Abstract
MetaboAnalyst is an integrated web-based platform for comprehensive analysis of quantitative metabolomic data. It is designed to be used by biologists (with little or no background in statistics) to perform a variety of complex metabolomic data analysis tasks. These include data processing, data normalization, statistical analysis and high-level functional interpretation. This protocol provides a step-wise description on how to format and upload data to MetaboAnalyst, how to process and normalize data, how to identify significant features and patterns through univariate and multivariate statistical methods and, finally, how to use metabolite set enrichment analysis and metabolic pathway analysis to help elucidate possible biological mechanisms. The complete protocol can be executed in ∼45 min.
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Acknowledgements
We thank the Canadian Institutes for Health Research (CIHR) and the Alberta Ingenuity Fund (AIF; now part of Alberta Innovates—Technology Futures) for financial support.
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J.X. and D.S.W. prepared and tested the protocol and wrote the article.
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Xia, J., Wishart, D. Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst. Nat Protoc 6, 743–760 (2011). https://doi.org/10.1038/nprot.2011.319
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DOI: https://doi.org/10.1038/nprot.2011.319
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