Abstract
What is the nature of the vascular architecture in the cortex that allows the brain to meet the energy demands of neuronal computations? We used high-throughput histology to reconstruct the complete angioarchitecture and the positions of all neuronal somata of multiple cubic millimeter regions of vibrissa primary sensory cortex in mouse. Vascular networks were derived from the reconstruction. In contrast with the standard model of cortical columns that are tightly linked with the vascular network, graph-theoretical analyses revealed that the subsurface microvasculature formed interconnected loops with a topology that was invariant to the position and boundary of columns. Furthermore, the calculated patterns of blood flow in the networks were unrelated to location of columns. Rather, blood sourced by penetrating arterioles was effectively drained by the penetrating venules to limit lateral perfusion. This analysis provides the underpinning to understand functional imaging and the effect of penetrating vessels strokes on brain viability.
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Acknowledgements
We thank J. Lee for assistance in the analysis of the surface vasculature, J.D. Driscoll for assistance with the imaging acquisition software, N. Nishimura and C.B. Schaffer for sharing their data logs, S. Chien, W. Denk, P.J. Drew, A.L. Fairhall, B. Friedman, R.D. Frostig, H.J. Karten, H.S. Seung, T.W. Secomb, A.Y. Shih and B. Weber for critical discussions. This work was supported by the Israeli Science Foundation (Bikura fellowship to P.B.) and the US National Institutes of Health (grants EB003832, MH085499, MH072570 and OD006831).
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P.B., D.K. and P.S.T. designed the study. P.B., P.M.K. and P.S.T. carried out the experiments and analyzed and summarized the data with input from J.P.K., D.K. and H.S. D.K. wrote the manuscript.
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Blinder, P., Tsai, P., Kaufhold, J. et al. The cortical angiome: an interconnected vascular network with noncolumnar patterns of blood flow. Nat Neurosci 16, 889–897 (2013). https://doi.org/10.1038/nn.3426
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DOI: https://doi.org/10.1038/nn.3426
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