Abstract
Pluripotential cells are present in a mouse embryo until at least an early post-implantation stage, as shown by their ability to take part hi the formation of chimaeric animals1 and to form teratocarcinomas2. Until now it has not been possible to establish progressively growing cultures of these cells in vitro, and cell lines have only been obtained after teratocarcinoma formation in vivo. We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo. They have a normal karyotype.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Gardner, R. L. & Papaioannou, V. E. in The Early Development of Mammals (eds Balls, M. & Wild, A. E.) 107–132 (Cambridge University Press, 1975).
Stevens, L. C. Devl Biol 21, 364–382 (1970).
Cole, R. J. & Paul, J. in Preimplantation Stages of Pregnancy (eds Wolstenholme, G. E. W. & O'Connor, M.) 82–122 (Churchill, London, 1965).
Sherman, M. I. Cell 5, 343–349 (1975).
Atienza-Samols, S. B. & Sherman, M. I. Devl Biol 66, 220–231 (1978).
Solter, D. & Knowles, B. Proc. natn. Acad. Sci. U.S.A. 72, 5099–5102 (1975).
Evans, M. J. J. Reprod. Pert. 62, 625–631 (1981).
Evans, M. J., Lovell-Badge, R. H., Stern, P. L. & Stinnakre, M.-G. INSERM Symp. 10, 115–129(1979).
McLaren, A. J. Endocr. 50, 515–526 (1971).
Kaufman, M. H. in Progress in Anatomy Vol. 1 (eds Harrison, R. J. & Holmes, R. L.) 1–34 (Cambridge University Press, 1981).
Stern, P. L. et al. Cell 14, 775–783 (1978).
Feizi, T. Blood Transfusion Immunohaemat. 23, 563–577 (1980).
Kapadia, A., Feizi, T. & Evans, M. J. Expl Cell Res. 131, 185–195 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evans, M., Kaufman, M. Establishment in culture of pluripotential cells from mouse embryos. Nature 292, 154–156 (1981). https://doi.org/10.1038/292154a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/292154a0
This article is cited by
-
FOXO1-mediated lipid metabolism maintains mammalian embryos in dormancy
Nature Cell Biology (2024)
-
Comprehensive Review on the Effect of Stem Cells in Cancer Progression
Current Tissue Microenvironment Reports (2024)
-
Stem Cells and Regenerative Strategies for Wound Healing: Therapeutic and Clinical Implications
Current Pharmacology Reports (2024)
-
The impact of induced pluripotent stem cells in animal conservation
Veterinary Research Communications (2024)
-
Mesenchymal stem cells osteogenic differentiation by ZnO nanoparticles and polyurethane bimodal foam nanocomposites
Cell and Tissue Banking (2024)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.