Abstract
Resting cells of Nocardia paraffinaeCBS 255 58 anaerobically converted oleic acid to 10-hydroxystearic acid (optimum activity at 35°C and pH 7). Addition of oleic acid (0.22 mg/ml of medium), as an inducer, improved the hydroxylating activity. The biocatalyst was inactivated by sonication, lyophilization and heating, but kept 65% of its initial activity after five freeze-thawing treatments. This activity appeared to be located either in the cells or bound to the plasmic membrane.
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Latrasse, A., Paitier, S., Lachot, B. et al. Conversion of oleic acid to 10-hydroxystearic acid by Nocardia paraffinae. Biotechnology Letters 19, 715–718 (1997). https://doi.org/10.1023/A:1018315604709
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DOI: https://doi.org/10.1023/A:1018315604709