrylate (MMA) to obtain reliable optical microscopy images from mineralized bone tissues and bone-implant interfaces. In addition, comparative studies were carried out using different temperatures throughout the polymerization process. Twenty-two New Zealand rabbit calvaria and femur bone samples with or without implants were used. The samples were fixed in 10% buffered formalin, dehydrated in an ascending alcohol series, and infiltrated in methyl methacrylate solutions (I, II and III). The specimens were divided into three groups. Groups 1 and 2 were polymerized at 24°C ± 5°C, while Group 3 was polymerized at 60°C. Group 1 and 2 obtained homogeneous, transparent, crystalline polymerization. Group 3 presented multiple bubbles (ø 2 – 3 mm), depressions, folds and even rupture of the vial container. The MMA bone samples polymerized at room temperature, showed good embedded, low hardness index, thin cuts, and effective staining.

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