[go: up one dir, main page]
Skip to main content
SpringerLink
Log in

Degradation of Di- Through Hepta-Chlorobiphenyls in Clophen Oil Using Microorganisms Isolated from Long Term PCBs Contaminated Soil

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Present work describes microbial degradation of selected polychlorinated biphenyls (PCBs) congeners in Clophen oil which is used as transformer oil and contains high concentration of PCBs. Indigenous PCBs degrading bacteria were isolated from Clophen oil contaminated soil using enrichment culture technique. A 15 days study was carried out to assess the biodegradation potential of two bacterial cultures and their consortium for Clophen oil with a final PCBs concentration of 100 mg kg−1. The degradation capability of the individual bacterium and the consortium towards the varying range of PCBs congeners (di- through hepta-chlorobiphenyls) was determined using GCMS. Also, dehydrogenase enzyme was estimated to assess the microbial activity. Maximum degradation was observed in treatment containing consortium that resulted in up to 97 % degradation of PCB-44 which is a tetra chlorinated biphenyl whereas, hexa chlorinated biphenyl congener (PCB-153) was degraded up to 90 % by the consortium. This indicates that the degradation capability of microbial consortium was significantly higher than that of individual cultures. Furthermore, the results suggest that for degradation of lower as well as higher chlorinated PCB congeners; a microbial consortium is required rather than individual cultures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Bhatt P, Suresh MK, Chakrabarti T (2009) Fate and degradation of POP-hexachlorocyclohexane. Crit Rev Environ Sci Technol 39:655–695

    Article  CAS  Google Scholar 

  2. Sobiecka E, Cedzynska K, Bielski C, Antizar-Ladislao B (2009) Biological treatment of transformer oil using commercial mixtures of microorganisms. Int Biodeterior Biodegradation 63:328–333

    Article  CAS  Google Scholar 

  3. Pieper DH, Seeger M (2008) Bacterial metabolism of polychlorinated biphenyls. J Mol Microbiol Biotechnol 15:121–138

    Article  CAS  PubMed  Google Scholar 

  4. Field JA, Sierra-Alvarez R (2008) Microbial transformation and degradation of polychlorinated biphenyls. Environ Pollut 155:1–12

    Article  CAS  PubMed  Google Scholar 

  5. Dercova K, Seligova J, Dudasova H, Mikulasova M, Silharova K, Tothova L, Hucko P (2009) Characterization of the bottom sediments contaminated with polychlorinated biphenyls: evaluation of ecotoxicity and biodegradability. Int Biodeterior Biodegradation 63:440–449

    Article  CAS  Google Scholar 

  6. Anyasi RO, Atagana HI (2011) Biological remediation of polychlorinated biphenyls (PCBs) in the soil and sediments by microorganisms and plants. Afr J Plant Sci 5:373–389

    CAS  Google Scholar 

  7. Bedard DL (2008) A case study for microbial biodegradation: anaerobic bacterial reductive dechlorination of polychlorinated biphenyls-from sediment to defined medium. Ann Rev Microbiol 62:253–270

    Article  CAS  Google Scholar 

  8. Pieper DH (2005) Aerobic degradation of polychlorinated biphenyls. Appl Microbiol Biotechnol 67:170–191

    Article  CAS  PubMed  Google Scholar 

  9. Gdaniec-Pietryka M, Wolska L, Namies′nik J (2007) Physical speciation of polychlorinated biphenyls in the aquatic environment. Trends Anal Chem 26:1005–1012

    Article  CAS  Google Scholar 

  10. Aslund MLW, Zeeb BA, Rutter A, Reimer KJ (2007) In situ phytoextraction of polychlorinated biphenyl-(PCB) contaminated soil. Sci Total Environ 374:112

    Article  Google Scholar 

  11. Juwarkar AA, Singh SK, Mudhoo A (2010) A comprehensive overview of elements in bioremediation. Rev Environ Sci Biotechnol 9:215–288

    Article  CAS  Google Scholar 

  12. Shah J, Dahanukar N (2012) Bioremediation of organometallic compounds by bacterial degradation. Indian J Microbiol 52:300–304

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Tu C, Tenga Y, Luo Y, Li X, Suna X, Li Z, Liua W, Christie P (2010) Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti. J Hazard Mater 186:1438–1444

    Article  PubMed  Google Scholar 

  14. Borja J, Taleon DM, Auresenia J, Gallardo S (2005) Polychlorinated biphenyls and their biodegradation. Process Biochem 40:1999–2013

    Article  CAS  Google Scholar 

  15. Dubey KV, Charde PN, Meshram SU, Shendre LP, Dubey VS, Juwarkar AA (2012) Surface-active potential of biosurfactants produced in curd whey by Pseudomonas aeruginosa strain-PP2 and Kocuria turfanesis strain-J at extreme environmental conditions. Bioresour Technol 126:368–374

    Article  CAS  PubMed  Google Scholar 

  16. Kaczorek E, Cies′lak K, Bielicka-Daszkiewicz K, Olszanowski A (2012) The influence of rhamnolipids on aliphatic fractions of diesel oil biodegradation by microorganism combinations. Indian J Microbiol 53:84–91

    Article  PubMed Central  PubMed  Google Scholar 

  17. Qureshi A, Kapley A, Purohit HJ (2012) Degradation of 2,4,6-Trinitrophenol (TNP) by Arthrobacter sp. HPC1223 Isolated from effluent treatment plant. Indian J Microbiol 52:642–647

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Rani R, Juwarkar A (2012) Biodegradation of phorate in soil and rhizosphere of Brassica juncea L. (Indian Mustard) by a microbial consortium. Int Biodeterior Biodegradation 71:36–42

    Article  CAS  Google Scholar 

  19. Zermeno-Eguia L, Jan-Roblero J, de la Serna JZ-D, de Leon AV-P, Hernandez-Rodriguez C (2008) Degradation of polychlorinated biphenyl by a consortium obtained from a contaminated soil composed of Brevibacterium, Pandoraea and Ochrobacterim. World J Microbiol Biotechnol 25:165–170

    Google Scholar 

  20. Furukawa K, Hikaru S, Masatoshi G (2004) Biphenyl dioxygenases: functional versatilities and directed evolution. J Bacteriol 186:5189–5196

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Hatamian-Zarmi A, Shojaosadati SA, Vasheghani-Farahani E, Hosseinkhani S, Emamzadeh A (2009) Extensive biodegradation of highly chlorinated biphenyl and Aroclor 1242 by Pseudomonas aeruginosa TMU56 isolated from contaminated soils. Int Biodeterior Biodegradation 63:788–794

    Article  CAS  Google Scholar 

  22. Chikere CB, Okpokwasili GC, Chikere BO (2011) Monitoring of microbial hydrocarbon remediation in the soil. 3 Biotech 1:117–138

    Article  PubMed Central  PubMed  Google Scholar 

  23. Nanekar S, Dhote M, Kashyap S, Singh SK, Juwarkar AA (2013) Microbe assisted phytoremediation of oil sludge and role of amendments: a mesocosm study. Int J Environ Sci Technol. doi:10.1007/s13762-013-0400-3

    Google Scholar 

  24. Gianfreda L, Rao MA, Piotrowska A, Palumbo G, Colombo C (2005) Soil enzyme activities as affected by anthropogenic alterations: intensive agriculture practices and organic pollution. Sci Total Environ 41:265–279

    Article  Google Scholar 

  25. Kaimi E, Mukaidani T, Miyoshi S, Tamaki M (2006) Ryegrass enhancement of biodegradation in diesel-contaminated soil. Environ Exp Bot 55:110–119

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge Dr. S. R. Wate, Director, CSIR-NEERI for his constant support and motivation. We also acknowledge Ms. Sneha Nanekar for her comments and suggestions during the course of work. The first author is grateful to Council of Scientific and Industrial Research (CSIR), India for the award of Senior Research Fellowship (SRF).

Author information

Authors and Affiliations

  1. CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur, 440020, India

    Jitendra K. Sharma, Ravindra K. Gautam, Rashmi R. Misra, Sanjay M. Kashyap, Sanjeev K. Singh & Asha A. Juwarkar

Authors
  1. Jitendra K. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ravindra K. Gautam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rashmi R. Misra
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sanjay M. Kashyap
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sanjeev K. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Asha A. Juwarkar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jitendra K. Sharma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sharma, J.K., Gautam, R.K., Misra, R.R. et al. Degradation of Di- Through Hepta-Chlorobiphenyls in Clophen Oil Using Microorganisms Isolated from Long Term PCBs Contaminated Soil. Indian J Microbiol 54, 337–342 (2014). https://doi.org/10.1007/s12088-014-0459-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-014-0459-7

Keywords

Search

Navigation