Abstract
The basement membrane (BM) proteins laminins, which consist of α, β and γ chains, play critical roles in the maintenance of tissue structures. One of laminin α chains, α3 has two isoforms, the truncated form α3A and the full-sized form α3B. In contrast to α3A laminins, little is known about α3B laminins. To show the histological distribution of the laminin α3B chain, we prepared α3B-specific monoclonal antibodies. Immunohistochemical analysis showed that the α3B chain was colocalized with the α3A, β3 and γ2 chains in the epithelial BMs of the skin, esophagus, breast and lung, suggesting the presence of laminin-3B32 (laminin-5B) and laminin-3A32 (laminin-5A). In the lung alveoli, laminin-3B32 was dominant over laminin-3A32, but vice versa in other epithelial BMs. In contrast, the BMs of blood vessels including capillaries were strongly positive for α3B, but almost or completely negative for α3A, β3 and γ2. α3B was colocalized with β1 and γ1 in these BMs. The α3B chain was scarcely detected in the vessels of malignant skin cancers, though the γ2 and β3 chains were highly expressed in the cancer cells. These results strongly suggest that the laminin α3B chain is widely expressed in vascular BMs of normal tissues, probably as laminin-3B11/3B21 (laminin-6B/7B).
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Abbreviations
- BM:
-
Basement membrane
- ECM:
-
Extracellular matrix
- Lmxyz:
-
Laminin-xyz
- mAb:
-
Monoclonal antibody
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Acknowledgements
We thank Ms. Naoko Kato for technical support. We are grateful to Drs. S. Higashi and K. Yamamoto for helpful discussions. This work was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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Kariya, Y., Mori, T., Yasuda, C. et al. Localization of laminin α3B chain in vascular and epithelial basement membranes of normal human tissues and its down-regulation in skin cancers. J Mol Hist 39, 435–446 (2008). https://doi.org/10.1007/s10735-008-9183-0
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DOI: https://doi.org/10.1007/s10735-008-9183-0