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Magnetic Chitosan Nanocomposite Used as Cleanup Material to Detect Chloramphenicol in Milk by GC-MS

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Abstract

A novel method for determination of chloramphenicol in milk samples was introduced. Magnetic Fe3O4@chitosan nanocomposites were synthesized by adding chitosan to the surface of magnetic Fe3O4 using glutaraldehyde as cross-linker. Extraction with ethyl acetate, the study used the magnetic Fe3O4@CS as cleanup materials, without an additional cleanup step, to detect chloramphenicol in milk samples. To obtain maximal cleanup efficiency, several parameters were investigated including amount of magnetic materials, pH of the solution, purification time, and temperature. Under the optimal conditions (the amount of 100 mg/g of magnetic materials, pH = 5, 10 min, and 20 °C), the sensitivity of the proposed method had improved about tenfold than that of without magnetic materials. Moreover, the decision limits and detection capability were 0.05 and 0.11 μg/kg, respectively, which were comparable to those measured by most methods. The rapid, simple, solvent-saving, and efficient method was proved to be robust in monitoring [d(−)-theo-2-dichloroacetamido-1-p-nitrophenyl-1,3-propanediol] in milk samples.

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References

  • Alizadeh T, Ganjali MR, Zare M, Norouzi P (2012) Selective determination of chloramphenicol at trace level in milk samples by the electrode modified with molecularly imprinted polymer. Food Chem 130:1108–1114

    Article  CAS  Google Scholar 

  • Biancardi A (1997) Determination of aflatoxin M1 residues in milk: a comparative assessment of ELISA and IAC-HPLC methods. Ind Aliment 36:870–876

    CAS  Google Scholar 

  • Chen X, Lee CM, Park H (2003) O/W emulsification for the self-aggregation and nanoparticle formation of linoleic acids modified chitosan in the aqueous system. J Agric Food Chem 51:3135–3139

    Article  CAS  Google Scholar 

  • Chen H, Ying J, Chen H, Huang J, Liao L (2008) LC determination of chloramphenicol in honey using dispersive liquid–liquid microextraction. Chromatographia 68:629–634

    Article  CAS  Google Scholar 

  • D’Aoust JY (1994) Salmonella and the international food trade. Int J Food Microbiol 24:11–31

    Article  Google Scholar 

  • Ding SY, Shen JZ, Zhang SX, Jiang HY, Sun ZW (2005) Determination of chloramphenicol residue in fish and shrimp tissues by gas chromatography with microcell electron capture detector. J AOAC Int 88:57–60

    CAS  Google Scholar 

  • Dong H, Ren X (2007) Adsorption effects of magnetic microsphere on protein in soy whey wastewater. J Food Sci 28(7):205–207

    CAS  Google Scholar 

  • Fujita K, Ito H, Nakamura M, Watai M, Taniguchi M (2008) Determination of chloramphenicol residues in bee pollen by liquid chromatography/tandem mass spectrometry. J AOAC Int 91(5):1103–1109

    CAS  Google Scholar 

  • Gasilova NV, Eremin SA (2010) Determination of chloramphenicol in milk by a fluorescence polarization immunoassay. J Anal Chem 65(3):255–259

    Article  CAS  Google Scholar 

  • Gaudin V, Cadieu N, Maris P (2003) Inter-laboratory studies for the evaluation of ELISA kits for the detection of chloramphenicol residues in milk and muscle. Food Agric Immunol 15:143–157

    Article  CAS  Google Scholar 

  • Gikas E, Kormali P, Tsipi D, Tsarbopoulos A (2004) Development of a rapid and sensitive SPE-LC-ESI MS/MS method for the determination of chloramphenicol in seafood. J Agric Food Chem 52:1025–1030

    Article  CAS  Google Scholar 

  • Hamscher G, Priess B, Nau H, Panariti E (2005) Determination of colchicine residues in sheep serum and milk using high-performance liquid chromatography combined with electrospray ionization ion trap tandem mass spectrometry. Anal Chem 77:2421–2425

    Article  CAS  Google Scholar 

  • Huang J, Zhang H, Feng Y (2006) Chloramphenicol extraction from honey, milk, and eggs using polymer monolith microextraction followed by liquid chromatography-mass spectrometry determination. J Agric Food Chem 54:9279–9286

    Article  CAS  Google Scholar 

  • Hwang D, Damodaran S (1995) Selective precipitation and removal of lipids from cheese whey using chitosan. J Agric Food Chem 43:33–37

    Article  CAS  Google Scholar 

  • Li H, Ruan J, Huang B, Wang Y (2004) Preparation and characterization of magnetic chitosan nanoparticles. J Cent South Univ 35(2):175–179

    CAS  Google Scholar 

  • Liu X, Hu Q, Fang Z, Zhang X, Zhang B (2009) Magnetic chitosan nanocomposites: a useful recyclable tool for heavy metal ion removal. Langmuir 25:3–8

    Article  CAS  Google Scholar 

  • Long AR, Hsieh LC, Bello AC, Malbrough MS, Short CR, Barker SA (1990) Method for the isolation and liquid chromatographic determination of chloramphenicol in milk. J Agric Food Chem 38:427–429

    Article  CAS  Google Scholar 

  • Mamani MCV, Reyes FGR, Rath S (2009) Multiresidue determination of tetracyclines, sulphonamides and chloramphenicol in bovine milk using HPLC-DAD. Food Chem 117:545–552

    Article  CAS  Google Scholar 

  • Meng J, Shi C, Wei B, Yu W, Deng C, Zhang X (2011) Preparation of Fe3O4@C@PANI magnetic microspheres for the extraction and analysis of phenolic compounds in water samples by gas chromatography–mass spectrometry. J Chromatogra A 1218:2841–2847

    Article  CAS  Google Scholar 

  • Moragues F, Igualada C, León N (2012) Validation of the determination of chloramphenicol residues in animal feed by liquid chromatography with an ion trap detector based on European Decision 2002/657/EC. Food Anal Methods 5:416–421

    Article  Google Scholar 

  • Peng ZG, Hidajat K, Uddin MS (2004) Adsorption of bovine serum albumin on nanosized magnetic particles. J Colloid Interf Sci 271:277–283

    Article  CAS  Google Scholar 

  • Pengov A, Flsjs VC, Zadnik T, Marinsek J, Pogacnik M (2005) Distribution of chloramphenicol residues in lactating cows following an external application. Anal Chim Acta 529:347–351

    Article  CAS  Google Scholar 

  • Posyniak A, Zmudzki J, Niedzielska J (2003) Evaluation of sample preparation for control chloramphenicol residues in porcine tissues by enzyme-linked immunosorbent assay and liquid chromatography. Anal Chim Acta 483:307–311

    Article  CAS  Google Scholar 

  • Shenridan R, Policastro B, Thomas S, Rice D (2008) Analysis and occurrence of 14 sulfonamide antibacterials and chloramphenicol in honey by solid-phase extraction followed by LC/MS/MS analysis. J Agric Food Chem 56(10):3509–3516

    Article  Google Scholar 

  • Tajik H, Malekinejad H, Razavi-Rouhani SM, Pajouhi MR, Mahmoudi R, Haghnazari A (2010) Chloramphenicol residues in chicken liver, kidney and muscle: A comparison among the antibacterial residues monitoring methods of four plate test, ELISA and HPLC. Food Chem Toxicol 48:2464–2468

    Article  CAS  Google Scholar 

  • Turnipseed SB, Roybal JE, Pfenning AP, Kijak PJ (2003) Use of ion-trap liquid chromatography-mass spectrometry to screen and comfirm drug residues in aquacultured products. Anal Chim Acta 483:373–386

    Article  CAS  Google Scholar 

  • Tyrpenou AE, Rigos GG, Athanassopoulou F (2002) Determination of chloramphenicol residues in gilthead sea bream (Sparus aurata L.) tissues by HPLC-PDA. J Liq Chromatogr Relat Technol 25:655–663

    Article  CAS  Google Scholar 

  • Wang J, Zheng S, Liu F, Liu J, Tang L, Xu Z (2010) Removal of aqueous humic acid by magnetic chitosan microspheres. Chinese J Inorg Chem 26(10):1761–1767

    CAS  Google Scholar 

  • Wang H, Zhou X, Liu Y, Yang H, Guo Q (2011) Simultaneous determination of chloramphenicol and aflatoxin M1 residues in milk by triple quadrupole liquid chromatography-tandem mass spectrometry. J Agric Food Chem 59:3532–3538

    Article  CAS  Google Scholar 

  • Wesongah JO, Murilla A, Guantai N, Elliot C, Fodey T, Cannavan A (2007) A competitive enzyme-linked immunosorbent assay of determination of chloramphenicol. J Vet Pharmacol Ther 30:68–73

    Article  CAS  Google Scholar 

  • Xie MX, Liu Y, Qiu YM, Han J, Liu YZ (2005) Determination of chloramphenicol residue in animal tissues by solid phase extraction and gas chromatography–mass spectrometry. Chinese J Anal Chem 33(1):1–4

    CAS  Google Scholar 

  • Xu J, Yin WW, Zhang YY, Yi J, Meng M, Wang YB et al (2012) Establishment of magnetic beads-based enzyme immunoassay for detection of chloramphenicol in milk. Food Chem 134:2526–2531

    Article  CAS  Google Scholar 

  • Zhang SX, Zhang Z, Shi WM, Eremin SA, Shen JZ (2006) Development of a chemiluminescent ELISA for determining chloramphenicol in chicken muscle. J Agric Food Chem 54(16):5718–5722

    Article  CAS  Google Scholar 

  • Zhou L, Shang C, Liu Z (2011) Acid dye adsorption properties of ethylenediamine-modified magnetic chitosan nanoparticles. Acta Phys-Chim Sin 27(3):677–682

    CAS  Google Scholar 

  • Zhu C, Shen H, Xu R, Wang H, Han J (2007) Adsorption of bovine serum albumin onto magnetic chitosan microspheres. Acta Phys-Chim Sin 23(10):1583–1588

    CAS  Google Scholar 

Download references

Conflict of Interest

Tianshun Liu declares that he has no conflict of interest. Jia Xie declares that she has no conflict of interest. Jianfeng Zhao declares that he has no conflict of interest. Guoxin Song declares that he has no conflict of interest. Yaoming Hu declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects

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Authors and Affiliations

  1. Research Center of Analysis and Measurement, Fudan University, Shanghai, 200433, People’s Republic of China

    Tianshun Liu, Jia Xie, Jianfeng Zhao, Guoxin Song & Yaoming Hu

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  1. Tianshun Liu
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  2. Jia Xie
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  3. Jianfeng Zhao
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  4. Guoxin Song
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  5. Yaoming Hu
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Correspondence to Guoxin Song or Yaoming Hu.

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Liu, T., Xie, J., Zhao, J. et al. Magnetic Chitosan Nanocomposite Used as Cleanup Material to Detect Chloramphenicol in Milk by GC-MS. Food Anal. Methods 7, 814–819 (2014). https://doi.org/10.1007/s12161-013-9686-5

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  • DOI: https://doi.org/10.1007/s12161-013-9686-5

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