Abstract
The presented study summarizes laboratory tests results to define the inhibition effect of selected pharmaceuticals on biogas production under anaerobic digestion conditions. Two sets of inhibition tests were realized: (i) with real anaerobic sludge (from municipal wastewater treatment plant (WWTP) where sludge is present and includes a wide spectrum of pharmaceuticals over a long period) and (ii) with laboratory sludge (sludge without pharmaceuticals). Methanogenic tests lasting 20 days were performed with three analgesics (diclofenac, ibuprofen, and tramadol), two antibiotics (amoxicillin and ciprofloxacin), β-blocker (atenolol), three psychoactive compounds (carbamazepine, caffeine, and cotinine), and a mixture of these compounds. All tests were performed with two concentrations of pharmaceuticals (10 μg/L and 500 μg/L). Results of the methanogenic tests showed the different behaviors of the investigated sludges in the presence of individual pharmaceuticals. Stimulation of anaerobic digestion was mostly detected for laboratory (unadapted) sludge (e.g., the addition of ibuprofen at a concentration of 500 μg/L increased biogas production by 61%). On the other hand, pharmaceuticals inhibited biogas production for real sludge (e.g., the addition of ciprofloxacin 500 μg/L decreased biogas production by 52%).
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Appels L, Baeyens J, Degrève J, Dewil R (2008) Principles and potential of the anaerobic digestion of waste-activated sludge. Prog Energy Combust Sci 34:755–781. https://doi.org/10.1016/j.pecs.2008.06.002
Baker DR, Barron L, Kasprzyk-Hordern B (2014) Illicit and pharmaceutical drug consumption estimated via wastewater analysis. Part A: chemical analysis and drug use estimates. Sci Total Environ 487:629–641. https://doi.org/10.1016/j.scitotenv.2013.11.107
Chen Y, Cheng JJ, Creamer KS (2008) Inhibition of anaerobic digestion process: a review. Bioresour Technol 99:4044–4064. https://doi.org/10.1016/j.biortech.2007.01.057
Chen JL, Ortiz R, Steele TWJ, Stuckey DC (2014) Toxicants inhibiting anaerobic digestion: a review. Biotechnol Adv 32:1523–1534. https://doi.org/10.1016/j.biotechadv.2014.10.005
Fedorova G, Randak T, Lindberg RH, Grabic R (2013) Comparison of the quantitative performance of a Q-Exactive high-resolution mass spectrometer with that of a triple quadrupole tandem mass spectrometer for the analysis of illicit drugs in wastewater. Rapid Commun Mass Spectrom 27:1751–1762. https://doi.org/10.1002/rcm.6628
Fountoulakis M, Drilla P, Stamatelatou K, Lyberatos G (2004) Toxic effect of pharmaceuticals on methanogenesis. Water Sci Technol 50:335–340
Gartiser S, Urich E, Alexy R, Kümmerer K (2007) Anaerobic inhibition and biodegradation of antibiotics in ISO test schemes. Chemosphere 66:1839–1848. https://doi.org/10.1016/j.chemosphere.2006.08.040
Golovko O, Koba O, Kodesova R, Fedorova G, Kumar V, Grabic R (2016) Development of fast and robust multiresidual LC-MS/MS method for determination of pharmaceuticals in soils. Environ Sci Pollut Res 23:14068–14077. https://doi.org/10.1007/s11356-016-6487-6
ISO 13641-1 (2003) Water quality – determination of inhibition of gas production of anaerobic bacteria – part 1
Ivanová L, Fáberová M, Mackuľak T, Grabic R, Bodík I (2017) Estimation of amount of selected pharmaceuticals sorbed onto digested sludge from wastewater treatment plant Bratislava-Petržalka. Environ Res 155:31–35. https://doi.org/10.1016/j.envres.2017.02.001
Ivanová L, Mackuľak T, Grabic R, Golovko O, Koba O, Staňová AV, Szabová P, Grenčíková A, Bodík I (2018) Pharmaceuticals and illicit drugs – a new threat to the application of sewage sludge in agriculture. Sci Total Environ 634:606–615. https://doi.org/10.1016/j.scitotenv.2018.04.001
Khan SJ, Ongerth JE (2002) Estimation of pharmaceutical residues in primary and secondary sewage sludge based on quantities of use and fugacity modelling. Water Sci Technol 46:105–113. https://doi.org/10.2166/wst.2002.0065
Kim J, Kim H, Baek G, Lee C (2017) Anaerobic co-digestion of spent coffee grounds with different waste feedstocks for biogas production. Waste Management 60:322–328. https://doi.org/10.1016/j.wasman.2016.10.015
Kwietniewska E, Tys J (2014) Process characteristics, inhibition factors and methane yields of anaerobic digestion process, with particular focus on microalgal biomass fermentation. Renew Sustain Energy Rev 34:491–500. https://doi.org/10.1016/j.rser.2014.03.041
Lallai A, Mura G, Onnis N (2002) The effects of certain antibiotics on biogas production in the anaerobic digestion of pig waste slurry. Bioresour Technol 82:205–208. https://doi.org/10.1016/S0960-8524(01)00162-6
Liu J-L, Wong M-H (2013) Pharmaceuticals and personal care products (PPCPs): a review on environmental contamination in China. Environ Int 59:208–224. https://doi.org/10.1016/j.envint.2013.06.012
Martín J, Santos JL, Aparicio I, Alonso E (2015) Pharmaceutically active compounds in sludge stabilization treatments: anaerobic and aerobic digestion, wastewater stabilization ponds and composting. Sci Total Environ 503–504:97–104. https://doi.org/10.1016/j.scitotenv.2014.05.089
Paíga P, Correia M, Fernandes MJ, Silva A, Carvalho M, Vieira J, Jorge S, Silva JG, Freire C, Delerue-Matos C (2019) Assessment of 83 pharmaceuticals in WWTP influent and effluent samples by UHPLC-MS/MS: hourly variation. Sci Total Environ 648:582–600. https://doi.org/10.1016/j.scitotenv.2018.08.129
Park J, Yamashita N, Park C, Shimono T, Takeuchi DM, Tanaka H (2017) Removal characteristics of pharmaceuticals and personal care products: comparison between membrane bioreactor and various biological treatment processes. Chemosphere 179:347–358. https://doi.org/10.1016/j.chemosphere.2017.03.135
Raposo F, De la Rubia MA, Fernández-Cegrí V, Borja R (2012) Anaerobic digestion of solid organic substrates in batch mode: an overview relating to methane yields and experimental procedures. Renew Sustain Energy Rev 16:861–877. https://doi.org/10.1016/j.rser.2011.09.008
Sanz JL, Rodriguez N, Amils R (1996) The action of antibiotics on the anaerobic digestion process. Appl Microbiol Biotechnol 46:587–592. https://doi.org/10.1007/s002530050865
Stamatelatou K, Frouda C, Fountoulakis M et al (2003) Pharmaceuticals and health care products in wastewater effluents: the example of carbamazepine. Water Sci Technol Water Supply 3:131–137. https://doi.org/10.2166/ws.2003.0054
Stasinakis AS (2012) Review on the fate of emerging contaminants during sludge anaerobic digestion. Bioresour Technol 121:432–440. https://doi.org/10.1016/j.biortech.2012.06.074
Stuckey DC, Owen WF, McCarty PL (1980) Anaerobic toxicity evaluation by batch and semi-continuous assays. J Water Pollut Control Fed 52:720–729
Tran NH, Gin KY-H (2017) Occurrence and removal of pharmaceuticals, hormones, personal care products, and endocrine disrupters in a full-scale water reclamation plant. Sci Total Environ 599–600:1503–1516. https://doi.org/10.1016/j.scitotenv.2017.05.097
van Lier JB, Tilche A, Ahring BK, Macarie H, Moletta R, Dohanyos M, Hulshoff Pol LW, Lens P, Verstraete W (2001) New perspectives in anaerobic digestion. Water Sci Technol 43:1–18
Verlicchi P, Zambello E (2015) Pharmaceuticals and personal care products in untreated and treated sewage sludge: occurrence and environmental risk in the case of application on soil - a critical review. Sci Total Environ 538:750–767. https://doi.org/10.1016/j.scitotenv.2015.08.108
Verlicchi P, Al Aukidy M, Zambello E (2012) Occurrence of pharmaceutical compounds in urban wastewater: removal, mass load and environmental risk after a secondary treatment-a review. Sci Total Environ 429:123–155. https://doi.org/10.1016/j.scitotenv.2012.04.028
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This work was supported by the Slovak Research and Development Agency under the contract no. APVV 0122-12 and APVV 17-119.
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Fáberová, M., Ivanová, L., Szabová, P. et al. The influence of selected pharmaceuticals on biogas production from laboratory and real anaerobic sludge. Environ Sci Pollut Res 26, 31846–31855 (2019). https://doi.org/10.1007/s11356-019-06314-4
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DOI: https://doi.org/10.1007/s11356-019-06314-4