[go: up one dir, main page]
Skip to main content
SpringerLink
Log in

Protective effect of gelatin and gelatin hydrolysate from salmon skin on UV irradiation-induced photoaging of mice skin

  • Published:
Journal of Ocean University of China Aims and scope Submit manuscript

Abstract

The objective of this study was to investigate the effect of gelatin (SG) isolated from salmon skin and its hydrolysate (SGH) on photoaging skin, and the mechanism responsible for anti-photoaging. The average molecular weights of SG and SGH were 65 kDa and 873 Da, respectively. The amino acid compositions of SG and SGH were similar. Both of them were abundant in hydrophobic amino acids. Twenty-five peptides were identified from SGH. SG and SGH could improve UV irradiation-induced pathological changes of macroscopical tissue texture and skin morphology. Hydroxyproline content is an indicator of matrix collagen content, SG and SGH could inhibit the decrease of hydroxyproline content in photoaging skin in a dose dependent manner. In addition, SG and SGH could alleviate UV irradiation-induced oxidative damages to skin by increasing the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), increasing the content of glutathione (GSH) and decreasing the content of malonaldehyde (MDA). Moreover, SG and SGH could enhance immune regulation system by increasing the thymus index. Thus, the anti-photoaging mechanisms of SG and SGH were by inhibiting the depletion of antioxidant defense components, involving in the synthesis of collagen and enhancing the function of immune system. Besides, SGH showed a better result in protecting skin from photoaging than SG.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arnesen, J. A., and Gildberg, A., 2007. Extraction and characterisation of gelatine from Atlantic salmon (Salmo salar) skin. Bioresource Technology, 98: 53–57.

    Article  Google Scholar 

  • Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248–254.

    Article  Google Scholar 

  • Fan, J., Zhuang, Y. L., and Li, B. F., 2013. Effects of collagen and collagen hydrolysate from jellyfish umbrella on histological and immunity changes of mice photoaging. Nutrients, 5: 223–233.

    Article  Google Scholar 

  • Filip, A., Daicoviciu, D., Clichici, S., Bolfa, P., Catoi, C., Baldea, I., Bolojan, L., Olteanu, D., Muresan, A., and Postescu, I., 2011. The effects of grape seeds polyphenols on SKH-1 mice skin irradiated with multiple doses of UV-B. Journal of Photochemistry and Photobiology B: Biology, 105: 133–142.

    Article  Google Scholar 

  • Fujii, T., Okuda, T., Yasui, N., Wakaizumi, M., Ikami, T., and Ikeda, K., 2013. Effects of amla extract and collagen peptide on UVB-induced photoaging in hairless mice. Journal of Functional Foods, 5: 451–459.

    Article  Google Scholar 

  • Grubeck-Loebenstein, B., and Wick, G., 2002. The aging of the immune system. Advances in Immunology, 80: 243–284.

    Article  Google Scholar 

  • Guo, Y. J., Zhang, T., Jiang, B., Miao, M., and Mu, W. M., 2014. The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines. Journal of Functional Foods, 7: 719–726.

    Article  Google Scholar 

  • Gupta, N., Pant, S. C., Vijayaraghavan, R., and Rao, P. L., 2003. Comparative toxicity evaluation of cyanobacterial cyclic peptide toxin microcystin variants (LR, RR,YR) in mice. Toxicology, 188: 285–296.

    Article  Google Scholar 

  • Harnedy, P. A., and FitzGerald, R. J., 2012. Bioactive peptides from marine processing waste and shellfish: A review. Journal of Functional Foods, 4: 6–24.

    Article  Google Scholar 

  • Hou, H., Li, B. F., Zhang, Z. H., Xue, C. H., Yu, G. L., Wang, J. F., Bao, Y. M., Bu, L., Sun, J., Peng, Z., and Su, S. W., 2012. Moisture absorption and retention properties, and activity in alleviating skin photodamage of collagen polypeptide from marine fish skin. Food Chemistry, 135: 1432–1439.

    Article  Google Scholar 

  • Hou, H., Li, B. F., Zhao, X., Zhuang, Y. L., Ren, G. Y., Yan, M. Y., Cai, Y. P., Zhang, X. K., and Chen, L., 2009. The effect of pacific cod (Gadus macrocephalus) skin gelatin polypeptides on UVradiation-induced skin photoaging in ICR mice. Food Chemistry, 115: 945–950.

    Article  Google Scholar 

  • Je, J. Y., Park, P. J., and Kim, S. K., 2005. Antioxidant activity of a peptide isolated from Alaska pollack (Theragra chalcogramma) frame protein hydrolysate. Food Research International, 38: 45–50.

    Article  Google Scholar 

  • Jung, Y. R., Kim, D. H., Kim, S. R., An, H. J., Lee, E. K., Tanaka, T., Kim, N. D., Yokozawa, T., Park, J. K., and Chung, H. Y., 2014. Anti-wrinkle effect of magnesium lithospermate B from Salvia miltiorrhiza BUNGE: Inhibition of MMPs via NF-kB signaling. PloS One, 9: e102689.

    Article  Google Scholar 

  • Kim, S. K., Kim, Y. T., Byun, H. G., Nam, K. S., Joo, D. S., and Shahidi, F., 2001. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin. Journal of Agricultural and Food Chemistry, 49: 1984–1989.

    Article  Google Scholar 

  • Kim, S. Y., Je, J. Y., and Kim, S. K., 2007. Purification and characterization of antioxidant peptide from hoki (Johnius belengerii) frame protein by gastrointestinal digestion. The Journal of Nutritional Biochemistry, 18: 31–38.

    Article  Google Scholar 

  • Kivirikko, K. I., Laitinen, O., and Prockop, D. J., 1967. Modifications of a specific assay for hydroxyproline in urine. Analytical Biochemistry, 19: 249–255.

    Article  Google Scholar 

  • Kondo, S., 2000. The roles of cytokines in photoaging. Journal of Dermatological Science, 23: S30–S36.

    Article  Google Scholar 

  • Korhonen, H., and Pihlanto, A., 2006. Bioactive peptides: Production and functionality. International Dairy Journal, 16: 945–960.

    Article  Google Scholar 

  • Lapsongphon, N., and Yongsawatdigul, J., 2013. Production and purification of antioxidant peptides from a mungbean meal hydrolysate by Virgibacillus sp. SK37 proteinase. Food Chemistry, 141: 992–999.

    Article  Google Scholar 

  • Li, B., Chen, F., Wang, X., Ji, B. P., and Wu, Y. N., 2007. Isolation and identification of antioxidative peptides from porcine collagen hydrolysate by consecutive chromatography and electrospray ionization-mass spectrometry. Food Chemistry, 102: 1135–1143.

    Article  Google Scholar 

  • Li, Y. H., Jiang, B., Zhang, T., Mu, W. M., and Liu, J., 2008. Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate (CPH). Food Chemistry, 106: 444–450.

    Article  Google Scholar 

  • Liang, J., Pei, X. R., Zhang, Z. F., Wang, N., Wang, J. B., and Li, Y., 2010. The protective effects of long-term oral administration of marine collagen hydrolysate from chum salmon on collagen matrix homeostasis in the chronological aged skin of sprague-dawley male rats. Journal of Food Science, 75: H230–H238.

    Article  Google Scholar 

  • Lin, L., and Li, B. F., 2006. Radical scavenging properties of protein hydrolysates from Jumbo flying squid (Dosidicus eschrichitii Steenstrup) skin gelatin. Journal of the Science of Food and Agriculture, 86: 2290–2295.

    Article  Google Scholar 

  • Mendis, E., Rajapakse, N., and Kim, S. K., 2005. Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate. Journal of Agricultural and Food Chemistry, 53: 581–587.

    Article  Google Scholar 

  • Mendis, E., Rajapakse, N., Byun, H. G., and Kim, S. K., 2005. Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects. Life Sciences, 77: 2166–2178.

    Article  Google Scholar 

  • Naylor, E. C., Watson, R. E., and Sherratt, M. J., 2011. Molecular aspects of skin aging. Maturitas, 69: 249–256.

    Article  Google Scholar 

  • Prasad, N. R., Ramachandran, S., Pugalendi, K. V., and Menon, V. P., 2007. Ferulic acid inhibits UV-B-induced oxidative stress in human lymphocytes. Nutrition Research, 27: 559–564.

    Article  Google Scholar 

  • Rajapakse, N., Mendis, E., Byun, H. G., and Kim, S. K., 2005. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. The Journal of Nutritional Biochemistry, 16: 562–569.

    Article  Google Scholar 

  • Ratnam, D. V., Ankola, D., Bhardwaj, V., Sahana, D. K., and Kumar, M., 2006. Role of antioxidants in prophylaxis and therapy: A pharmaceutical perspective. Journal of Controlled Release, 113: 189–207.

    Article  Google Scholar 

  • Ren, S. W., Li, J., Wang, W., and Guan, H. S., 2010. Protective effects of ca3000+CP against ultraviolet-induced damage in HaCaT and MEF cells. Journal of Photochemistry and Photobiology B: Biology, 101: 22–30.

    Article  Google Scholar 

  • Rittié, L., and Fisher, G. J., 2002. UV-light-induced signal cascades and skin aging. Aging Research Reviews, 1: 705–720.

    Article  Google Scholar 

  • Ryu, B., Qian, Z. J., Kim, M. M., Nam, K. W., and Kim, S. K., 2009. Anti-photoaging activity and inhibition of matrix metalloproteinase (MMP) by marine red alga, Corallina pilulifera methanol extract. Radiation Physics and Chemistry, 78: 98–105.

    Article  Google Scholar 

  • Saiga, A. I., Tanabe, S., and Nishimura, T., 2003. Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. Journal of Agricultural and Food Chemistry, 51: 3661–3667.

    Article  Google Scholar 

  • Steenvoorden, D., and Beijersbergen van Henegouwen, G. M., 1997. The use of endogenous antioxidants to improve photoprotection. Journal of Photochemistry and Photobiology B: Biology, 41: 1–10.

    Article  Google Scholar 

  • Sun, L., Zhang, Y. F., and Zhuang, Y. L., 2013. Antiphotoaging effect and purification of an antioxidant peptide from tilapia (Oreochromis niloticus) gelatin peptides. Journal of Functional Foods, 5: 154–162.

    Article  Google Scholar 

  • Tahara, S., Matsuo, M., and Kaneko, T., 2001. Age-related changes in oxidative damage to lipids and DNA in rat skin. Mechanisms of Ageing and Development, 122: 415–426.

    Article  Google Scholar 

  • Tanaka, M., Koyama, Y. I., and Nomura, Y., 2009. Effects of collagen peptide ingestion on UV-B-induced skin damage. Bioscience, Biotechnology, and Biochemistry, 73: 930–932.

    Article  Google Scholar 

  • Wang, J., Wang, Y. S., Dang, X. L., Zheng, X. X., and Zhang, W. Q., 2013. Housefly larvae hydrolysate: Orthogonal optimization of hydrolysis, antioxidant activity, amino acid composition and functional properties. BMC Research Notes, 6: 197.

    Article  Google Scholar 

  • Wang, Y., Wang, J. F., Gao, S., Zhao, Q., Liu, Z. D., Liang, Y. F., and Xue, C. H., 2008. Protective effect of collagen polypeptides from Apostichopus japonicus on the skin of photoaging-model mice induced by ultraviolet irradiation. Journal-China Pharmaceutical University, 39: 64–67.

    Google Scholar 

  • Wu, Y. Y., Tian, Q., Li, L. H., Khan, M. N., Yang, X. Q., Zhang, Z. M., Hu, X., and Chen, S., 2013. Inhibitory effect of antioxidant peptides derived from Pinctada fucata protein on ultraviolet-induced photoaging in mice. Journal of Functional Foods, 5: 527–538.

    Article  Google Scholar 

  • Yang, J. I., Ho, H. Y., Chu, Y. J., and Chow, C. J., 2008. Characteristic and antioxidant activity of retorted gelatin hydrolysates from cobia (Rachycentron canadum) skin. Food Chemistry, 110: 128–136.

    Article  Google Scholar 

  • Zhuang, Y. L., Hou, H., Zhao, X., Zhang, Z. H., and Li, B. F., 2009. Effects of collagen and collagen hydrolysate from jellyfish (Rhopilema esculentum) on mice skin photoaging induced by UVirradiation. Journal of Food Science, 74: H183–H188.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, P. R. China

    Tiejun Chen, Hu Hou, Jiaohan Lu, Kai Zhang & Bafang Li

Authors
  1. Tiejun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hu Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jiaohan Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kai Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bafang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hu Hou.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, T., Hou, H., Lu, J. et al. Protective effect of gelatin and gelatin hydrolysate from salmon skin on UV irradiation-induced photoaging of mice skin. J. Ocean Univ. China 15, 711–718 (2016). https://doi.org/10.1007/s11802-016-2953-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-016-2953-5

Keywords

Search

Navigation