Abstract
Naturally occurring 20-hydroxyecdysone is an important anabolic ecdysteroid. A simple thin-layer chromatography method to quantitate 20-hydroxyecdysone in methanolic extract of the whole plant material of Sida rhombifolia L. was developed. This method was successfully applied for quantitative evaluation of dietary supplements. The separation was achieved on glass TLC plates coated with silica gel 60F254, using chloroform: methanol (8:2 v/v) as developing solvent. Densitometric evaluation of 20-hydroxyecdysone was performed at 250 nm in reflectance/absorbance mode. The calibration was in the range of 200–1,000 ng spot−1 and correlation coefficient for the calibration curve was >0.999. In addition, for six different Sida species unique fingerprints were obtained on the HPTLC plate.
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References
Mabberley DJ (2000) The plant book. Cambridge University Press, Cambridge, p 661
Kirtikar KR, Basu BD (1999) Indian medicinal plants. International Book Distributors, Dehradun, p 310
Jadhav AN, Pawar RS, Avula B, Khan IA (2007) Chem Biodivers (in press)
Burdette WJ (1972) Cancer Res 32:1088–1090
Yoshida S, Kobayashi A (2007) Ecdysteroids as termite control agents. In: Koho JKT (ed) Japan Envirochemicals Ltd, Japan
Syrov VN, Khushbaktova ZA (1996) Dokl Akad Nauk 12:47–50
Syrov VN, Khushbaktova ZA, Nabiev AN (1992) Eksp Klin Farmakol 55:61–65
Azizov AP, Seifulla RD, Chubarova AV (1997) Eksp Klin Farmakol 60:47–48
Syrov VN, Nasyrova SS, Khushbaktova ZA (1997) Eksp Klin Farmakol 60:41–44
Syrov VN, Saatov Z, Sagdullaev SS, Mamatkhanov AU (2001) Pharm Chem J 35:667–671
Bathori M, Pongracz Z (2005) Curr Med Chem 12:153–172
Koll K, Reich E, Blatter A, Veit M (2003) J AOAC Int 86:909–915
Lafont R, Porter CJ, Williams E, Read H, Morgan ED, Wilson ID (1993) J Planar Chromatogr Mod TLC 6 55:421–424
Wilson ID (1988) J Chromatogr 438:419–422
Witherow L, Thorp RJ, Wilson ID, Warrander A (1990) J Planar Chromatogr Mod TLC 3:169–172
Darwish FMM, Reinecke MG (2003) Phytochemistry 62:1179
Dinan L, Bourne P, Whiting P (2001) Phytochem Anal 12:110
Pandit SS, Naik SD, Jathar VS, Kulkarni AB (1976) Indian J Chem 14B:907
Rao RVK, Satyanarayana T, Rao BVK (1984) Fitoterapia 55:249
Yao C, Xu Y (2000) Yunnan Zhi Wu Yan Jiu 22:503
Bathori M, Hunyadi A, Janicsak G, Mathe I (2004) J Planar Chromatogr Mod TLC 17:335–341
Bathori M, Mathe I, Praszna L, Rischak K, Kalasz H (1996) J Planar Chromatogr Mod TLC 9:264–268
Acknowledgments
This research is funded in part by “Botanical Dietary Supplements: Science-Base for Authentication” funded by Food and Drug Administration grant number FD-U-002071–06. Authors are thankful to Julie Mikell for her suggestions.
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Jadhav, A.N., Rumalla, C.S., Avula, B. et al. HPTLC Method for Determination of 20-Hydroxyecdysone in Sida rhombifolia L. and Dietary Supplements. Chroma 66, 797–800 (2007). https://doi.org/10.1365/s10337-007-0407-3
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DOI: https://doi.org/10.1365/s10337-007-0407-3