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The Effects of Dexamethasone and L-NAME on Acute Lung Injury in Rats with Lung Contusion

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Abstract

The therapeutic efficiency of an anti-inflammatory agent, dexamethasone (DXM), and a nitric oxide synthase (NOS) inhibitor, Nitro-L-arginine methyl ester (L-NAME), in lung tissue injury after lung contusion was investigated. Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), YKL-40, an inflammatory peptide, inducible NOS (iNOS), and Clara cell protein 16 (CC-16) were evaluated. Immunohistochemical analyses were also performed, and the lung tissue was examined histopathologically. The study consisted of eight groups of Sprague-Dawley rats (n = 10 in each group), weighing 250–300 g: (1) control, (2) contusion, (3) control + DXM, (4) contusion + DXM, (5) control + L-NAME (6) contusion + L-NAME, (7) control + DXM + L-NAME, and (8) contusion + DXM + L-NAME. A previously developed lung contusion model was used, in addition to the control group. The rats were administered DXM and L-NAME intraperitoneally (i.p.) at doses of 15 and 60 mg/kg/day, respectively. DXM and L-NAME administration decreased the iNOS level in the contusion groups. DXM increased the levels of YKL-40 and IL-10 in both the control and contusion groups, with higher levels in the contusion groups. L-NAME increased the serum level of IL-10 in the lung contusion groups. DXM increased the synthesis of CC-16 in the control and contusion groups. The combined use of a high-dose steroid and NOS inhibitor resulted in the death of the rats. Steroids can increase the level of cytokines, such as YKL-40 and IL-10, and the synthesis of CC-16 and prevent pneumonia, ALI/ARDS, and sepsis in lung contusion.

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Acknowledgments

This study was supported by the Scientific and Technological Research Council of Turkey (project no. 114S008) project number. We thank the council for its support.

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Authors and Affiliations

  1. Biochemistry Specialist, Biochemistry Laboratory, Gazi State Hospital, Samsun, Turkey

    Ahmet Kozan

  2. Department of Medical Biochemistry, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Nermin Kilic

  3. Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey

    Hasan Alacam

  4. Department of Pediatrics, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Ahmet Guzel & Mehmet Acikgoz

  5. Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, Turkey

    Tolga Guvenc

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  1. Ahmet Kozan
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Correspondence to Ahmet Kozan.

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Kozan, A., Kilic, N., Alacam, H. et al. The Effects of Dexamethasone and L-NAME on Acute Lung Injury in Rats with Lung Contusion. Inflammation 39, 1747–1756 (2016). https://doi.org/10.1007/s10753-016-0409-0

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