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The oral microbiome profile and biomarker in Chinese type 2 diabetes mellitus patients

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Abstract

Purpose

Oral microbiota maintains a dynamic ecological balance with the host. However, a disruption in this balance can lead to oral diseases such as dental caries and periodontitis. Several studies suggest differences in microbial composition in the oral cavity between patients with T2DM and nondiabetic patients. However, there is inadequate oral microbiome-related data from Chinese patients with T2DM, and the difference in microbiome profile between Chinese patients with T2DM and other ethnicities needs to be investigated further.

Method

Oral swab samples were collected from 280 adult patients with T2DM and 162 healthy controls. Illumina sequencing was performed on oral samples targeting V1–V2 region of 16S rRNA gene and sequence analysis was carried in the QIIME.

Results

Patients with T2DM and healthy cohorts exhibited distinct oral microbial clusters based on principal coordinate analysis (PCoA). The Firmicutes/Bacteroidetes ratio increased in T2DM and T2DM patients presented significantly higher numbers of Neisseria, Streptococcus, Haemophilus, and Pseudomonas genera, and lower numbers of Acinetobacteria compared with healthy controls. When compared with the available published data of oral and gut microbiome associated with T2DM patients, we found the ratio of Firmicutes/Bacteroidetes and the abundance of Haemophilus could be a specific microbial biomarker in Chinese patients with T2DM.

Conclusions

Our study revealed a significant difference in the oral microbiota between T2DM patients and healthy individuals. We identified 25 taxa, including 6 genera, with significant difference in abundance between T2DM and healthy controls.

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Funding

The study was supported by the National Natural Science Foundation of China, under contract No. 31471202 (L.Z.), 81570712 (Z.W.); the Shandong Provincial Key Research and Development Program under contract No. 2016YYSP009 (L.Z.), 2016GSF201009 (Z.W.); Natural Science Outstanding Youth Foundation of Shandong Province, under contract No. JQ201519 (Q.Z.); Major Science and Technology Innovation Project of Shandong Province, under contract No. 2018CXGC1218 and No. 2018CXGC1219; Weihai Technique Extension Project under contract No. 2016GNS023 (L.Z.), L.Z. was also supported by the TaiShan Scholars Program of Shandong Province (No. tshw20120206) and TaiShan Industrial Experts Program (No. tscy20190612).

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Author notes

  1. These authors contributed equally: Bin Chen, Zhe Wang

Authors and Affiliations

  1. Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Medicine and Engineering, School of Chemistry, & Key Laboratory of Big Data-Based Precision Medicine (Beihang University), the Ministry of Industry and Information Technology of the People’s Republic of China, Beihang University, Beijing, China

    Bin Chen & Lei Zhang

  2. Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China

    Zhe Wang & Xinhuan Su

  3. Qingdao Human Microbiome Center, The Affiliated Central Hospital of Qingdao University, Qingdao, China

    Jingwen Wang

  4. College of Life Science, Qilu Normal University, Jinan, China

    Junjie Yang

  5. The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, and The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Children’s Hospital of Shandong University, Jinan, China

    Qunye Zhang

  6. Microbiological Laboratory, Linyi People’s Hospital, Linyi, China

    Lei Zhang

  7. Shandong Institute for Food and Drug Control, Jinan, China

    Lei Zhang

  8. Shandong Children’s Microbiome Center, Qilu Children’s Hospital of Shandong University, Jinan, China

    Lei Zhang

  9. Shandong Institute of Industrial Technology for Health Science and Precision Medicine, Jinan, China

    Lei Zhang

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  1. Bin Chen
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  2. Zhe Wang
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Contributions

L.Z. and Q.Z. designed the study. B.C., Z.W., J.W., X.S., and J.Y. performed measurements and data analysis. Z.W., Q.Z., and X.S. obtained samples and clinical details. B.C. and J.W. wrote the manuscript. All authors have read and critically revised the manuscript.

Corresponding authors

Correspondence to Qunye Zhang or Lei Zhang.

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The authors declare that they have no conflict of interest.

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All procedures performed in the above studies involving human participants were in accordance with the ethical standards of the institution and/or national research committee and with the 1964 Declaration of Helsinki along with its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Chen, B., Wang, Z., Wang, J. et al. The oral microbiome profile and biomarker in Chinese type 2 diabetes mellitus patients. Endocrine 68, 564–572 (2020). https://doi.org/10.1007/s12020-020-02269-6

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