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Development of an Engineered Soil Bacterium Enabling to Convert Both Insoluble Inorganic and Organic Phosphate into Plant Available Phosphate and Its Use as a Biofertilizer

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Abstract

Phosphorus (P) is one of the most important nutrient elements for plant growth and metabolism. We previously isolated a P-solubilizing bacterium 9320-SD with the ability to utilize inorganic P and convert it into plant-available P. The present study aims to enhance the P-solubilizing capacity of 9320-SD, as our long-term goal is to develop a more effective P-solubilizing bacterial strain for use as a biofertilizer. In this end, we introduced a bacterial phytase encoding gene into 9320-SD. One randomly selected transformant, SDLiuTP02, was examined for recombinant protein expression and phytase activity, and assessed for its ability to promote plant growth. Our results indicate that SDLiuTP02 is capable of expressing high levels of phytase activity. Importantly, corn seedlings treated with the SDLiuTP02 cell culture exhibited increased rates of photosynthesis, transpiration, and stomatal conductance as well as increased growth rate under laboratory conditions and increased growth rate in pot assays compared to seedlings treated with cell cultures of the parental strain 9320-SD. Field experiments further indicated that application of SDLiuTP02 promoted a greater growth rate in young cucumber plant and a higher foliar chlorophyll level in chop suey greens when compared to 9320-SD treated controls. These results indicate that SDLiuTP02 has the potential to be a more effective P biofertilizer to increase agricultural productivity.

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Acknowledgments

This work was supported in part by the National Natural Science Foundation of China (Grant #31172029), Tianjin Agriculture and Rural Affairs Committee (Grant #0802220),and Tianjin Municipal Science and Technology Program (Grant #12ZCZDNC00600).

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Authors and Affiliations

  1. College of Life Science, Tianjin Normal University, 393 Binshuixidao Road, Xiqing District, Tianjin, 300387, People’s Republic of China

    Lili Liu, Wenya Du, Wenyu Luo, Yi Su & Jiejie Hui

  2. Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin, 300387, People’s Republic of China

    Lili Liu

  3. Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

    Shengwu Ma

Authors
  1. Lili Liu
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  2. Wenya Du
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  3. Wenyu Luo
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  4. Yi Su
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  5. Jiejie Hui
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  6. Shengwu Ma
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Corresponding authors

Correspondence to Lili Liu or Shengwu Ma.

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Wenyu Luo and Wenya Du have contributed equally to this work.

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Liu, L., Du, W., Luo, W. et al. Development of an Engineered Soil Bacterium Enabling to Convert Both Insoluble Inorganic and Organic Phosphate into Plant Available Phosphate and Its Use as a Biofertilizer. Mol Biotechnol 57, 419–429 (2015). https://doi.org/10.1007/s12033-014-9834-1

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  • DOI: https://doi.org/10.1007/s12033-014-9834-1

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