Abstract
Due to excessive proliferation and metastasis, glioma is the most common primary tumor in the central nervous system. Previous reports show simulated microgravity (SMG) has the ability to inhibit the proliferation and migration of cancer. The aim of this study was to evaluate the viability and migration of U251 cells in SMG environment. SMG induced apoptosis of U251 cells. The FAK/RhoA/Rock and FAK/Nek2 signaling events were attenuated by SMG to destabilize actin cytoskeleton and centrosome disjunction, which caused G2/M arrest and inhibition of cell viability and migration. Overexpressed FAK reversed SMG-induced inhibition of viability and migration in U251 cells, which increased downstream RhoA/Rock signaling and Nek2. These findings reveal novel pathways of FAK/RhoA/Rock and FAK/Nek2 are affected by SMG, and highlight an opportunity to expand therapeutic options in a variety of settings.
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We thank Dr. Austin Cape at ASJ Editors for careful reading and feedback.
Funding
The present study was supported by the Youth Science and Technology Innovation Personnel Training Project of Shaanxi Province (No. 2017KJXX-84), the National Natural Science Foundation of China (Nos. 81402055 and 81602494), and the Natural Science Foundation of Shaanxi Province (No. 2016JM8014).
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Editor: Tetsuji Okamoto
Banglian Deng, Rongrong Liu, and Xin Tian are the co-first authors to this article and contribute equally to this research project.
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Deng, B., Liu, R., Tian, X. et al. Simulated microgravity inhibits the viability and migration of glioma via FAK/RhoA/Rock and FAK/Nek2 signaling. In Vitro Cell.Dev.Biol.-Animal 55, 260–271 (2019). https://doi.org/10.1007/s11626-019-00334-7
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DOI: https://doi.org/10.1007/s11626-019-00334-7