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Simulated microgravity alters the metastatic potential of a human lung adenocarcinoma cell line

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Abstract

Simulated microgravity (SM) has been implicated in affecting diverse cellular pathways. Although there is emerging evidence that SM can alter cellular functions, its effect in cancer metastasis has not been addressed. Here, we demonstrate that SM inhibits migration, gelatinolytic activity, and cell proliferation of an A549 human lung adenocarcinoma cell line in vitro. Expression of antigen MKI67 and matrix metalloproteinase-2 (MMP2) was reduced in A549 cells stimulated by clinorotation when compared with the 1×g control condition, while overexpression of each gene improves ability of proliferation and migration, respectively, under SM conditions. These findings suggest that SM reduced the metastatic potential of human lung adenocarcinoma cells by altering the expression of MKI67 and MMP2, thereby inhibiting cell proliferation, migration, and invasion, which may provide some clues to study cancer metastasis in the future.

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Acknowledgments

This work was supported by the Key Pre-Research Foundation of Military Equipment of China (grant no. 9140A26040312JB10078), the Key Program of Medical Research in the Military “12th 5-year Plan”, China (no. BWS12J046), the China Postdoctoral Science Foundation (grant no. 201104776), the Defense Medical Fund of China (grant no. 06Z048), the State Key Laboratory of Space Medicine Fundamentals and Application, the General Financial Grant from the China Postdoctoral Science Foundation (no. 2012M521873), and the China Astronaut Research and Training Center (grant nos. SMFA11A01 and SMFA11K02).

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Authors and Affiliations

  1. Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, Beijing, 100853, China

    De Chang, Yinghua Guo, Xuege Jiang, Yan Liu, Junfeng Wang, Tianzhi Li & Changting Liu

  2. Institute for Medical Device Standardization Administration, National Institute for Food and Drug Control, Beijing, 100050, China

    Huiwen Xu

  3. Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China

    De Chang, Kailong Li & Chunxiao Pan

  4. The State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, 100094, China

    Ming Yuan

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  1. De Chang
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  3. Yinghua Guo
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  5. Yan Liu
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Corresponding author

Correspondence to Changting Liu.

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Editor: T. Okamoto

De Chang and Huiwen Xu contributed equally to this work.

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Chang, D., Xu, H., Guo, Y. et al. Simulated microgravity alters the metastatic potential of a human lung adenocarcinoma cell line. In Vitro Cell.Dev.Biol.-Animal 49, 170–177 (2013). https://doi.org/10.1007/s11626-013-9581-9

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  • DOI: https://doi.org/10.1007/s11626-013-9581-9

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