[go: up one dir, main page]
Skip to main content
Springer Nature Link
Log in

Assessment of antibacterial activity of silver nanoparticles on Pseudomonas aeruginosa and its mechanism of action

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Antimicrobial activity of silver nanoparticles is gaining importance due its broad spectrum of targets in cell compared to conventional antimicrobial agents. In this context, a UV photo-reduction method was used for the synthesis and the nanoparticles were characterized by UV–Visible spectroscopy, transmission electron microscopy, atomic force microscopy and thermogravimetric analysis techniques. The antibacterial activity of the synthesized silver nanoparticles was evaluated both in liquid and solid growth media employing various susceptibility assays on Pseudomonas aeruginosa, a ubiquitous bacterium. The dose dependent growth suppression by nanoparticles was studied with well diffusion method. By broth dilution method, the minimum inhibitory concentration (MIC) was found to be 2 μg/ml. It was observed that the bactericidal effect depends both on nanoparticle concentration and number of bacteria present. In our study, we could demonstrate the complete antibiofilm activity of silver nanoparticles at a concentration as low as 1 μg/ml. Our observations substantiated the association of reactive oxygen species and cell membrane damage in the antibacterial mechanism of silver nanoparticles. Our findings suggested that these nanoparticles can be exploited towards the development of potential antibacterial coatings for various biomedical and environmental applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Baker C, Pradhan A, Pakstis L, Pochan DJ, Shah SI (2005) Synthesis and antibacterial properties of silver nanoparticles. J Nanosci Nanotechnol 5:244–249

    Article  CAS  Google Scholar 

  • Bergogne-Berezin E (2004) Pseudomonas and miscellaneous Gram negative bacilli. In: Cohen J, Powderly WG (eds) Infectious diseases, 2nd edn. Edinburgh, Mosby, pp 2203–2226

    Google Scholar 

  • Bjarnsholt T, Kirketerp-Moller K, Kristiansen S, Phipps R, Nielsen AK, Jensen PO, Hoiby N, Givskov M (2007) Silver against Pseudomonas aeruginosa biofilms. APMIS 115:921–928

    Article  CAS  Google Scholar 

  • Cho KH, Park JE, Osaka T, Park SG (2005) The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim Acta 51:956–960

    Article  CAS  Google Scholar 

  • Choi O, Hu Z (2008) Size dependent and reactive oxygen species related nanosilver toxicity to nitrifying bacteria. Environ Sci Technol 42:4583–4588

    Article  CAS  Google Scholar 

  • Djordjevic D, Wiedmann M, McLandsborough LA (2002) Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Appl Environ Microbiol 68:2950–2958

    Article  CAS  Google Scholar 

  • Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 52:662–668

    Article  CAS  Google Scholar 

  • Ghandour W, Hubbard JH, Deistung J, Hughes MN, Poole RK (1998) The uptake of silver ions by Escherichia coli K12: toxic effects and interaction with copper ions. Appl Microbiol Biotechnol 28:559–565

    Google Scholar 

  • Gibbins BL (2009) SilvaGard™: antimicrobial silver nanotechnology treatment for medical devices. http://www.acrymed.com/pdf/SilvaGard%20Technical%20Summary.pdf. Cited 12 October 2009

  • Inoue Y, Hoshino M, Takahashi H, Noguchi T, Murata T, Kanzaki Y, Hamashima H, Sasatsu M (2002) Bactericidal activity of Ag-zeolite mediated by reactive oxygen species under aerated conditions. J Inorg Biochem 92:37–42

    Article  CAS  Google Scholar 

  • Ip M, Lui SL, Poon VKM, Lung I, Burd A (2006) Antimicrobial activities of silver dressings: an in vitro comparison. J Med Microbiol 55:59–63

    Article  CAS  Google Scholar 

  • Kim JS, Kuk E, Yu KN, Kim J, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang C, Kim Y, Lee Y, Jeong DH, Cho M (2007) Antimicrobial effects of silver nanoparticles. Nanomed Nanotechnol Biol Med 3:95–101

    Article  CAS  Google Scholar 

  • Kong H, Jang J (2008) Antibacterial properties of novel poly (methyl methacrylate) nanofiber containing silver nanoparticles. Langmuir 24:2051–2056

    Article  CAS  Google Scholar 

  • Kora AJ, Manjusha R, Arunachalam J (2009) Superior bactericidal activity of SDS capped silver nanoparticles: synthesis and characterization. Mater Sci Eng, C 29:2104–2109

    Article  CAS  Google Scholar 

  • Lei S, Zhang Z, Dang H (2003) A novel method for preparation of silver nanoparticles. Mater Lett 57:3874–3879

    Article  Google Scholar 

  • Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun H, Tam PK, Chiu J, Che C (2007) Silver nanoparticles: partial oxidation and antibacterial activities. J Biol Inorg Chem 12:527–534

    Article  CAS  Google Scholar 

  • Lyczak JB, Schechter PJ (2005) Nanocrystalline silver inhibits antibiotic, antiseptic-resistant bacteria. American Society for Clinical Pharmacology and Therapeutics. Clinical pharmacol Ther P60

  • May TB, Shinabarger D, Maharaj R, Kato J, Chu TL, Devault JD, Roychoudhury S, Zielinski NA, Berry A, Rothmel TRK, Misra TK, Chakrabarty AM (1991) Alginate synthesis by Pseudomonas aeruginosa: a key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients. Clin Microbiol Rev 4:191–206

    CAS  Google Scholar 

  • Melaiye A, Youngs WJ (2005) Silver and its application as an antimicrobial agent. Expert Opin Ther Pat 15:125–130

    Article  CAS  Google Scholar 

  • Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346–2353

    Article  CAS  Google Scholar 

  • Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73:1712–1720

    Article  CAS  Google Scholar 

  • Panacek A, Kvitek L, Prucek R, Kolar M, Vecerova R, Pizurova N, Sharma VK, Nevecna T, Zboril R (2006) Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem B 110:16248–16253

    Article  CAS  Google Scholar 

  • Pedersen K, Hammer AS, Sorensen CM, Heuer OE (2009) Usage of antimicrobials and occurrence of antimicrobial resistance among bacteria from milk. Vet Microbiol 133:115–122

    Article  CAS  Google Scholar 

  • Prijck KD, Nelis H, Coenye T (2007) Efficacy of silver-releasing rubber for the prevention of Pseudomonas aeruginosa biofilm formation in water. Biofouling 23:405–411

    Article  Google Scholar 

  • Raffi M, Hussain F, Bhatti TM, Akhter JI, Hameed A, Hasan MM (2008) Antibacterial characterization of silver nanoparticles against E: coli ATCC-15224. J Mater Sci Technol 24:192–196

    CAS  Google Scholar 

  • Reddy KR, Lee KP, Lee Y, Gopalan AI (2008) Facile synthesis of conducting polymer–metal hybrid nanocomposites by in situ chemical oxidative polymerization with negatively charged metal nanoparticles. Mater Lett 62:1815–1858

    Article  CAS  Google Scholar 

  • Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 275:177–182

    Article  CAS  Google Scholar 

  • Stickler D, Hewett P (1991) Activity of antiseptics against biofilms of mixed bacterial species growing on silicone surfaces. Eur J Clin Microbiol Infect Dis 10:416–421

    Article  CAS  Google Scholar 

  • Tien DC, Tseng KH, Liao CY, Tsung TT (2009) Identification and quantification of ionic silver from colloidal silver prepared by electric spark discharge system and its antimicrobial potency study. J Alloys Compd 473:298–302

    Article  CAS  Google Scholar 

  • Yang FC, Wu KH, Liu MJ, Lin WP, Hu MK (2009) Evaluation of the antibacterial efficacy of bamboo charcoal/silver biological protective material. Mater Chem Phys 113:474–479

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. S. V. Narasimhan, Associate director and Dr. Tulsi Mukherjee, Director, Chemistry group, BARC for their constant support and encouragement for this work.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. National Centre for Compositional Characterisation of Materials (NCCCM), Bhabha Atomic Research Centre, ECIL PO, Hyderabad, 500 062, India

    Aruna Jyothi Kora & J. Arunachalam

Authors
  1. Aruna Jyothi Kora
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Arunachalam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Aruna Jyothi Kora.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kora, A.J., Arunachalam, J. Assessment of antibacterial activity of silver nanoparticles on Pseudomonas aeruginosa and its mechanism of action. World J Microbiol Biotechnol 27, 1209–1216 (2011). https://doi.org/10.1007/s11274-010-0569-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-010-0569-2

Keywords