Abstract
Functional properties of the Lactobacillus fermentum CFR 2195, isolated from healthy infant feces have been evaluated. The adherence of L. fermentum to HT-29 and Caco-2 cell-lines were found to be 197.66 ± 15.62 and 100.33 ± 15.69 per 100 cells, respectively. The effect of different concentrations of FOS (0.5, 1.0, 1.5, 2.0 and 2.5%) on the growth rate of L. fermentum was checked and 2.0% FOS was selected for further studies. The synbiotic preparation containing L. fermentum and FOS exhibited significant antimicrobial activity against a few tested common food borne pathogens. The proteolytic activity of the L. fermentum was significant and the total amino acid content in milk fermented with L. fermentum was 555 mg/l. In addition, it was found to produce 29.45 ng vitamin B12/g dry biomass in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h.
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References
Bernet MF, Brassart D, Neeser JR, Servin AL (1993) Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogens-cell interactions. Appl Environ Microbiol 59:4121–4128
Chopin A (1993) Organization and regulation of genes for amino acid biosynthesis in lactic acid bacteria. FEMS Microbiol Rev 12:21–37
Coconnier MH, Klaenhammer T, Kerneis S, Bernet MF, Servin A (1992) Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucus-secreting cell lines in culture. Appl Environ Microbiol 58:2034–2039
Collins MD, Gibson GR (1999) Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut. Am J Clin Nutr 69:1052S–1057S
Fooks LJ, Gibson GR (2002) In vitro investigations of the effect of probiotics and prebiotics on selected human intestinal pathogens. FEMS Microbiol Ecol 39:67–75
Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota—introducing the concept of prebiotics. J Nutr 125:1401–1412
Girishkumar B, Prapulla SG (2010) Beneficial properties of lactic acid bacteria isolated from the breast fed infants fecal flora: in vitro evidences. Asian J Microbiol Biotechnol Environ Sci 12:887–897
Handan K, Robert WH (2000) Fermentation of Fructooligosaccharides by lactic acid bacteria and bifidobacteria. Appl Environ Microbiol 2682–2684
Heudi O, Kilinc T, Fontannaz P, Marleyc E (2006) Determination of vitamin B12 in food products and in premixes by reverse phase high performance liquid chromatography and immunoaffinity extraction. J Chromatogr A 1101:63–68
Joshi VK, Sharma S (2010) Preparation and evaluation of sauces from lactic acid fermented vegetables. J Food Sci Technol 47:214–218
Lee YK, Salminen S (1995) The coming of age of probiotics. Trends Food Sci Technol 6:241–245
Lenaerts K, Bouwman FG, Lamers WH, Renes J, Mariman EC (2007) Comparative proteomic analysis of cell lines and scrapings of the human intestinal epithelium. BMC Genomics 8:1–14
Macfarlane GT, Gibson GR (1997) Carbohydrate fermentation, energy transduction and gas metabolism in the human large intestine. In: Mackie RI, White BA (eds) Gastrointestinal microbiology, vol 1: gastrointestinal ecosystems and fermentations. Chapman and Hall, London, pp 269–318
Madhu AN, Giribhattanavar P, Narayan MS, Prapulla SG (2010) Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC-MS, immunological and microbiological techniques. Biotechnol Lett 32:503–506
Madhukumar MS, Muralikrishna G (2011) Fermentation of xylooligosaccharides obtained from wheat bran and Bengal gram husk by lactic acid bacteria and bifidobacteria. J Food Sci Technol. doi:10.1007/S13197-010-0226-7
Poonawalla FM, Iyengar MRS (1965) Microbiological assay of vitamin B12 in presence of tetracycline. Appl Microbiol 13:755–756
Salminen S, Isolauri E, Salminen E (1996) Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful stains and future challenges. Antonie Leeuwenhoek 70:251–262
Servin AL, Coconnier MH (2003) Adhesion of probiotic strains to the intestinal mucosa and interaction with pathogens. Best Pract Res Clin Gastroenterol 17:741–754
Sridevi J, Halami PM, Vijayendra SVN (2010) Selection of starter cultures for idli batter fermentation and their effect on quality of idlis. J Food Sci Technol 47:557–563
Stabler SP, Allen RH (2004) Vitamin B12 deficiency as a worldwide problem. Annu Rev Nutr 24:299–326
Thomas TD, Mills OE (1981) Proteolytic enzymes of starter bacteria. Neth Milk Dairy J 35:255–273
Tuomola E, Salminen SJ (1998) Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures. Int J Food Microbiol 41:45–51
Wang B, Wei H, Yuan J, Li Q, Li Y, Li N, Li J (2008) Identification of a surface protein from Lactobacillus reuteri JCM1081 that adheres to porcine gastric mucin and human enterocyte-like HT-29 cells. Curr Microbiol 57:33–38
Zhao R, Sun J, Mo H, Zhu Y (2007) Analysis of functional properties of Lactobacillus acidophilus. World J Microbiol Biotechnol 23:195–200
Acknowledgement
The authors are thankful to Director, CFTRI, Mysore, India for supporting the work. Mr. Girishkumar B is grateful to CSIR for the Senior Research Fellowship. Dr. Vijayan, HOD, Department of Biotechnology, JSS College of Pharmacy, Ooty is kindly acknowledged for his help in cell-lines studies.
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Basavanna, G., Prapulla, S.G. Evaluation of functional aspects of Lactobacillus fermentum CFR 2195 isolated from breast fed healthy infants’ fecal matter. J Food Sci Technol 50, 360–366 (2013). https://doi.org/10.1007/s13197-011-0345-9
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DOI: https://doi.org/10.1007/s13197-011-0345-9