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Antimicrobial activity of silver nanoparticle colloids of different sizes and shapes against Streptococcus mutans

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Abstract

Silver nanoparticles stabilized with chitosan biopolymer are a new antibacterial agent for treatment of caries. To determine whether the size and morphology of silver nanoparticles in colloidal solution altered their antimicrobial activity, four colored colloids were characterized by ultraviolet–visible (UV–Vis) spectroscopy and transmission electron microscopy (TEM), and their antimicrobial activity [minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)] against reference stocks of Streptococcus mutans ATCC 25175 compared with chlorhexidine and silver diamine fluoride as controls. TEM revealed that the seed solution (SS) contained only spherical nanoparticles with size of 8.7 ± 3.1 nm whereas the other colloids had predominantly spherical nanoparticle shape, although colloid 1 (S1) contained 2.5% triangular nanoparticles. Colloid 2 (S2) had 15.9% triangular and 6.8% cylindrical nanoparticles, while 23.3% of the silver nanoparticles in colloid 3 (S3) were triangular and 1.5% were cylindrical. All the silver nanoparticle colloids showed antimicrobial activity with no statistically significant differences (p > 0.05) in MIC or MBC. The colloids showed antimicrobial activity higher than silver diamine fluoride (p < 0.05) and equivalent to chlorhexidine (p > 0.05). The size and shape of the nanoparticles did not affect the antimicrobial properties of the colored colloids. Thus, the antimicrobial activity of the colloids containing silver nanoparticles against Streptococcus mutans was considered equivalent to that of chlorhexidine, the gold-standard antibiotic in dentistry.

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Abbreviations

UV–Vis:

Ultraviolet–visible

TEM:

Transmission electron microscopy

MIC:

Minimum inhibitory concentration

MBC:

Minimum bactericidal concentration

SS:

Seed solution

S1:

Colloid 1

S2:

Colloid 2

S3:

Colloid 3

NPs:

Nanoparticles

AgNPs:

Silver nanoparticles

SDF:

Silver diamine fluoride

BHI:

Brain heart infusion

SMM:

Spectrophotometric microdilution method

ELISA:

Enzyme-linked immunosorbent assay

ANOVA:

Analysis of variance

SPSS:

Statistical Package for the Social Sciences

SPR:

Surface plasmon resonance

FWHH:

Full-width at half-height

ATCC:

American Type Culture Collection

DNA:

Deoxyribonucleic acid

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Acknowledgements

The authors wish to thank the Brazilian Ministry of Education (CAPES) (no. 001/2010), the Brazilian Ministry of Science and Technology (CNPq) (403179/2012-2), and the State of Pernambuco Department of Science, Technology, and the Environment (FACEPE) (BCT 0143-4.02/11).

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Authors and Affiliations

  1. Center for Strategic Technologies of Northeast, CETENE – INT/MCTI, Rua São Sebastião 417 apto 101 Piedade, Jaboatão dos Guararapes, Recife, PE, Brazil

    Valdeci Elias dos Santos Junior, Miguel Angel Pelagio Flores & André Galembeck

  2. Dental School, Federal University of Paraíba, João Pessoa, Brazil

    Andrea Gadelha Ribeiro Targino & Hilzethe de Luna Freire Pessoa

  3. Laboratorio de Análisis Químicos y Biotecnológicos, Instituto de Investigación, Universidad Técnica de Manabí, Portoviejo, Ecuador

    Joan Manuel Rodríguez-Díaz

  4. Department of Pediatric Dentistry, University of Pernambuco, Recife, Brazil

    Joás Araújo Teixeira, Mônica Vilela Heimer & Aronita Rosenblatt

Authors
  1. Valdeci Elias dos Santos Junior
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  2. Andrea Gadelha Ribeiro Targino
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  3. Miguel Angel Pelagio Flores
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  4. Joan Manuel Rodríguez-Díaz
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  6. Mônica Vilela Heimer
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  7. Hilzethe de Luna Freire Pessoa
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  8. André Galembeck
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  9. Aronita Rosenblatt
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Correspondence to Valdeci Elias dos Santos Junior.

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dos Santos Junior, V.E., Targino, A.G.R., Flores, M.A.P. et al. Antimicrobial activity of silver nanoparticle colloids of different sizes and shapes against Streptococcus mutans . Res Chem Intermed 43, 5889–5899 (2017). https://doi.org/10.1007/s11164-017-2969-5

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  • DOI: https://doi.org/10.1007/s11164-017-2969-5

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