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Improvement of kinetics, yield, and colloidal stability of biogenic gold nanoparticles using living cells of Euglena gracilis microalga

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Abstract

Recent years have witnessed a boom in the biosynthesis of a large variety of nanomaterials using different biological resources among which algae-based entities have been gaining much more attention within the community of material scientists worldwide. In our previously published findings, we explored some factors that governed the biofabrication of gold nanoparticles using living cultures of microalgae, such as the utilized microalgal genera, the phylum they belong to, and the impact of tetrachloroauric acid concentrations on the ability of these strains to perform the biosynthesis of gold nanoparticles once in contact with these cations. As a follow-up, we present in this paper an improvement of the features of bioproduced gold colloids using living cells of Euglena gracilis microalga when this species is grown under either mixotrophic or autotrophic conditions, i.e., exposed to light and grown in an organic carbon-enriched culture medium versus under autotrophic conditions. As an outcome to this alteration, the growth rate of this photosynthetic microorganism is multiplied 7–8 times when grown under mixotrophic conditions compared to autotrophic ones. Therefore, the yield, the kinetics, and the colloidal stability of the biosynthesized gold nanoparticles are dramatically enhanced. Moreover, the shape and the size of the as-produced nano-objects via this biological method are affected. In addition to round-shaped gold nanoparticles, particular shapes, such as triangles and hexagons, appear. These findings add up to the amassed knowledge toward the design of photobioreactors for the scalable and sustainable production of interesting nanomaterials.

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Acknowledgments

SAD thanks the French Ministry of Higher Education and Scientific Research for financial support.

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Authors and Affiliations

  1. Sorbonne Paris Cité, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), UMR 7086, CNRS, Paris-Diderot University, 15 rue Jean de Baïf, 75205, Paris Cedex 13, France

    Si Amar Dahoumane, Fernand Fiévet & Roberta Brayner

  2. School of Life Sciences and Biotechnology, Hacienda San José, Yachay Tech University, San Miguel de Urcuquí, Ecuador

    Si Amar Dahoumane

  3. Département RDDM, UMR 7245, Unité MCAM, Muséum National d’Histoire Naturelle, 57 rue Cuvier, case 39, 57 rue Cuvier, 75005, Paris, France

    Claude Yéprémian, Chakib Djédiat & Alain Couté

  4. UPMC—Paris 06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 place Marcellin Berthelot, 75005, Paris, France

    Thibaud Coradin

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  1. Si Amar Dahoumane
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  2. Claude Yéprémian
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  3. Chakib Djédiat
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  5. Fernand Fiévet
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  7. Roberta Brayner
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Correspondence to Si Amar Dahoumane.

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Dahoumane, S.A., Yéprémian, C., Djédiat, C. et al. Improvement of kinetics, yield, and colloidal stability of biogenic gold nanoparticles using living cells of Euglena gracilis microalga. J Nanopart Res 18, 79 (2016). https://doi.org/10.1007/s11051-016-3378-1

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