Abstract
Sugarcane is the most important sugar crop in China and the world, which originated in tropical and subtropical areas and is a thermophilic crop. Extreme weather occurred frequently in worldwide that caused serious cold or frost damage in recent years, resulting in enormous losses in sugarcane production. Abscisic acid (ABA) regulates much important plant physiological and biochemical processes, and induces tolerance to different stresses including cold or frost damage. This experiment investigated the interrelationship between low temperature induced ABA biosynthesis and endogenous hormone balance using two sugarcane varieties, i.e. the cold tolerant variety GT 28 and cold susceptible variety YL 6. Plants were sprayed with ABA 12 h before cold treatment as opposed to the control group, where no additional substances were added. When the plants in the control group were exposed to cold stress, plant cell membranes were injured, and the GA3 (Gibberellic acid 3) decreased, while the relative electric conductivity, MDA (Malondialdehyde), ABA, the ratio of ABA/GA3, ratio of ABA/IAA (Indole acetic acid), and the ratio of ABA/ZR (Zeatin Riboside) all increased under the cold stress, and there are genotypic differences in response to the contents of proline, ABA and GA, and the ratio of ABA/GA exists between the sugarcane variety GT 28, cold tolerant and variety YL 6, cold susceptible under cold stress. The contents of proline and ABA and the ratio of ABA/GA are higher and the content of GA is lower in the cold tolerant variety, which is the vital physiological basis that caused two sugarcane varieties with different cold resistance. In the ABA treatment, the cell membrane injury was effectively alleviated and the contents of MDA and GA3 decreased, but the contents of proline, ABA, and the ratio of ABA/GA3 increased. The decreasing contents of MDA and GA3, in contrast with the increasing contents of proline, ABA, and ratio of ABA/GA3 in sugarcane leaves from the ABA treatment groups, were important factors that can effectively increase cold stress tolerance in sugarcane plants.
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References
Bates, L.S., R.P. Waldren, and I.D. Teare. 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39(1): 205–207.
Chen, S.Y. 1992. Low temperature stress and peroxidation of membrane lipid in Sugarcane. Journal of Fujian Agriculture and Forestry University (Natural Science Edition) 21(2): 22–26.
Deng, X.K., D.R. Qiao, L. Li, X. Yu, N.S. Zhang, G.P. Lei, and Y. Cao. 2005. The effect of chilling stress on physiological characters of Medicago sativa. Journal of Sichuan University (Natural Science Edition) 42(1): 190–194.
Donald, E.R., and E.K. Kathryn. 2001. How gibberellin regulates plant growth and development: a molecular genetic analysis of gibberellin signaling. Annual Review of Plant Physiology and Plant Molecular Biology 52: 67–88.
Fridovich, L. 1978. The biology of oxygen radicals. Science 201: 875–880.
Guy, C.L. 1990. Cold acclimation and freezing stress tolerance: role of protein metabolism. Annual Review of Plant Physiology and Plant Molecular Biology 41: 187–223.
He, H.Y., L. Xue, L.P. Tian, and Y.L. Chen. 2008. Effect of low temperature stress on the chlorophyll contents and chlorophyll fluorescence parameters in muskmelon seedling leaves. Northern Horticulture 4: 121–127.
Huang, Y.Z., J.Y. Xu, C.J. Chen, and X.Y. He. 2002. Comparative tests on drought resistance and frost resistance of several new sugarcane varieties. Journal of Guangxi Agriculture and Biological Science 21(2): 101–104.
Lang, V., E. Mantyla, B. Welin, B. Sundberg, and E.T. Palva. 1994. Alterations in water status, endogenous abscisic acid content, and expression of rabl8 gene during the development of freezing tolerance in Arabidopsis thaliana. Plant Physiology 104: 1341–1349.
Li, Y.R., and L.T. Yang. 2009. New developments in sugarcane industry and technologies in China since 1990s. Southwest China Journal of Agricultural Sciences 22(5): 1469–1476.
Ohkawa, H., N. Ohishi, and Y. Yagi. 1979. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Analytical Biochemistry 95(2): 351–358.
Perras, M., and F. Sarhan. 1989. Synthesis of freezing tolerance proteins in leaves, crown and roots during cold acclimation of wheat. Plant Physiology 89: 577–585.
Qiu, Y.P., and D.Q. Yu. 2009. Over-expression of the stress-induced OsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis. Environmental and Experimental Botany 65(1): 35–47.
Shi, C.J., Y. Liu, and T. Jing. 2006. Review on Stress-resistance of phytohormone. World Forestry Research 5(19): 21–26.
Sun, F., L.T. Yang, X.N. Xie, G.L. Liu, and Y.R. Li. 2012. Effect of chilling stress on physiological metabolism in chloroplasts of seedlings of sugarcane varieties with different chilling resistance. Acta Agronomica Sinica 38(4): 1–8.
Wang, A.G., C.B. Shao, G.H. Luo, J.Y. Guo, and H.G. Liang. 1988. Senescence and peroxidation of membrane lipid in mitochondria of soybean hypocotyl. Acta Phytophysiologica Sinica 14(3): 269–273.
Wang, X., J. Yu, Y. Yang, J. Chang, and Z.F. Li. 2009. Changes of endogenous hormones of winter wheat varieties with different cold-resistances under low temperature. Journal of Triticeae Crops 29(5): 827–831.
Xin, Z., and P.H. Li. 1992. Abscisic acid-induced chilling tolerance in maize suspension-cultured cells. Plant Physiology 99: 707–711.
Zhang, B.Q., L.T. Yang, and Y.R. Li. 2011. Comparison of physiological and biochemical characteristics related to cold resistance in sugarcane under field conditions. Acta Agronomica Sinica 37(3): 496–505.
Zhang, M.S., B. Xie, and F. Tan. 2002. Relationship between changes on endogenous hormone of sweetpotato under water stress and drought resistance. Scientia Agricultura Sinica 35(5): 498–501.
Zhang, Y.S., X. Huang, and Y.F. Chen. 2009. Plant physiology experiment, 128–129. Beijing: High Education Press.
Zhou, B.Y., and Z.F. Guo. 2005. Effect of ABA and its biosynthesis inhibitor on chilling resistance and anti-oxidant enzymes activity. Acta Pratacultural Science 14(6): 94–96.
Zhu, Q.Z. 1995. Study on the identification of cold resistance in sugarcane. Guangxi Agricultural Sciences 6: 264–265.
Acknowledgments
The present study was supported by the Grants from the National High Technology Research and Development Program (“863” Program) of China (2013AA102604), International Scientific Cooperation Program of China (2013DFA31600), the National Key Technology R&D Program (2008BADB8B00), Guangxi Special Funds for Bagui Scholars and Distinguished Experts (2013), Guangxi Natural Science Foundation (2011GXNSFF018002, 2012GXNSFDA053011, 2013NXNSFAA019073, 2014GXNSFBA118087), and Guangxi R&D Program (Gui Ke Chan 1123008-1, Gui Ke Gong 1222009-1B, Gui Ke He 1347004-2).
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Huang, X., Chen, MH., Yang, LT. et al. Effects of Exogenous Abscisic Acid on Cell Membrane and Endogenous Hormone Contents in Leaves of Sugarcane Seedlings under Cold Stress. Sugar Tech 17, 59–64 (2015). https://doi.org/10.1007/s12355-014-0343-0
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DOI: https://doi.org/10.1007/s12355-014-0343-0