Abstract
This study highlights, for the first time, the effect of Algerian nettle (Urtica dioïca L.) water extract against some phytopathogens bacteria. Results showed that water extract exhibited high level of total phenol content (261.7 ± 5.1 mg g−1 GAE) and a remarkable antioxidant activity (IC50 = 20.0 ± 0.6 µg mL−1) comparable to Trolox as reference (IC50 = 16.5 ± 0.5 µg mL−1). Moreover, results showed that water extract presented an interesting inhibition of some Gram-positive and Gram-negative phytopathogens’ bacteria. These results highlighted that secondary metabolites from Urtica dioïca could be an alternative pathway to synthetic pesticides for plant diseases control.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.References
Akgül M (2013) Suitability of stinging nettle (Urtica dioica L.) stalks for medium density fiberboards production. Compos Part B Eng 45(1):925–929. https://doi.org/10.1016/j.compositesb.2012.09.048
Andrews W (1992) Manual of food quality control. 4. Rev. 1. Microbiological analysis. Food and Drug Administration, Washington. FAO Food Nutr Pap 14(4 Revis 1):1–338
Aquino O, Mansilla JP, Maria JS (2006) In vitro selection of an effective fungicide against Armillaria mellea and control of white root rot of grapevine in the field. Pest Manag Sci 62:223–228. https://doi.org/10.1002/ps.1149
Augspole I, Duma M, Ozola B, Cinkmanis I (2017) Phenolic profile of fresh and frozen nettle, goutweed, dandelion and chickweed leaves. In: FOODBALT 2017 conference proceeding, pp 36–39. https://doi.org/10.22616/foodbalt.2017.028
Barragan Ferrer D, Venskutonis PR, Talou T, Barragan Ferrer JM, Zebib B, Merah O (2018) Identification and in vitro activity of bioactive compounds extracted from Tussilago farfara L. plant grown in Lithuania and France. Free Radic Antioxid 8(1):40–47. https://doi.org/10.5530/fra.2018.1.7
Barragan-Ferrer D, Venskutonis PR, Talou T, Zebib B, Barragan Ferrer MJ, Merah O (2016) Bioactive compounds and antioxidant properties of Myrrhis odorata deodorized residue leaves extracts from Lithuania and France origins. Pharm Chem J 3(3):43–48
Bodros E, Baley C (2008) Study of the tensile properties of stinging nettle fibres (Urtica dioica). Mater Lett 62(14):2143–2145
Bourgeois C, Leclerc EA, Corbin C, Doussot J, Serrano V, Vanier J-R, Seigneuret J-M, Brand-Williams W, Cuvelier M, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28:25–30. https://doi.org/10.3390/plants8030050
Carvalho AR, Costa G, Figueirinha A, Liberal J, Prior JA, Lopes MC, Batista MT (2017) Urtica spp.: phenolic composition, safety, antioxidant and anti-inflammatory activities. Food Res Int 99:485–494. https://doi.org/10.1016/j.foodres.2017.06.008
Chang H, Cheng Y, Wu C, Chang S, Chang T, Su Y (2008) Antifungal activity of essential oil and its constituents from Calocedrus macrolepis var. formosana Florin leaf against plant pathogenic fungi. Bioresour Technol 99:6266–6270. https://doi.org/10.1016/j.biortech.2007.12.005
Daci-Ajvazi M, Zeneli L, Daci N, Krasniqi A, Ymeri N (2018) Chemical effects and antioxidant responses of Urtica dioica L. extracts growing along highway. Fresenius Environ Bull 27(1):172–179
Dar SA, Ganai FA, Yousuf AR, Balkhi MU, Bhat TM, Sharma P (2013) Pharmacological and toxicological evaluation of Urtica dioica. Pharm Biol 51:170–180. https://doi.org/10.3109/13880209.2012.715172
Di Virgilio N, Papazoglou EG, Jankauskiene Z, Di Lonardo S, Praczyk M, Wielgusz K (2015) The potential of stinging nettle (Urtica dioica L.) as a crop with multiple uses. Ind Crops Prod 68:42–49. https://doi.org/10.1016/j.indcrop.2014.08.012
Dobravalskytė D, Venskutonis PR, Talou T, Zebib B, Merah O, Ragazinskiene O (2013) Antioxidant properties of deodorized extracts of Tussilago farfara L. Rec Nat Prod 7:201–209
Fattahi S, Zabihi E, Abedian Z, Pourbagher R, Motevalizadeh Ardekani A, Mostafazadeh A, Akhavan-Niaki H (2014) Total phenolic and flavonoid contents of aqueous extract of stinging nettle and in vitro antiproliferative effect on Hela and BT-474 cell lines. Int J Mol Cell Med 3:102–107
Field B, Jorden F, Osbourn A (2006) First encounters-deployment of defense-related natural products by plants. New Phytol 172:193–207. https://doi.org/10.1111/j.1469-8137.2006.01863.x
Fletcher J, Bender C, Budowle B, Cobb WTS, Gold E, Ishimaru CA, Luster D, Melcher U, Murch R, Scherm H, Seem RC, Sherwood JL, Sobral BW, Tolin SA (2006) Plant pathogen forensics: capabilities, needs, and recommendations. Microbiol Mol Biol Rev 70(2):450–471. https://doi.org/10.1128/MMBR.00022-05
Gülçin I, Küfrevioǧlu Öİ, Oktay M, Büyükokuroǧlu ME (2004) Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). J Ethnopharmacol 90(2–3):205–215. https://doi.org/10.1016/j.jep.2003.09.028
Horvath Z, Vecsei L (2009) Current medical aspects of pantethine. Nat Stand 62(7–8):220–229. https://doi.org/10.1177/1533210110392944
Jada SR, Matthews C, Saad MS, Hamzah AS, Lajis NH, Stevens M, Stanslas J (2008) Benzylidene derivatives of and rographolide inhibit growth of breast and colon cancer cells in vitro by inducing G1 arrest and apoptosis. Br J Pharmacol 155(5):641–654. https://doi.org/10.1038/bjp.2008.368
Jothy SL, Zuraini Z, Sasidharan S (2011) Phytochemicals screening, DPPH free radical scavenging and xanthine oxidase inhibitiory activities of Cassia fistula seeds extract. J Med Plants Res 5(10):1941–1947
Kataki MS, Murugamani V, Rajkumari A, Singh P, Mehra DA, Yadav RS (2012) Antioxidant, hepatoprotective, and anthelmintic activities of methanol extract of Urtica dioica L. leaves. Pharm Crops 3:38–46
Kordali S, Cakir A, Zengin H, Duru ME (2003) Antifungal activities of the leaves of three Pistacia species grown in Turkey. Fitoterapia 74:164–167
Kregiel D, Pawlikowska E, Antolak H (2018) Urtica spp.: ordinary plants with extraordinary properties. Molecules 23(7):1664. https://doi.org/10.3390/molecules23071664
Kukrić Z, Topalić-Trivunović L, Kukavica B, Matoš S, Pavičić S, Boroja M, Savić A (2012) Characterization of antioxidant and antimicrobial activities of nettle leaves (Urtica dioïca L.). Acta Period Technol 43:257–272
Külcü DB, Gökışık CD, Aydın S (2019) An investigation of antibacterial and antioxidant activity of Nettle (Urtica dioica L.), Mint (Mentha piperita), Thyme (Thymus serpyllum) and Chenopodium album L. plants from Yaylacık Plateau, Giresun, Turkey. Turk J Agri-Food Sci Technol 7(1):73–80. https://doi.org/10.24925/turjaf.v7i1.73-80.2123
Lee HS (2007) Fungicidal property of active component derived from Acorus gramineus rhizome against phytopathogenic fungi. Bioresour Technol 98:1324–1328. https://doi.org/10.1016/j.biortech.2006.05.018
Malik S, Upadhyaya PK, Miglani S (2011) Thiazolidinediones: a plethora of biological load. Int J Pharm Tech Res 3(1):62–75
Mansoori B, Mohammadi A, Hashemzadeh S, Shirjang S, Baradaran A, Asadi M, Doustvandi MA, Baradaran B (2017) Urtica dioïca extract suppresses miR-21 and metastasis-related genes in breast cancer. Biomed Pharm 93:95–102. https://doi.org/10.1016/j.biopha.2017.06.021
Modarresi-Chahardehi A, Ibrahim D, Sulaiman SF, Mousavi L (2012) Screening antimicrobial activity of various extracts of Urtica dioica. Rev Biol Trop 60:1567–1576. https://doi.org/10.15517/rbt.v60i4.2074
NCCLS (2000) National committee for clinical laboratory standards. Performance standards for antimicrobial disc susceptibility tests, 7th edn. Approved Standard M2-A7. NCCLS, Pennsylvania
Nikolova M, Dzhurmanski A (2009) Evaluation of free radical scavening capacity of extracts from cultivated plants. Biotechnol Biotechnol Equip 23(109):111. https://doi.org/10.1080/13102818.2009.10818377
Nunez YO, Salabarria IS, Collado IG, Hernandez-Galan R (2006) The antifungal activity of widdrol and its biotransformation by Colletotrichum gloeosporioides (penz) Penz. and Sacc. and Botrytis cinerea Pers. J Agric Food Chem 54:7517–7521. https://doi.org/10.1021/jf061436m
Ojala T, Remes S, Haansuu P, Vuorela H, Hiltunen R, Haahtela K, Vuorela P (2000) Antimicrobial activity of some coumarin containing herbal plants growing in Finland. J Ethnopharmacol 73:299–305. https://doi.org/10.1016/s0378-8741(00)00279-8
Orčić D, Francišković M, Bekvalac K, Svirčev E, Beara I, Lesjak M, Mimica-Dukić N (2014) Quantitative determination of plant phenolics in Urtica dioïca extracts by high-performance liquid chromatography coupled with tandem mass spectrometric detection. Food Chem 143:48–53. https://doi.org/10.1016/j.foodchem.2013.07.097
Otles S, Yalcin B (2012) Phenolic compounds analysis of root, stalk, and leaves of nettle. Sci World J 2012:564367. https://doi.org/10.1100/2012/564367
Prabavathy VR, Mathivanan N, Sagadevan E, Murugesan K, Lalithakumari D (2006) Self-fusion of protoplasts enhances chitinase production and biocontrol activity in Trichoderma harzianum. Bioresour Technol 97:2330–2334. https://doi.org/10.1016/j.biortech.2005.10.031
Sánchez-Moreno C, Larrauri JA, Saura-Calixto F (1998) A procedure to measure the antiradical efficiency of polyphenols. J Sci Food Agric 76(2):270–276. https://doi.org/10.1002/(SICI)1097-0010(199802)76:2%3c270:AID-JSFA945%3e3.0.CO;2-9
Sánchez-Rangel JC, Benavides J, Heredia JB, Cisneros-Zevallos L, Jacobo-Velázquez DA (2013) The Folin-Ciocalteu assay revisited: improvement of its specificity for total phenolic. Anal Methods 5:5990–5999. https://doi.org/10.1039/c3ay41125g
Sayed-Ahmad B, Hijazi A, Bazzi H, Rammal H, El-Bazzal A, Annan H (2015) Determination of bioactive molecules and antioxidant activity in stinging nettle (Urtica dioïca). J Multidisc Eng Sci Technol 2(10):2753–2758
Shonte TT, de Kock HL (2017) Descriptive sensory evaluation of cooked stinging nettle (Urtica dioïca L.) leaves and leaf infusions: effect of using fresh or oven-dried leaves. South Afr J Bot 110:167–176
Singh R, Dar SA, Sharma P (2012) Antibacterial activity and toxicological evolution of semi purified hexane extract of Urtica dioica leaves. Res J Med Plant 6:123–135. https://doi.org/10.3923/rjmp.2012.123.135
Steffens JJ, Pell EJ, Tien M (1996) Mechanisms of fungicide resistance in phytopathogenic fungi. Curr Opin Biotechnol 7:348–355. https://doi.org/10.1016/S0958-1669(96)80043-7
Tahri A, Yamani S, Legssyer A, Aziz M, Mekhfi H, Bnouham M, Ziyyat A (2000) Acute diuretic, natriuretic and hypotensive effects of a continuous perfusion of aqueous extract of Urtica dioïca in the rat. J Ethnopharmacol 73:95–100. https://doi.org/10.1016/s0378-8741(00)00270-1
Upton R (2013) Stinging nettles leaf (Urtica dioïca L.): extraordinary vegetable medicine. J Herb Med 3:9–38. https://doi.org/10.1016/j.hermed.2012.11.001
Vajic UJ, Grujic-Milanovic J, Miloradovic Z, Jovovic D, Ivanov M, Karanovic D, Savikin K, Bugarski B, Mihailovic-Stanojevic N (2018) Urtica dioïca L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. Phytomedicine 46:39–45. https://doi.org/10.1016/j.phymed.2018.04.037
Zebib B, Merah O (2017) Satureja myrtifolia (Boiss. & Hohen.) Lebanese wild plant, as a resource of natural antioxidants. Trends Phytochem Res 1(2):103–108
Zeković Z, Cvetanović A, Švarc-Gajić J, Gorjanović S, Sužnjević D, Mašković P, Đurović S (2017) Chemical and biological screening of stinging nettle leaves extracts obtained by modern extraction techniques. Ind Crop Prod 108:423–430. https://doi.org/10.1016/j.indcrop.2017.06.055
Zenão S, Aires A, Dias C, Saavedra MJ, Fernandes C (2017) Antibacterial potential of Urtica dioïca and Lavandula angustifolia extracts against methicillin resistant Staphylococcus aureus isolated from diabetic foot ulcers. J Herb Med 10:53–58. https://doi.org/10.1016/j.hermed.2017.05.003
Author information
Authors and Affiliations
Contributions
OM, Z-ED and BZ contributed to conceptualization, design, writing—original draft preparation,review and editing, BZ and OM were involved in methodology, OM and Z-ED contributed to formal analysis and investigation and OM was involved in statistical analyses.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no conflict of interest.
Rights and permissions
About this article
Cite this article
Merah, O., Djazouli, ZE. & Zebib, B. Aqueous Extract of Algerian Nettle (Urtica dioïca L.) as Possible Alternative Pathway to Control Some Plant Diseases. Iran J Sci Technol Trans Sci 45, 463–468 (2021). https://doi.org/10.1007/s40995-021-01061-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40995-021-01061-z