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Solvent-partitioned fractions from Ishige okamurae extract inhibit MMP-2 and MMP-9 activities in human fibrosarcoma cells in vitro

  • 9th Asia Pacific Conference on Algal Biotechnology - Bangkok
  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

Matrix metalloproteinases (MMPs) are endopeptidases that take significant roles in extracellular matrix degradation and therefore linked with several complications such as metastasis of cancer progression, oxidative stress, and hepatic fibrosis. Ishige okamurae, a brown alga, has been reported to possess various bioactivities including but not limited to antiviral, antimicrobial, and anti-inflammatory. In this study, the potential of I. okamurae against cancer cell invasion was evaluated through MMP inhibitory effect in HT1080 fibrosarcoma cells in vitro. I. okamurae crude extract was fractionated with organic solvents, namely water (H2O), n-butanol (n-BuOH), 85% aqueous methanol (85% aq. MeOH), and n-hexane (n-Hex). The non-toxicity of fractions was confirmed by MTT assay. All fractions inhibited the enzymatic activities of MMP-2 and MMP-9 according to gelatin zymography assay. Cell migration was also inhibited by 85% aq. MeOH fraction, significantly. In addition, both gene and protein expressions of MMP-2 and MMP-9, and tissue inhibitor of MMPs (TIMPs) were evaluated by RT-PCR and Western blotting, respectively. Fractions suppressed the mRNA and protein levels of MMP-2 and MMP-9 while elevating the TIMP-1 and TIMP-2, H2O fraction being the least effective while 85% aq. MeOH fraction the most. Collectively, 85% aq. MeOH fraction from brown algae could be a potential inhibitor of MMPs, suggestively considering presence of poly-unsaturated fatty acids in high amounts.

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Authors and Affiliations

  1. Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Baegyang-dero 700 beon-gil 140, Sasang-gu, Busan, 46958, Republic of Korea

    Fatih Karadeniz, Seul-Gi Lee, Jung Hwan Oh, Ga Hyun Yu & Chang-Suk Kong

  2. Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Baegyang-dero 700 beon-gil 140, Sasang-gu, Busan, 46958, Republic of Korea

    Fatih Karadeniz & Chang-Suk Kong

  3. Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea

    Mi-Soon Jang

  4. Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University, Busan, 49112, Republic of Korea

    Youngwan Seo

  5. Department of Convergence Study on the Ocean Science and Technology, Ocean Science and Technology School, Korea Maritime and Ocean University, Busan, 49112, Republic of Korea

    Youngwan Seo

Authors
  1. Fatih Karadeniz
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  2. Seul-Gi Lee
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  3. Jung Hwan Oh
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  4. Ga Hyun Yu
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  5. Mi-Soon Jang
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  6. Youngwan Seo
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  7. Chang-Suk Kong
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Corresponding author

Correspondence to Chang-Suk Kong.

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Funding

This research was financially supported by grants from the National Institute of Fisheries Science (R2017061) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2014R1A1A2059310).

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The authors declare that they have no conflict of interest.

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Karadeniz, F., Lee, SG., Oh, J.H. et al. Solvent-partitioned fractions from Ishige okamurae extract inhibit MMP-2 and MMP-9 activities in human fibrosarcoma cells in vitro. J Appl Phycol 30, 121–127 (2018). https://doi.org/10.1007/s10811-017-1228-x

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  • DOI: https://doi.org/10.1007/s10811-017-1228-x

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