Abstract
In this current investigation, zinc oxide nanoparticles (ZnONPs) were synthesized from Croton joufra leaves extract containing zinc nitrate as a precursor. Reducing zinc nitrate to free zinc nanoparticles under the impact of phytoconstituents of Croton joufra aqueous leaves extracts has successfully synthesized Croton joufra zinc oxide nanoparticles (Cj-ZnO NPs). The ultraviolet–visible (UV–Vis) spectroscopy revealed the maximum surface plasmon resonance at 346 nm. The Fourier transform infrared (FT-IR) spectroscopy showed a broad peak at 3269.53 cm−1, 1605.67 cm−1, 1070.14 cm−1, and 1042.35 cm−1 disclosing that the nanoparticle contained O–H carboxylic acid stretching, C = N bond, and C–O stretching vibrations. The highly stable Cj-ZnO NPs are repelled by one another, as exhibited by the negative charge of the zeta potential value (− 22.9 mV). The morphological characteristics of the synthesized nanoparticle were analyzed with X-ray diffraction (XRD), field emission scanning electron microscopy–energy dispersive X-ray (FESEM-EDX), scanning electron microscopy (SEM), transmittance electron microscopy (TEM), and differential scanning calorimetry (DSC) techniques. The synthesized Cj-ZnO NPs showed in vitro antidiabetic activity with an IC50 value of 774.86 µg/ml, while metformin was used as a standard drug with an IC50 value of 538.24 µg/ml. This study demonstrates the possibility of using Cj-ZnONPs to treat diabetes.
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References:
Roco, M.C.: Principles of convergence in nature and society and their application: from nanoscale, digits, and logic steps to global progress. J. Nanopart. Res. 22(11), 1–27 (2020)
Lata, S., Sharma, G., Joshi, M., Kanwar, P., Mishra, T.: Role of nanotechnology in drug delivery. Int J Nanotechnol Nanosci. 5, 1–29 (2017)
Becheri, A., Dürr, M., Lo Nostro, P., Baglioni, P.: Synthesis and characterization of zinc oxide nanoparticles: application to textiles as UV-absorbers. J. Nanopart. Res. 10(4), 679–689 (2008)
Balasubramaniam, B., PrateekRanjan, S., Saraf, M., Kar, P., Singh, S.P., Thakur, V.K., Singh, A., Gupta, R.K.: Antibacterial and antiviral functional materials: chemistry and biological activity toward tackling COVID-19-like pandemics. ACS Pharmacol. Translational Sci. 4(1), 8–54 (2020)
Davatgaran-Taghipour, Y., Masoomzadeh, S., Farzaei, M.H., Bahramsoltani, R., Karimi-Soureh, Z., Rahimi, R., Abdollahi, M.: Polyphenol nanoformulations for cancer therapy: experimental evidence and clinical perspective. Int. J. Nanomed. 12, 2689 (2017)
Nuruzzaman, M.D., Rahman, M.M., Liu, Y., Naidu, R.: Nanoencapsulation, nano-guard for pesticides: a new window for safe application. J. Agric. Food Chem. 64(7), 1447–1483 (2016)
Brigger, I., Dubernet, C., Couvreur, P.: Nanoparticles in cancer therapy and diagnosis. Adv. Drug Deliv. Rev. 64, 24–36 (2012)
Kadian, R.: Nanoparticles: a promising drug delivery approach. Asian J Pharm Clin Res. 11(1), 30–35 (2018)
Subramaniam, V.D., Murugesan, R., Pathak, S.: Assessment of the cytotoxicity of cerium, tin, aluminum, and zinc oxide nanoparticles on human cells. J. Nanopart. Res. 22(12), 1–5 (2020)
Manna, K., Somraj Singh, W., Das, L., Reang, M., Das, M., Maiti, D.: Microwave assisted biogenic synthesis of metal nanoparticles using plant extract: characterization and antimicrobial activity. Current Bionanotechnol. 1(2), 87–94 (2015)
Bhande, R.M., Khobragade, C.N., Mane, R.S., Bhande, S.: Enhanced synergism of antibiotics with zinc oxide nanoparticles against extended spectrum β-lactamase producers implicated in urinary tract infections. J. Nanopart. Res. 15(1), 1–3 (2013)
Mao, X., Xu, J., Cui, H.: Functional nanoparticles for magnetic resonance imaging. Wiley Interdisciplinary Rev.: Nanomed. Nanobiotechnol. 8(6), 814–841 (2016)
Wen, M.M., El-Salamouni, N.S., El-Refaie, W.M., Hazzah, H.A., Ali, M.M., Tosi, G., Farid, R.M., Blanco-Prieto, M.J., Billa, N., Hanafy, A.S.: Nanotechnology-based drug delivery systems for Alzheimer’s disease management: technical, industrial, and clinical challenges. J. Control. Release 245, 95–107 (2017)
Sportelli, M.C., Scarabino, S., Picca, R.A., Cioffi, N.: Recent Trends in the Electrochemical Synthesis of Zinc Oxide Nano-Colloids. In: CRC Concise Encyclopedia of Nanotechnology; CRC Press, pp. 1158–1172. LLC, Boca Raton, FL, USA (2015)
Agarwal, H., Venkat Kumar, S., Rajeshkumar, S.: A review on green synthesis of zinc oxide nanoparticles—an eco-friendly approach. Resour. Effic. Technol. 3, 406–413 (2017)
Srivastava, S., Usmani, Z., Atanasov, A.G., Singh, V.K., Singh, N.P., Abdel-Azeem, A.M., Prasad, R., Gupta, G., Sharma, M., Bhargava, A.: Biological nanofactories: using living forms for metal nanoparticle synthesis. Mini Rev. Medic. Chem. 21, 245–265 (2021)
Ahmad, F., Al-Douri, Y., Kumar, D., Ahmad, S.: Metal-oxide powder technology in biomedicine. In: Metal Oxide Powder Technologies, pp. 121–168. Elsevier, Amsterdam (2020)
Happy, A., Soumya, M., Kumar, S.V., Rajeshkumar, S., Sheba, R.D., Lakshmi, T., Nallaswamy, V.D.: Phyto-assisted synthesis of zinc oxide nanoparticles using Cassia alata and its antibacterial activity against Escherichia coli. Biochem. Biophys. Rep. 17, 208–211 (2019)
Schuster, D.P., Duvuuri, V.: Diabetes mellitus. Clin. Podiatr. Med. Surg. 19(1), 79–107 (2002)
San, T.K.: The current and future perspectives of zinc oxide nanoparticles in the treatment of diabetes mellitus. Life Sci. 239, 117011 (2019)
Paul, S., Majumdar, M.: In-Vitro antidiabetic propensities, phytochemical analysis, and mechanism of action of commercial antidiabetic polyherbal formulation “mehon.” InProceedings 79(1), 1–7 (2020)
Mishra, P.K., Mishra, H., Ekielski, A., Talegaonkar, S., Vaidya, B.: Zinc oxide nanoparticles: a promising nanomaterial for biomedical applications. Drug Discovery Today 22(12), 1825–1834 (2017)
Singh, K.R., Nayak, V., Singh, J., Singh, A.K., Singh, R.P.: Potentialities of bioinspired metal and metal oxide nanoparticles in biomedical sciences. RSC Adv. 11(40), 24722–24746 (2021)
Surwade, P., Luxton, T., Clar, J., Xin, F., Shah, V.: Impact of the changes in bacterial outer membrane structure on the anti-bacterial activity of zinc oxide nanoparticles. J. Nanopart. Res. 22(2), 1–8 (2020)
Chen, S., Zhang, Q., Hou, Y., Zhang, J., Liang, X.J.: Nanomaterials in medicine and pharmaceuticals: nanoscale materials developed with less toxicity and more efficacy. European J. Nanomed. 5(2), 61–79 (2013)
Azwanida, N.N.: A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants. 4(196), 2167–2412 (2015)
Elumalai, K., Velmurugan, S., Ravi, S., Kathiravan, V., Ashokkumar, S.: Green synthesis of zinc oxide nanoparticles using Moringa oleifera leaf extract and evaluation of its antimicrobial activity. Spectrochimica Acta Part A: Mol. Biomol. Spectro. 143, 158–164 (2015)
Khouri, S., Shams, M., Tam, K.C.: Determination and prediction of physical properties of cellulose nanocrystals from dynamic light scattering measurements. J. Nanopart. Res. 16(7), 1–4 (2014)
Wickramaratne, M.N., Punchihewa, J.C., Wickramaratne, D.B.: In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina. BMC Complement. Altern. Med. 16(1), 1–5 (2016)
AAT Bioquest, Inc. (2022, July 5). Quest Graph™ IC50 Calculator. AAT Bioquest. https://www.aatbio.com/tools/ic50-calculator.
Khademalrasool, M., Farbod, M., Talebzadeh, M.D.: Investigation of shape effect of silver nanostructures and governing physical mechanisms on photo-activity: zinc oxide/silver plasmonic photocatalyst. Adv. Powder Technol. 32(6), 1844–1857 (2021)
Boluk, Y., Danumah, C.: Analysis ofcellulose nanocrystal rod lengths by dynamic lightscattering and electron microscopy. J. Nanopart. Res. (2014). https://doi.org/10.1007/s11051-013-2174-4
Lim, Y.H., Chew, I.M., Choong, T.S., Tan, M.C., Tan, K.W.: NanoCrystalline Cellulose isolated from oil palm empty fruit bunch and its potential in cadmium metal removal. InMATEC Web Confe (2016). https://doi.org/10.1051/matecconf/20165904002)
Acknowledgements
We are grateful to the Tripura University (A Central University), Suryamaninagar, Tripura, India for providing us with the laboratory facilities in association with the chemicals along with the FT-IR, UV–Vis spectroscopy, and FESEM–EDAX analysis for successfully completing the research work. We would also like to acknowledge Institute of Advanced Study in Science and Technology (IASST), Guwahati, Assam, India for carrying out XRD, TEM, and DLS analysis.
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Ikbal, A.M.A., Rajkhowa, A., Singh, W.S. et al. Green synthesis of zinc oxide nanoparticles using Croton joufra leaf extract, characterization and antidiabetic activity. Int Nano Lett 13, 251–260 (2023). https://doi.org/10.1007/s40089-023-00401-8
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DOI: https://doi.org/10.1007/s40089-023-00401-8